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Literature summary extracted from

  • Liu, D.; Xi, L.; Han, D.; Dou, K.; Su, S.; Liu, J.
    Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6 (2019), Biotechnol. Lett., 41, 583-589 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.1 acetone 5% (v/v), 1.2fold activation Pannonibacter carbonis
3.5.5.1 dithiothreitol 1 mM, 1.2fold activation Pannonibacter carbonis
3.5.5.1 EDTA 1 mM, 1.35fold activation Pannonibacter carbonis
3.5.5.1 glycerin 1% (v/v), 1.3fold activation Pannonibacter carbonis
3.5.5.1 L-cysteine 1 mM, 1.3fold activation Pannonibacter carbonis
3.5.5.1 L-glutathione 1 mM, 1.3fold activation Pannonibacter carbonis
3.5.5.1 SDS 1% (v/v), 1.4fold activation Pannonibacter carbonis
3.5.5.1 Span-80 1% (v/v), 1.2fold activation Pannonibacter carbonis
3.5.5.7 DTT 1 mM, 1.18fold activation Pannonibacter carbonis
3.5.5.7 EDTA 1 mM, 1.35fold activation Pannonibacter carbonis
3.5.5.7 glycerin 1% (v/v), 1.3fold activation Pannonibacter carbonis
3.5.5.7 Isopropanol 1% (v/v), 1.2fold activation Pannonibacter carbonis
3.5.5.7 L-cysteine 1 mM, 1.29fold activation Pannonibacter carbonis
3.5.5.7 L-glutathione 1 mM, 1.27fold activation Pannonibacter carbonis
3.5.5.7 n-hexane 1% (v/v), 1.1fold activation Pannonibacter carbonis
3.5.5.7 SDS 1% (v/v), 1.4fold activation Pannonibacter carbonis
3.5.5.7 Span-80 1% (v/v), 1.2fold activation Pannonibacter carbonis

Application

EC Number Application Comment Organism
3.5.5.1 synthesis the broad range of substrate specificity, the high activity towards aliphatic, aromatic, and arylacetonitriles, and the highly stability, in terms of temperature, pH, and metal ions make the enzyme a promising biocatalyst for mild nitrile hydrolysis Pannonibacter carbonis
3.5.5.7 industry the enzyme is a promising biocatalyst for mild nitrile hydrolysis Pannonibacter carbonis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression in Escherichia coli BL21 (DE3) Pannonibacter carbonis
3.5.5.7 expression in Escherichia coli Pannonibacter carbonis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 BaCl2 5 mM, 35% inhibition Pannonibacter carbonis
3.5.5.1 CaCl2 5 mM, 34% inhibition Pannonibacter carbonis
3.5.5.1 dimethyl sulfoxide 1% (v/v) Pannonibacter carbonis
3.5.5.1 dithiothreitol 5 mM, 37% inhibition Pannonibacter carbonis
3.5.5.1 DMSO 5% (v/v), % inhibition Pannonibacter carbonis
3.5.5.1 ether 5% (v/v), 23% inhibition Pannonibacter carbonis
3.5.5.1 ethyl acetate 5% (v/v), 35% inhibition Pannonibacter carbonis
3.5.5.1 FeCl3 5 mM, 23% inhibition Pannonibacter carbonis
3.5.5.1 FeSO4 5 mM, 47% inhibition Pannonibacter carbonis
3.5.5.1 glycerin 5% (v/v), 52% inhibition Pannonibacter carbonis
3.5.5.1 KCl 5 mM, 58% inhibition Pannonibacter carbonis
3.5.5.1 methanol 5% (v/v), 29% inhibition Pannonibacter carbonis
3.5.5.1 MgCl2 5 mM, 18% inhibition Pannonibacter carbonis
3.5.5.1 n-heptane 5% (v/v), 25% inhibition Pannonibacter carbonis
3.5.5.1 NaCl 5 mM, 26% inhibition Pannonibacter carbonis
3.5.5.1 SDS 5% (v/v), 37% inhibition Pannonibacter carbonis
3.5.5.1 Triton X-100 5% (v/v), 14% inhibition Pannonibacter carbonis
3.5.5.1 ZnCl2 5 mM, 32% inhibition Pannonibacter carbonis
3.5.5.7 BaCl2 5 mM, 35% inhibition Pannonibacter carbonis
3.5.5.7 CaCl2 5 mM, 34% inhibition Pannonibacter carbonis
3.5.5.7 DTT 5 mM, 37% inhibition Pannonibacter carbonis
3.5.5.7 FeCl3 5 mM, 23% inhibition Pannonibacter carbonis
3.5.5.7 FeSO4 5 mM, 47% inhibition Pannonibacter carbonis
3.5.5.7 glycerin 5% (v/v), 52% inhibition Pannonibacter carbonis
3.5.5.7 KCl 5 mM, 58% inhibition Pannonibacter carbonis
3.5.5.7 MgCl2 5 mM, 18% inhibition Pannonibacter carbonis
3.5.5.7 MnCl2 5 mM, 50% inhibition Pannonibacter carbonis
3.5.5.7 n-hexane 5% (v/v), 6% inhibition Pannonibacter carbonis
3.5.5.7 NaCl 5 mM, 26% inhibition Pannonibacter carbonis
3.5.5.7 SDS 5% (v/v), 37% inhibition Pannonibacter carbonis
3.5.5.7 Triton X-100 5% (v/v), 14% inhibition Pannonibacter carbonis
3.5.5.7 ZnCl2 5 mM, 32% inhibition Pannonibacter carbonis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 10.31
-
3-Cyanopyridine pH 7.0, 65°C Pannonibacter carbonis
3.5.5.7 10.31
-
3-Cyanopyridine pH 7.2, 30°C Pannonibacter carbonis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.1 Ag+ 1 mM, 3.2fold activation Pannonibacter carbonis
3.5.5.1 Cr3+ 5 mM, 2.8fold activation Pannonibacter carbonis
3.5.5.1 Cu2+ 5 mM, 1.3fold activation Pannonibacter carbonis
3.5.5.1 Hg2+ 5 mM, 5.2fold activation Pannonibacter carbonis
3.5.5.1 Ni2+ 5 mM, 3fold activation Pannonibacter carbonis
3.5.5.7 AgNO3 1 mM, 1.97fold activation Pannonibacter carbonis
3.5.5.7 CoCl2 1 mM, 1.08fold activation Pannonibacter carbonis
3.5.5.7 CuCl2 1 mM, 1.28fold activation Pannonibacter carbonis
3.5.5.7 HgCl2 1 mM, 3.2fold activation Pannonibacter carbonis
3.5.5.7 NiCl2 1 mM, 1.67fold activation Pannonibacter carbonis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.7 31000
-
SDS-PAGE Pannonibacter carbonis

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Pannonibacter carbonis
-
-
-
3.5.5.1 Pannonibacter carbonis Q4.6
-
-
-
3.5.5.7 Pannonibacter carbonis
-
-
-
3.5.5.7 Pannonibacter carbonis Q4.6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
Pannonibacter carbonis
3.5.5.7
-
Pannonibacter carbonis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2-methylglutaronitrile + H2O
-
Pannonibacter carbonis ? + NH3
-
?
3.5.5.1 3-cyanopyridine + 2 H2O
-
Pannonibacter carbonis nicotinic acid + NH3
-
?
3.5.5.1 4-chlorobutyronitrile + H2O
-
Pannonibacter carbonis 4-chlorobutyrate + NH3
-
?
3.5.5.1 acetonitrile + H2O
-
Pannonibacter carbonis acetic acid + NH3
-
?
3.5.5.1 acrylonitrile + H2O
-
Pannonibacter carbonis acrylic acid + NH3
-
?
3.5.5.1 benzonitrile + 2 H2O
-
Pannonibacter carbonis benzoic acid + NH3
-
?
3.5.5.1 dicyanobutane + H2O
-
Pannonibacter carbonis ? + NH3
-
?
3.5.5.1 dicyanooctane + H2O
-
Pannonibacter carbonis ? + NH3
-
?
3.5.5.1 dodecanenitrile + H2O
-
Pannonibacter carbonis dodecanoic acid + NH3
-
?
3.5.5.1 fumaronitrile + H2O
-
Pannonibacter carbonis fumaric acid + NH3
-
?
3.5.5.1 iminodiacetonitrile + H2O
-
Pannonibacter carbonis iminodiacetic acid + NH3
-
?
3.5.5.1 indole-3-acetonitrile + H2O
-
Pannonibacter carbonis indol-3-acetic acid + NH3
-
?
3.5.5.1 mandelonitrile + H2O
-
Pannonibacter carbonis mandelic acid + NH3
-
?
3.5.5.1 pentanenitrile + H2O
-
Pannonibacter carbonis pentanoic acid + NH3
-
?
3.5.5.1 phenylacetonitrile + 2 H2O
-
Pannonibacter carbonis phenylacetic acid + NH3
-
?
3.5.5.1 phenylenediacetonitrile + H2O
-
Pannonibacter carbonis ? + NH3
-
?
3.5.5.1 phenylpropionitrile + 2 H2O
-
Pannonibacter carbonis phenylpropionic acid + NH3
-
?
3.5.5.1 succinonitrile + H2O
-
Pannonibacter carbonis succinic acid + NH3
-
?
3.5.5.1 thiophene + H2O
-
Pannonibacter carbonis ? + NH3
-
?
3.5.5.7 2-methylglutaronitrile + 4 H2O activity is 2.3fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 2-methylglutaric acid + 2 NH3
-
?
3.5.5.7 2-methylglutaronitrile + 4 H2O activity is 2.3fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 2-methylglutaric acid + 2 NH3
-
?
3.5.5.7 3-cyanopyridine + 2 H2O
-
Pannonibacter carbonis pyridine 3-carboxylic acid + NH3
-
?
3.5.5.7 3-cyanopyridine + 2 H2O
-
Pannonibacter carbonis Q4.6 pyridine 3-carboxylic acid + NH3
-
?
3.5.5.7 3-phenylpropionitrile + 2 H2O activity is 2.15fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 3-phenylpropionic acid + NH3
-
?
3.5.5.7 4-chlorobutyronitrile + 2 H2O activity is 2.44fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis 4-chlorobutanoic acid + NH3
-
?
3.5.5.7 acrylonitrile + 2 H2O activity is 2.2fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis acrylic acid + NH3
-
?
3.5.5.7 acrylonitrile + 2 H2O activity is 2.2fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 acrylic acid + NH3
-
?
3.5.5.7 adiponitrile + 4 H2O activity is 2.13fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis adipic acid + 2 NH3
-
?
3.5.5.7 benzonitrile + 2 H2O activity is 2.24fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis benzoic acid + NH3
-
?
3.5.5.7 benzonitrile + 2 H2O activity is 2.24fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis Q4.6 benzoic acid + NH3
-
?
3.5.5.7 dodecanenitrile + 2 H2O activity is 2.46fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis dodecanoic acid + NH3
-
?
3.5.5.7 fumaronitrile + 4 H2O activity is 2.21fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis fumaric acid + 2 NH3
-
?
3.5.5.7 iminodiacetonitrile + 2 H2O activity is 2.34fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis iminodiacetic acid + NH3
-
?
3.5.5.7 additional information the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles Pannonibacter carbonis ?
-
-
3.5.5.7 additional information the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles Pannonibacter carbonis Q4.6 ?
-
-
3.5.5.7 pentanenitrile + 2 H2O activity is 2.39fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis pentanoic acid + NH3
-
?
3.5.5.7 sebaconitrile + 4 H2O activity is 1.92fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis sebaconic acid + 2 NH3
-
?
3.5.5.7 succinonitrile + 4 H2O activity is 2.64fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis succinic acid + 2 NH3
-
?
3.5.5.7 thiophene-3-carbonitrile + 2 H2O activity is 2.42fold higher as compared to activity with 3-cyanopyridine Pannonibacter carbonis thiophene-3-carboxylic acid + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 PaCNit
-
Pannonibacter carbonis
3.5.5.7 PaCNit
-
Pannonibacter carbonis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 65
-
-
Pannonibacter carbonis
3.5.5.7 65
-
-
Pannonibacter carbonis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 30 90 30°C: about 60% of maximal activity, 90°C: about 50% of maximal activity Pannonibacter carbonis
3.5.5.7 30 90 30°C: about 60% of maximal activity, 90°C: about 45% of maximal activity Pannonibacter carbonis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.7 50
-
48 h, 35% loss of activity Pannonibacter carbonis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7
-
-
Pannonibacter carbonis
3.5.5.7 7
-
-
Pannonibacter carbonis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 4 11 pH 4.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity Pannonibacter carbonis
3.5.5.7 3 11 pH 3.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity Pannonibacter carbonis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.5.1 6 7 relative enzyme activity is retained at pH 6.0 and 7.0 after incubating for 12 h at 4°C Pannonibacter carbonis