EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | acetone | 5% (v/v), 1.2fold activation | Pannonibacter carbonis | |
3.5.5.1 | dithiothreitol | 1 mM, 1.2fold activation | Pannonibacter carbonis | |
3.5.5.1 | EDTA | 1 mM, 1.35fold activation | Pannonibacter carbonis | |
3.5.5.1 | glycerin | 1% (v/v), 1.3fold activation | Pannonibacter carbonis | |
3.5.5.1 | L-cysteine | 1 mM, 1.3fold activation | Pannonibacter carbonis | |
3.5.5.1 | L-glutathione | 1 mM, 1.3fold activation | Pannonibacter carbonis | |
3.5.5.1 | SDS | 1% (v/v), 1.4fold activation | Pannonibacter carbonis | |
3.5.5.1 | Span-80 | 1% (v/v), 1.2fold activation | Pannonibacter carbonis | |
3.5.5.7 | DTT | 1 mM, 1.18fold activation | Pannonibacter carbonis | |
3.5.5.7 | EDTA | 1 mM, 1.35fold activation | Pannonibacter carbonis | |
3.5.5.7 | glycerin | 1% (v/v), 1.3fold activation | Pannonibacter carbonis | |
3.5.5.7 | Isopropanol | 1% (v/v), 1.2fold activation | Pannonibacter carbonis | |
3.5.5.7 | L-cysteine | 1 mM, 1.29fold activation | Pannonibacter carbonis | |
3.5.5.7 | L-glutathione | 1 mM, 1.27fold activation | Pannonibacter carbonis | |
3.5.5.7 | n-hexane | 1% (v/v), 1.1fold activation | Pannonibacter carbonis | |
3.5.5.7 | SDS | 1% (v/v), 1.4fold activation | Pannonibacter carbonis | |
3.5.5.7 | Span-80 | 1% (v/v), 1.2fold activation | Pannonibacter carbonis |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.5.1 | synthesis | the broad range of substrate specificity, the high activity towards aliphatic, aromatic, and arylacetonitriles, and the highly stability, in terms of temperature, pH, and metal ions make the enzyme a promising biocatalyst for mild nitrile hydrolysis | Pannonibacter carbonis |
3.5.5.7 | industry | the enzyme is a promising biocatalyst for mild nitrile hydrolysis | Pannonibacter carbonis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.5.1 | expression in Escherichia coli BL21 (DE3) | Pannonibacter carbonis |
3.5.5.7 | expression in Escherichia coli | Pannonibacter carbonis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | BaCl2 | 5 mM, 35% inhibition | Pannonibacter carbonis | |
3.5.5.1 | CaCl2 | 5 mM, 34% inhibition | Pannonibacter carbonis | |
3.5.5.1 | dimethyl sulfoxide | 1% (v/v) | Pannonibacter carbonis | |
3.5.5.1 | dithiothreitol | 5 mM, 37% inhibition | Pannonibacter carbonis | |
3.5.5.1 | DMSO | 5% (v/v), % inhibition | Pannonibacter carbonis | |
3.5.5.1 | ether | 5% (v/v), 23% inhibition | Pannonibacter carbonis | |
3.5.5.1 | ethyl acetate | 5% (v/v), 35% inhibition | Pannonibacter carbonis | |
3.5.5.1 | FeCl3 | 5 mM, 23% inhibition | Pannonibacter carbonis | |
3.5.5.1 | FeSO4 | 5 mM, 47% inhibition | Pannonibacter carbonis | |
3.5.5.1 | glycerin | 5% (v/v), 52% inhibition | Pannonibacter carbonis | |
3.5.5.1 | KCl | 5 mM, 58% inhibition | Pannonibacter carbonis | |
3.5.5.1 | methanol | 5% (v/v), 29% inhibition | Pannonibacter carbonis | |
3.5.5.1 | MgCl2 | 5 mM, 18% inhibition | Pannonibacter carbonis | |
3.5.5.1 | n-heptane | 5% (v/v), 25% inhibition | Pannonibacter carbonis | |
3.5.5.1 | NaCl | 5 mM, 26% inhibition | Pannonibacter carbonis | |
3.5.5.1 | SDS | 5% (v/v), 37% inhibition | Pannonibacter carbonis | |
3.5.5.1 | Triton X-100 | 5% (v/v), 14% inhibition | Pannonibacter carbonis | |
3.5.5.1 | ZnCl2 | 5 mM, 32% inhibition | Pannonibacter carbonis | |
3.5.5.7 | BaCl2 | 5 mM, 35% inhibition | Pannonibacter carbonis | |
3.5.5.7 | CaCl2 | 5 mM, 34% inhibition | Pannonibacter carbonis | |
3.5.5.7 | DTT | 5 mM, 37% inhibition | Pannonibacter carbonis | |
3.5.5.7 | FeCl3 | 5 mM, 23% inhibition | Pannonibacter carbonis | |
3.5.5.7 | FeSO4 | 5 mM, 47% inhibition | Pannonibacter carbonis | |
3.5.5.7 | glycerin | 5% (v/v), 52% inhibition | Pannonibacter carbonis | |
3.5.5.7 | KCl | 5 mM, 58% inhibition | Pannonibacter carbonis | |
3.5.5.7 | MgCl2 | 5 mM, 18% inhibition | Pannonibacter carbonis | |
3.5.5.7 | MnCl2 | 5 mM, 50% inhibition | Pannonibacter carbonis | |
3.5.5.7 | n-hexane | 5% (v/v), 6% inhibition | Pannonibacter carbonis | |
3.5.5.7 | NaCl | 5 mM, 26% inhibition | Pannonibacter carbonis | |
3.5.5.7 | SDS | 5% (v/v), 37% inhibition | Pannonibacter carbonis | |
3.5.5.7 | Triton X-100 | 5% (v/v), 14% inhibition | Pannonibacter carbonis | |
3.5.5.7 | ZnCl2 | 5 mM, 32% inhibition | Pannonibacter carbonis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.1 | 10.31 | - |
3-Cyanopyridine | pH 7.0, 65°C | Pannonibacter carbonis | |
3.5.5.7 | 10.31 | - |
3-Cyanopyridine | pH 7.2, 30°C | Pannonibacter carbonis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.1 | Ag+ | 1 mM, 3.2fold activation | Pannonibacter carbonis | |
3.5.5.1 | Cr3+ | 5 mM, 2.8fold activation | Pannonibacter carbonis | |
3.5.5.1 | Cu2+ | 5 mM, 1.3fold activation | Pannonibacter carbonis | |
3.5.5.1 | Hg2+ | 5 mM, 5.2fold activation | Pannonibacter carbonis | |
3.5.5.1 | Ni2+ | 5 mM, 3fold activation | Pannonibacter carbonis | |
3.5.5.7 | AgNO3 | 1 mM, 1.97fold activation | Pannonibacter carbonis | |
3.5.5.7 | CoCl2 | 1 mM, 1.08fold activation | Pannonibacter carbonis | |
3.5.5.7 | CuCl2 | 1 mM, 1.28fold activation | Pannonibacter carbonis | |
3.5.5.7 | HgCl2 | 1 mM, 3.2fold activation | Pannonibacter carbonis | |
3.5.5.7 | NiCl2 | 1 mM, 1.67fold activation | Pannonibacter carbonis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 31000 | - |
SDS-PAGE | Pannonibacter carbonis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.1 | Pannonibacter carbonis | - |
- |
- |
3.5.5.1 | Pannonibacter carbonis Q4.6 | - |
- |
- |
3.5.5.7 | Pannonibacter carbonis | - |
- |
- |
3.5.5.7 | Pannonibacter carbonis Q4.6 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.1 | - |
Pannonibacter carbonis |
3.5.5.7 | - |
Pannonibacter carbonis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.1 | 2-methylglutaronitrile + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
3.5.5.1 | 3-cyanopyridine + 2 H2O | - |
Pannonibacter carbonis | nicotinic acid + NH3 | - |
? | |
3.5.5.1 | 4-chlorobutyronitrile + H2O | - |
Pannonibacter carbonis | 4-chlorobutyrate + NH3 | - |
? | |
3.5.5.1 | acetonitrile + H2O | - |
Pannonibacter carbonis | acetic acid + NH3 | - |
? | |
3.5.5.1 | acrylonitrile + H2O | - |
Pannonibacter carbonis | acrylic acid + NH3 | - |
? | |
3.5.5.1 | benzonitrile + 2 H2O | - |
Pannonibacter carbonis | benzoic acid + NH3 | - |
? | |
3.5.5.1 | dicyanobutane + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
3.5.5.1 | dicyanooctane + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
3.5.5.1 | dodecanenitrile + H2O | - |
Pannonibacter carbonis | dodecanoic acid + NH3 | - |
? | |
3.5.5.1 | fumaronitrile + H2O | - |
Pannonibacter carbonis | fumaric acid + NH3 | - |
? | |
3.5.5.1 | iminodiacetonitrile + H2O | - |
Pannonibacter carbonis | iminodiacetic acid + NH3 | - |
? | |
3.5.5.1 | indole-3-acetonitrile + H2O | - |
Pannonibacter carbonis | indol-3-acetic acid + NH3 | - |
? | |
3.5.5.1 | mandelonitrile + H2O | - |
Pannonibacter carbonis | mandelic acid + NH3 | - |
? | |
3.5.5.1 | pentanenitrile + H2O | - |
Pannonibacter carbonis | pentanoic acid + NH3 | - |
? | |
3.5.5.1 | phenylacetonitrile + 2 H2O | - |
Pannonibacter carbonis | phenylacetic acid + NH3 | - |
? | |
3.5.5.1 | phenylenediacetonitrile + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
3.5.5.1 | phenylpropionitrile + 2 H2O | - |
Pannonibacter carbonis | phenylpropionic acid + NH3 | - |
? | |
3.5.5.1 | succinonitrile + H2O | - |
Pannonibacter carbonis | succinic acid + NH3 | - |
? | |
3.5.5.1 | thiophene + H2O | - |
Pannonibacter carbonis | ? + NH3 | - |
? | |
3.5.5.7 | 2-methylglutaronitrile + 4 H2O | activity is 2.3fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | 2-methylglutaric acid + 2 NH3 | - |
? | |
3.5.5.7 | 2-methylglutaronitrile + 4 H2O | activity is 2.3fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis Q4.6 | 2-methylglutaric acid + 2 NH3 | - |
? | |
3.5.5.7 | 3-cyanopyridine + 2 H2O | - |
Pannonibacter carbonis | pyridine 3-carboxylic acid + NH3 | - |
? | |
3.5.5.7 | 3-cyanopyridine + 2 H2O | - |
Pannonibacter carbonis Q4.6 | pyridine 3-carboxylic acid + NH3 | - |
? | |
3.5.5.7 | 3-phenylpropionitrile + 2 H2O | activity is 2.15fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | 3-phenylpropionic acid + NH3 | - |
? | |
3.5.5.7 | 4-chlorobutyronitrile + 2 H2O | activity is 2.44fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | 4-chlorobutanoic acid + NH3 | - |
? | |
3.5.5.7 | acrylonitrile + 2 H2O | activity is 2.2fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | acrylic acid + NH3 | - |
? | |
3.5.5.7 | acrylonitrile + 2 H2O | activity is 2.2fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis Q4.6 | acrylic acid + NH3 | - |
? | |
3.5.5.7 | adiponitrile + 4 H2O | activity is 2.13fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | adipic acid + 2 NH3 | - |
? | |
3.5.5.7 | benzonitrile + 2 H2O | activity is 2.24fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | benzoic acid + NH3 | - |
? | |
3.5.5.7 | benzonitrile + 2 H2O | activity is 2.24fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis Q4.6 | benzoic acid + NH3 | - |
? | |
3.5.5.7 | dodecanenitrile + 2 H2O | activity is 2.46fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | dodecanoic acid + NH3 | - |
? | |
3.5.5.7 | fumaronitrile + 4 H2O | activity is 2.21fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | fumaric acid + 2 NH3 | - |
? | |
3.5.5.7 | iminodiacetonitrile + 2 H2O | activity is 2.34fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | iminodiacetic acid + NH3 | - |
? | |
3.5.5.7 | additional information | the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles | Pannonibacter carbonis | ? | - |
- |
|
3.5.5.7 | additional information | the enzyme shows broad substrate specificity towards aliphatic, aromatic, and heterocyclic nitriles | Pannonibacter carbonis Q4.6 | ? | - |
- |
|
3.5.5.7 | pentanenitrile + 2 H2O | activity is 2.39fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | pentanoic acid + NH3 | - |
? | |
3.5.5.7 | sebaconitrile + 4 H2O | activity is 1.92fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | sebaconic acid + 2 NH3 | - |
? | |
3.5.5.7 | succinonitrile + 4 H2O | activity is 2.64fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | succinic acid + 2 NH3 | - |
? | |
3.5.5.7 | thiophene-3-carbonitrile + 2 H2O | activity is 2.42fold higher as compared to activity with 3-cyanopyridine | Pannonibacter carbonis | thiophene-3-carboxylic acid + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.5.1 | PaCNit | - |
Pannonibacter carbonis |
3.5.5.7 | PaCNit | - |
Pannonibacter carbonis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 65 | - |
- |
Pannonibacter carbonis |
3.5.5.7 | 65 | - |
- |
Pannonibacter carbonis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 30 | 90 | 30°C: about 60% of maximal activity, 90°C: about 50% of maximal activity | Pannonibacter carbonis |
3.5.5.7 | 30 | 90 | 30°C: about 60% of maximal activity, 90°C: about 45% of maximal activity | Pannonibacter carbonis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 50 | - |
48 h, 35% loss of activity | Pannonibacter carbonis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 7 | - |
- |
Pannonibacter carbonis |
3.5.5.7 | 7 | - |
- |
Pannonibacter carbonis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 4 | 11 | pH 4.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity | Pannonibacter carbonis |
3.5.5.7 | 3 | 11 | pH 3.0: about 40% of maximal activity, pH 11.0: about 50% of maximal activity | Pannonibacter carbonis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 6 | 7 | relative enzyme activity is retained at pH 6.0 and 7.0 after incubating for 12 h at 4°C | Pannonibacter carbonis |