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Literature summary extracted from

  • Sun, H.; Gao, W.; Wang, H.; Wei, D.
    Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S-selectivity toward mandelonitrile present in active inclusion bodies (2016), Biotechnol. Lett., 38, 455-461 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.5 overexpression in Escherichia coli BL21 (DE3) and formation ofd inclusion bodies that are active toward mandelonitrile and stable across a broad range of temperature and pH Pseudomonas psychrotolerans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.5 37000
-
 SDS-PAGE Pseudomonas psychrotolerans

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Pseudomonas psychrotolerans
-
-
-
3.5.5.5 Pseudomonas psychrotolerans L19
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 (R,S)-mandelonitrile + 2 H2O the enzyme hydrolyzes racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess value of 52.7%. No byproduct is observed Pseudomonas psychrotolerans (S)-mandelic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O activity is 4.2% compared to activity with mandelonitrile Pseudomonas psychrotolerans 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O activity is 4.2% compared to activity with mandelonitrile Pseudomonas psychrotolerans L19 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 2-chloromandelonitrile + H2O activity is 20% compared to activity with mandelonitrile Pseudomonas psychrotolerans 2-chloromandelic acid + NH3
-
 ?
3.5.5.5 2-cyanopyridine + 2 H2O activity is 28% compared to activity with mandelonitrile Pseudomonas psychrotolerans pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.5 2-cyanopyridine + 2 H2O activity is 28% compared to activity with mandelonitrile Pseudomonas psychrotolerans L19 pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.5 2-methylglutaronitrile + H2O activity is 9.6% compared to activity with mandelonitrile Pseudomonas psychrotolerans 2-methylglutarate + NH3
-
 ?
3.5.5.5 3-cyanopyridine + H2O activity is 29% compared to activity with mandelonitrile Pseudomonas psychrotolerans pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.5 3-hydroxyphenylpropionitrile + H2O activity is 49% compared to activity with mandelonitrile Pseudomonas psychrotolerans 3-hydroxyphenylpropionic acid + NH3
-
 ?
3.5.5.5 alpha-methylphenylacetonitrile + H2O activity is 88% compared to activity with mandelonitrile Pseudomonas psychrotolerans alpha-methylphenylacetic acid + NH3
-
 ?
3.5.5.5 benzonitrile + 2 H2O activity is 64% compared to activity with mandelonitrile Pseudomonas psychrotolerans benzoate + NH3
-
 ?
3.5.5.5 benzonitrile + 2 H2O activity is 64% compared to activity with mandelonitrile Pseudomonas psychrotolerans L19 benzoate + NH3
-
 ?
3.5.5.5 additional information activity of less than 1% compared to the activity with madelonitrile: fumaronitrile, 3-hydroxyglutaronitrile, succinonitrile, 3-hydroxypropionitrile. The enzyme hydrolyzes nitriles with diverse structures. Arylacetonitriles are the optimal substrates Pseudomonas psychrotolerans ?
-
-
3.5.5.5 additional information activity of less than 1% compared to the activity with madelonitrile: fumaronitrile, 3-hydroxyglutaronitrile, succinonitrile, 3-hydroxypropionitrile. The enzyme hydrolyzes nitriles with diverse structures. Arylacetonitriles are the optimal substrates Pseudomonas psychrotolerans L19 ?
-
-
3.5.5.5 phenylacetonitrile + 2 H2O activity is 404% compared to activity with mandelonitrile Pseudomonas psychrotolerans phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O activity is 404% compared to activity with mandelonitrile Pseudomonas psychrotolerans L19 phenylacetate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.5 ? x * 37000, SDS-PAGE Pseudomonas psychrotolerans

Synonyms

EC Number Synonyms Comment Organism
3.5.5.5 nitrilase PpL19
-
 Pseudomonas psychrotolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.5 30
-
-
 Pseudomonas psychrotolerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.5 25 60 25°C: about 85% of maximal activity, 60°C: about 80% of maximal activity Pseudomonas psychrotolerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.5 20
-
 40 h, about 50% loss of activity Pseudomonas psychrotolerans
3.5.5.5 30
-
 10 h, about 50% loss of activity Pseudomonas psychrotolerans
3.5.5.5 40
-
 3 h, about 50% loss of activity Pseudomonas psychrotolerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.5 8
-
-
 Pseudomonas psychrotolerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.5 6.5 10 pH 6.5: about 65% of maximal activity, pH 10.0: about 70% of maximal activity Pseudomonas psychrotolerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.5.5 7.6
-
 35 h, about 50% loss of activity Pseudomonas psychrotolerans
3.5.5.5 8
-
 12 h, about 50% loss of activity Pseudomonas psychrotolerans
3.5.5.5 8.6
-
 3 h, about 50% loss of activity Pseudomonas psychrotolerans