EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.28 | synthesis | engineering of a thermostable chloramphenicol acetyltransferase for enhanced isobutyl acetate production at elevated temperatures. Mutant F97W significantly increases conversion of isobutanol to isobutyl acetate. Using F97W, direct conversion of cellulose into isobutyl acetate is possible by an engineered Clostridium thermocellum strain at elevated temperatures | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.28 | homology modeling of structure. The binding pocket consists of Tyr20, Phe27, Tyr50, Thr88, Ile89, Phe90, Phe97, Ser140, Leu141, Ser142, Ile143, Ile144, Pro145, Trp146, Phe152, Leu154, Ile166, Ile167, Thr168, His189, Asp193, Gly194, and Tyr195, where His189 and Asp193 are the catalytic sites | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.28 | 0.18 | - |
chloramphenicol | mutant F97W, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 0.28 | - |
chloramphenicol | wild-type, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 138.7 | - |
isobutanol | wild-type, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 144.8 | - |
isobutanol | mutant F97W, pH 8.0, 50°C | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.28 | Staphylococcus aureus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.28 | acetyl-CoA + 2-phenylethanol | - |
Staphylococcus aureus | CoA + 2-phenylethyl acetate | - |
? | |
2.3.1.28 | acetyl-CoA + benzyl alcohol | - |
Staphylococcus aureus | CoA + benzyl acetate | - |
? | |
2.3.1.28 | acetyl-CoA + chloramphenicol | - |
Staphylococcus aureus | CoA + chloramphenicol 3-acetate | - |
? | |
2.3.1.28 | acetyl-CoA + isobutanol | - |
Staphylococcus aureus | CoA + isobutyl acetate | - |
? | |
2.3.1.28 | additional information | poor substrates: butanol, propanol, ethanol, isopropanol. Specific ester production titers and rates are higher for aromatic alcohols than linear, short-chain alcohols | Staphylococcus aureus | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.28 | ? | x * 25800, SDS-PAGE | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.28 | CAT | - |
Staphylococcus aureus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.28 | 68.3 | - |
melting temperature, mutant F97W | Staphylococcus aureus |
2.3.1.28 | 72 | - |
melting temperature, wild-type | Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.28 | 0.3 | - |
isobutanol | wild-type, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 0.6 | - |
isobutanol | mutant F97W, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 102.6 | - |
chloramphenicol | mutant F97W, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 203 | - |
chloramphenicol | wild-type, pH 8.0, 50°C | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.28 | 0.0022 | - |
isobutanol | wild-type, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 0.0041 | - |
isobutanol | mutant F97W, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 577 | - |
chloramphenicol | mutant F97W, pH 8.0, 50°C | Staphylococcus aureus | |
2.3.1.28 | 737 | - |
chloramphenicol | wild-type, pH 8.0, 50°C | Staphylococcus aureus |