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Literature summary extracted from

  • Liu, N.; Fosses, A.; Kampik, C.; Parsiegla, G.; Denis, Y.; Vita, N.; Fierobe, H.P.; Perret, S.
    In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum implication for cellulose catabolism (2019), Biotechnol. Biofuels, 12, 208 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.20 expressed in Escherichia coli BL21(DE3) cells Ruminiclostridium cellulolyticum
2.4.1.49 expression in Escherichia coli Ruminiclostridium cellulolyticum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.49 molecular modeling of structure Ruminiclostridium cellulolyticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.49 4.7
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 5.4
-
 cellotriose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 5.6
-
 cellopentaose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 11.9
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Ruminiclostridium cellulolyticum
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate Ruminiclostridium cellulolyticum ATCC 35319
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Ruminiclostridium cellulolyticum
-
-
-
2.4.1.20 Ruminiclostridium cellulolyticum ATCC 35319
-
-
-
2.4.1.49 Ruminiclostridium cellulolyticum B8I1R5 isoform CbpB
-
2.4.1.49 Ruminiclostridium cellulolyticum B8I1W7 isoform CbpA
-
2.4.1.49 Ruminiclostridium cellulolyticum B8I5F2 isoform CbpB
-
2.4.1.49 Ruminiclostridium cellulolyticum DSM 5812 B8I1R5 isoform CbpB
-
2.4.1.49 Ruminiclostridium cellulolyticum DSM 5812 B8I1W7 isoform CbpA
-
2.4.1.49 Ruminiclostridium cellulolyticum DSM 5812 B8I5F2 isoform CbpB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20 Ni-NTA resin column chromatography and Mono Q column chromatography Ruminiclostridium cellulolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 cellobiose + phosphate
-
 Ruminiclostridium cellulolyticum alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Ruminiclostridium cellulolyticum ATCC 35319 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.49 cellopentaose + phosphate
-
 Ruminiclostridium cellulolyticum cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellopentaose + phosphate poor substrate Ruminiclostridium cellulolyticum cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellopentaose + phosphate poor substrate Ruminiclostridium cellulolyticum DSM 5812 cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellopentaose + phosphate
-
 Ruminiclostridium cellulolyticum DSM 5812 cellotetraose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotetraose + phosphate
-
 Ruminiclostridium cellulolyticum cellotriose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotetraose + phosphate poor substrate Ruminiclostridium cellulolyticum cellotriose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotetraose + phosphate poor substrate Ruminiclostridium cellulolyticum DSM 5812 cellotriose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotetraose + phosphate
-
 Ruminiclostridium cellulolyticum DSM 5812 cellotriose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotriose + phosphate
-
 Ruminiclostridium cellulolyticum cellobiose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotriose + phosphate poor substrate Ruminiclostridium cellulolyticum cellobiose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotriose + phosphate
-
 Ruminiclostridium cellulolyticum DSM 5812 cellobiose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.49 cellotriose + phosphate poor substrate Ruminiclostridium cellulolyticum DSM 5812 cellobiose + alpha-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.20 ? x * 93500, calculated from amino acid sequence Ruminiclostridium cellulolyticum
2.4.1.49 ? x * 90500, SDS-PAGE and calculated from sequence Ruminiclostridium cellulolyticum
2.4.1.49 ? x * 91000, SDS-PAGE and calculated from sequence Ruminiclostridium cellulolyticum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.20 CbpA
-
 Ruminiclostridium cellulolyticum
2.4.1.20 cellobiose phosphorylase A
-
 Ruminiclostridium cellulolyticum
2.4.1.49 CbpA
-
 Ruminiclostridium cellulolyticum
2.4.1.49 Ccel_1439
-
 Ruminiclostridium cellulolyticum
2.4.1.49 Ccel_2354
-
 Ruminiclostridium cellulolyticum
2.4.1.49 Ccel_3412
-
 Ruminiclostridium cellulolyticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.49 1
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 39
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 47.8
-
 cellopentaose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 127.5
-
 cellotriose pH 7, 37°C Ruminiclostridium cellulolyticum

Expression

EC Number Organism Comment Expression
2.4.1.20 Ruminiclostridium cellulolyticum enzyme expression is induced during cellulose growth up
2.4.1.49 Ruminiclostridium cellulolyticum only the expression of the gene encoding the cellobiose phosphorylase and the cellodextrin phosphorlyase isoform CdpA is induced during cellulose growth additional information

General Information

EC Number General Information Comment Organism
2.4.1.20 metabolism the enzyme is essential for growth on cellulose Ruminiclostridium cellulolyticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.49 0.21
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 3.29
-
 cellotetraose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 8.59
-
 cellopentaose pH 7, 37°C Ruminiclostridium cellulolyticum
2.4.1.49 23.5
-
 cellotriose pH 7, 37°C Ruminiclostridium cellulolyticum