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Literature summary extracted from

  • Zhang, N.; Mao, Z.; Luo, L.; Wan, X.; Huang, F.; Gong, Y.
    Two bifunctional enzymes from the marine protist Thraustochytrium roseum biochemical characterization of wax ester synthase/acyl-CoA diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis (2017), Biotechnol. Biofuels, 10, 185 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.75 NaCl isoform WSD4 shows maximum enzyme activity at NaCl concentration of 400 mM Thraustochytrium roseum
2.3.1.75 NaCl isoform WSD5 shows maximum enzyme activity at NaCl concentration of 800 mM Thraustochytrium roseum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast Thraustochytrium roseum
2.3.1.20 gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast Thraustochytrium roseum
2.3.1.75 expressed in Escherichia coli Rosetta (DE3) cells and Saccharomyces cerevisiae quadruple mutant H1246 Thraustochytrium roseum
2.3.1.75 gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast Thraustochytrium roseum
2.3.1.75 gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast Thraustochytrium roseum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 oleoyl-CoA substrate inhibition; substrate inhibition Thraustochytrium roseum
2.3.1.75 oleoyl-CoA substrate inhibition; substrate inhibition Thraustochytrium roseum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.20 additional information
-
 additional information kinetics of wax synthase activity, overview Thraustochytrium roseum
2.3.1.75 additional information
-
 additional information kinetics of wax synthase activity, overview Thraustochytrium roseum
2.3.1.75 0.00014
-
 lauroyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.00014
-
 lauroyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.00096
-
 decanoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.001
-
 decanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.00308
-
 palmitoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.00308
-
 palmitoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.00394
-
 myristoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.00394
-
 myristoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.01225
-
 octanoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0123
-
 octanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0176
-
 decanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0176
-
 decanoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0185
-
 myristoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0185
-
 myristoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.01915
-
 stearoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.01915
-
 stearoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.02477
-
 palmitoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0248
-
 palmitoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0259
-
 lauroyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.02593
-
 lauroyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.02993
-
 octanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.02993
-
 octanoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.07587
-
 stearoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0759
-
 stearoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.10162
-
 eicosapentaenoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.1062
-
 eicosapentaenoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.13116
-
 eicosapentaenoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.1312
-
 eicosapentaenoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.22635
-
 Arachidonoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.2264
-
 Arachidonoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.28086
-
 octadecatrienoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.2809
-
 octadecatrienoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.28468
-
 octadecatrienoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.2847
-
 octadecatrienoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.29717
-
 Arachidonoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.2972
-
 Arachidonoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Thraustochytrium roseum
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Thraustochytrium roseum ATCC28210
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.75 acyl-CoA + a long-chain alcohol Thraustochytrium roseum
-
 CoA + a long-chain ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Thraustochytrium roseum A0A1Z2RWY4
-
-
2.3.1.20 Thraustochytrium roseum A0A3G1ICG7
-
-
2.3.1.75 Thraustochytrium roseum A0A1Z2RWY4
-
-
2.3.1.75 Thraustochytrium roseum A0A3G1ICG7
-
-
2.3.1.75 Thraustochytrium roseum ATCC28210 A0A1Z2RWY4
-
-
2.3.1.75 Thraustochytrium roseum ATCC28210 A0A3G1ICG7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Thraustochytrium roseum
2.3.1.75 Ni2+-affinity column chromatography Thraustochytrium roseum
2.3.1.75 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Thraustochytrium roseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thraustochytrium roseum CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thraustochytrium roseum ATCC28210 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity of wax synthase activity, overview Thraustochytrium roseum ?
-
-
2.3.1.20 additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity of wax synthase activity, overview Thraustochytrium roseum ATCC28210 ?
-
-
2.3.1.20 palmitoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thraustochytrium roseum CoA + 1,2-diacyl-3-palmitoylglycerol
-
 ?
2.3.1.20 palmitoyl-CoA + 1,2-diacyl-sn-glycerol
-
 Thraustochytrium roseum ATCC28210 CoA + 1,2-diacyl-3-palmitoylglycerol
-
 ?
2.3.1.75 acyl-CoA + a long-chain alcohol
-
 Thraustochytrium roseum CoA + a long-chain ester
-
 ?
2.3.1.75 acyl-CoA + decanol
-
 Thraustochytrium roseum CoA + decanyl acylester
-
 ?
2.3.1.75 acyl-CoA + dodecanol
-
 Thraustochytrium roseum CoA + dodecanyl acylester
-
 ?
2.3.1.75 acyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecanyl acylester
-
 ?
2.3.1.75 acyl-CoA + octadecanol
-
 Thraustochytrium roseum CoA + octadecanyl acylester
-
 ?
2.3.1.75 arachidonoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum CoA + hexadecyl arachidonoate
-
 ?
2.3.1.75 arachidonoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl arachidonoate
-
 ?
2.3.1.75 decanoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 decanoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl decanoate
-
 ?
2.3.1.75 eicosapentaenoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 eicosapentaenoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl eicosapentaenoate
-
 ?
2.3.1.75 lauroyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 lauroyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl laurate
-
 ?
2.3.1.75 lauroyl-CoA + hexadecanol best acyl-CoA substrate Thraustochytrium roseum CoA + hexadecyl laurate
-
 ?
2.3.1.75 additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity, overview. TrWSD4 accepts four linear alcohols as substrates and the highest WS activity is observed for octadecanol (C18:0-OH), which is 2fold higher than the activity using decanol as substrate (C10:0-OH) Thraustochytrium roseum ?
-
-
2.3.1.75 additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity, overview. TrWSD5 accepts four linear alcohols as substrates and the highest WS activity is observed for octadecanol (C18:0-OH), which is 2fold higher than the activity using decanol as substrate (C10:0-OH) Thraustochytrium roseum ?
-
-
2.3.1.75 additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity, overview. TrWSD4 accepts four linear alcohols as substrates and the highest WS activity is observed for octadecanol (C18:0-OH), which is 2fold higher than the activity using decanol as substrate (C10:0-OH) Thraustochytrium roseum ATCC28210 ?
-
-
2.3.1.75 additional information the recombinant protein purified from Escherichia coli strain Rosetta (DE3) has substantial wax synthase (WS) and lower diacylglycerol acyltransferase (DGAT) activity. Acyl-CoA substrate specificity, overview. TrWSD5 accepts four linear alcohols as substrates and the highest WS activity is observed for octadecanol (C18:0-OH), which is 2fold higher than the activity using decanol as substrate (C10:0-OH) Thraustochytrium roseum ATCC28210 ?
-
-
2.3.1.75 myristoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 myristoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl myristate
-
 ?
2.3.1.75 myristoyl-CoA + hexadecanol preferred acyl-CoA substrate Thraustochytrium roseum CoA + hexadecyl myristate
-
 ?
2.3.1.75 octadecatrienoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 octadecatrienoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl octadecatrienoate
-
 ?
2.3.1.75 octanoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 octanoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl octanoate
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum CoA + hexadecyl palmitate
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + hexadecyl palmitate
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecanol
-
 Thraustochytrium roseum ATCC28210 CoA + hexadecyl palmitate
-
 ?
2.3.1.75 stearoyl-CoA + hexadecan-1-ol
-
 Thraustochytrium roseum ?
-
 ?
2.3.1.75 stearoyl-CoA + hexadecanol
-
 Thraustochytrium roseum CoA + stearoyl hexadecanate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.75 ? x * 100000, His6-tagged isoform WSD5, SDS-PAGE Thraustochytrium roseum
2.3.1.75 ? x * 80000, His6-tagged isoform WSD4, SDS-PAGE Thraustochytrium roseum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 bifunctional WS/DGAT
-
 Thraustochytrium roseum
2.3.1.20 More see also EC 2.3.1.75 Thraustochytrium roseum
2.3.1.20 TrWSD4
-
 Thraustochytrium roseum
2.3.1.20 TrWSD5
-
 Thraustochytrium roseum
2.3.1.20 wax ester synthase/acyl-CoA diacylglycerol acyltransferase
-
 Thraustochytrium roseum
2.3.1.20 wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
-
 Thraustochytrium roseum
2.3.1.20 WS/DGAT
-
 Thraustochytrium roseum
2.3.1.20 WSD4
-
 Thraustochytrium roseum
2.3.1.20 WSD5
-
 Thraustochytrium roseum
2.3.1.75 bifunctional WS/DGAT
-
 Thraustochytrium roseum
2.3.1.75 More see also EC 2.3.1.20 Thraustochytrium roseum
2.3.1.75 More see also EC 2.3.1.75 Thraustochytrium roseum
2.3.1.75 TrWSD4
-
 Thraustochytrium roseum
2.3.1.75 TrWSD5
-
 Thraustochytrium roseum
2.3.1.75 wax ester synthase/acyl-CoA diacylglycerol acyltransferase
-
 Thraustochytrium roseum
2.3.1.75 wax ester synthase/acyl-CoA:diacylglycerol acyltransferase
-
 Thraustochytrium roseum
2.3.1.75 WS/DGAT
-
 Thraustochytrium roseum
2.3.1.75 WS/DGAT bifunctional enzyme Thraustochytrium roseum
2.3.1.75 WSD4
-
 Thraustochytrium roseum
2.3.1.75 WSD4 isoform Thraustochytrium roseum
2.3.1.75 WSD5
-
 Thraustochytrium roseum
2.3.1.75 WSD5 isoform Thraustochytrium roseum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 37
-
 assay at Thraustochytrium roseum
2.3.1.75 37
-
-
 Thraustochytrium roseum
2.3.1.75 47
-
-
 Thraustochytrium roseum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.75 7 57 activity range, the WS activity of TrWSD4 is gradually increased when the temperature is increased from 7 to 47°C, while it decreases to 92% of the highest enzyme activity when the temperature reaches 57°C. Influence of increasing temperature on saturation curves using palmitoyl-CoA and hexadecanol as substrates, overview Thraustochytrium roseum
2.3.1.75 7 57 enzyme activity of Isoform WSD4 is gradually increased when the temperature is increased from 7 to 47°C while decrease to 92% of the highest enzyme activity when the temperature reaches 57°C Thraustochytrium roseum
2.3.1.75 7 57 the lowest WS activity of TrWSD5 is observed at the lowest and the highest temperatures tested in this assay. The enzyme activity of TrWSD5 appears to be stable when the temperature increases from 17 to 47°C. Influence of increasing temperature on saturation curves using palmitoyl-CoA and hexadecanol as substrates, overview Thraustochytrium roseum
2.3.1.75 17 47 the enzyme activity of isoform WSD5 is stable when the temperature increases from 17 to 47°C Thraustochytrium roseum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.75 0.00941
-
 decanoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0114
-
 eicosapentaenoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0114
-
 eicosapentaenoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0148
-
 Arachidonoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0148
-
 Arachidonoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0154
-
 decanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0154
-
 decanoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0185
-
 octanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0185
-
 octanoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0202
-
 lauroyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0202
-
 lauroyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0236
-
 octadecatrienoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0236
-
 octadecatrienoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0254
-
 myristoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0254
-
 myristoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.032
-
 palmitoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.032
-
 palmitoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0379
-
 stearoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.0379
-
 stearoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0941
-
 decanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.102
-
 octanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.102
-
 octanoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.165
-
 Arachidonoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.165
-
 Arachidonoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.242
-
 octadecatrienoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.242
-
 octadecatrienoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.243
-
 lauroyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.243
-
 lauroyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.331
-
 myristoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.331
-
 myristoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.341
-
 palmitoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.341
-
 palmitoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.498
-
 eicosapentaenoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.498
-
 eicosapentaenoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.604
-
 stearoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.604
-
 stearoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.4
-
 assay at Thraustochytrium roseum
2.3.1.75 7.4
-
 assay at Thraustochytrium roseum

General Information

EC Number General Information Comment Organism
2.3.1.20 evolution distinct evolutionary origin of Thraustochytrium roseum bifunctional wax ester synthase/acyl-CoA diacylglycerol acyltransferases (WS/DGATs) from that of plant and bacterial WS/DGATs. Both TrWSD4 and TrWSD5 contain the conserved acyltransferase active-site motif (HHXXXDG), whose first histidine residue is substituted to serine and aspartate, respectively Thraustochytrium roseum
2.3.1.20 physiological function two bifunctional enzymes, TrWSD4 and TrWSD5, from the marine protist Thraustochytrium roseum show wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis. The WS/DGAT shows both in vitro wax synthase (WS) and DGAT activity, it is characterized as unspecific acyltransferase accepting a broad range of acyl-CoAs and fatty alcohols as substrates for WS activity but displaying substrate preference for medium-chain acyl-CoAs. In vivo characterization shows that the WS/DGAT predominantly functions as wax synthase Thraustochytrium roseum
2.3.1.75 evolution distinct evolutionary origin of Thraustochytrium roseum bifunctional wax ester synthase/acyl-CoA diacylglycerol acyltransferases (WS/DGATs) from that of plant and bacterial WS/DGATs. Both TrWSD4 and TrWSD5 contain the conserved acyltransferase active-site motif (HHXXXDG), whose first histidine residue is substituted to serine and aspartate, respectively Thraustochytrium roseum
2.3.1.75 physiological function two bifunctional enzymes, TrWSD4 and TrWSD5, from the marine protist Thraustochytrium roseum show wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis. The WS/DGAT shows both in vitro WS and DGAT activity, it is characterized as unspecific acyltransferase accepting a broad range of acyl-CoAs and fatty alcohols as substrates for WS activity but displaying substrate preference for medium-chain acyl-CoAs. In vivo characterization shows that the WS/DGAT predominantly functions as wax synthase Thraustochytrium roseum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.75 0.0488
-
 Arachidonoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.05
-
 Arachidonoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.084
-
 octadecatrienoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.084
-
 octadecatrienoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.0866
-
 eicosapentaenoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.087
-
 eicosapentaenoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.618
-
 octanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.619
-
 octanoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.729
-
 Arachidonoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.73
-
 Arachidonoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.85
-
 octadecatrienoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 0.85
-
 octadecatrienoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.873
-
 decanoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 0.875
-
 decanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 1.98
-
 stearoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 1.98
-
 stearoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 4.69
-
 eicosapentaenoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 4.69
-
 eicosapentaenoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 6.44
-
 myristoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 6.45
-
 myristoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 7.96
-
 stearoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 7.96
-
 stearoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 8.33
-
 octanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 8.33
-
 octanoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 9.37
-
 lauroyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 9.37
-
 lauroyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 10.39
-
 palmitoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 10.4
-
 palmitoyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 13.7
-
 palmitoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 13.77
-
 palmitoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 17.89
-
 myristoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 17.9
-
 myristoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 98.02
-
 decanoyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 98.3
-
 decanoyl-CoA isoform WSD5, at pH 7.4 and 37°C Thraustochytrium roseum
2.3.1.75 144.29
-
 lauroyl-CoA pH 7.4, 37°C, recombinant enzyme Thraustochytrium roseum
2.3.1.75 146
-
 lauroyl-CoA isoform WSD4, at pH 7.4 and 37°C Thraustochytrium roseum