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Literature summary extracted from

  • Gao, Y.; Saburi, W.; Taguchi, Y.; Mori, H.
    Biochemical characteristics of maltose phosphorylase MalE from Bacillus sp. AHU2001 and chemoenzymatic synthesis of oligosaccharides by the enzyme (2019), Biosci. Biotechnol. Biochem., 83, 2097-2109 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.8 expression in Escherichia coli BL21 (DE3) Bacillus sp. AHU2001

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.8 phosphate
-
 Bacillus sp. AHU2001

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.8 0.295
-
 phosphate pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.835
-
 maltose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 5.68
-
 6-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 8.38
-
 D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 14.1
-
 1,5-anhydro-D-glucitol pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 15.1
-
 D-Lyxose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 15.6
-
 D-glucosamine pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 16.3
-
 methyl alpha-D-glucoside pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 19.2
-
 D-xylose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 20.7
-
 L-fucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 28.1
-
 L-sorbose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 31.2
-
 D-allose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 36.2
-
 kojibiose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 40.9
-
 D-mannose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 47.8
-
 2-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.8 90000
-
 SDS-PAGE Bacillus sp. AHU2001
2.4.1.8 193000
-
 gel filtration Bacillus sp. AHU2001

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.8 Bacillus sp. AHU2001
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.8 1,5-anhydro-D-glucitol + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 1,5-anhydro-4-O-alpha-D-glucopyranosyl-D-glucitol + phosphate
-
 ?
2.4.1.8 2-deoxy-D-glucose + beta-D-glucose 1-phosphate the enzyme synthesizes alpha-(1->3)-glucoside through reverse phosphorolysis with 2-deoxy-D-glucose Bacillus sp. AHU2001 2-deoxy-3-O-alpha-D-glucopyranosyl-D-arabino-hexopyranose + phosphate
-
 ?
2.4.1.8 3-deoxy-D-glucose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 3-deoxy-4-O-alpha-D-glucopyranosyl-D-ribo-hexopyranose + phosphate
-
 ?
2.4.1.8 6-deoxy-D-glucose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
 ?
2.4.1.8 D-allose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-allopyranose + phosphate
-
 ?
2.4.1.8 D-glucosamine + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 2-amino-2-deoxy-4-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
 ?
2.4.1.8 D-glucose + beta-D-glucose 1-phosphate
-
 Bacillus sp. AHU2001 maltose + phosphate
-
 ?
2.4.1.8 D-lyxose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-lyxopyranose + phosphate
-
 ?
2.4.1.8 D-mannose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-mannopyranose + phosphate
-
 ?
2.4.1.8 D-xylose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
-
 ?
2.4.1.8 kojibiose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 1,4-alpha-D-glucopyranosyl-[1,2-alpha-D-glucopyranosyl]-D-glucose + phosphate
-
 ?
2.4.1.8 L-fucose + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 4-O-alpha-D-glucopyranosyl-L-fucopyranose + phosphate
-
 ?
2.4.1.8 L-sorbose + beta-D-glucose 1-phosphate the enzyme synthesizes alpha-(1->3)-glucoside through reverse phosphorolysis with L-sorbose Bacillus sp. AHU2001 1,4-alpha-D-glucopyranosyl-[1,2-beta-D-glucopyranosyl]-D-glucose + phosphate
-
 ?
2.4.1.8 maltose + phosphate phosphorolytic activity to maltose, but not to other alpha-linked glucobioses and maltotriose. The phosphorolysis of maltose by MalE obeys the sequential bi-bi mechanism Bacillus sp. AHU2001 D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.8 methyl alpha-D-glucoside + beta-D-glucose 1-phosphate the enzyme synthesizes a alpha-(1->4)-glucoside Bacillus sp. AHU2001 methyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.8 homodimer 1 * 90000, SDS-PAGE Bacillus sp. AHU2001

Synonyms

EC Number Synonyms Comment Organism
2.4.1.8 MalE
-
 Bacillus sp. AHU2001

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.8 45
-
 phosphorolysis of maltose Bacillus sp. AHU2001

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.8 30 50 30°C: about 50% of maximal activity, 50°C: about 50% of maximal activity Bacillus sp. AHU2001

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.8 40
-
 residual activity is higher than 80% of the original activity at temperatures below 40°C after the heat treatment at pH 8.0 for 15 min Bacillus sp. AHU2001

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.8 0.436
-
 D-Lyxose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.568
-
 L-sorbose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.682
-
 D-allose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.957
-
 methyl alpha-D-glucoside pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 1.12
-
 1,5-anhydro-D-glucitol pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 1.21
-
 L-fucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 8.55
-
 D-mannose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 15.8
-
 kojibiose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 29.9
-
 D-xylose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 30.9
-
 maltose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 39.2
-
 2-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 69.1
-
 6-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 107
-
 D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 237
-
 D-glucosamine pH 8.0, 37°C Bacillus sp. AHU2001

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.8 8.1
-
 phosphorolysis of maltose Bacillus sp. AHU2001

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.8 6 8.5 pH 6.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity Bacillus sp. AHU2001

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.8 4.5 10.4 the residual activity is higher than 80% of the original activity at a pH range of 4.5-10.4 after the pH treatment at 4°C for 24 h Bacillus sp. AHU2001

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.8 1.74
-
 phosphate pH 8.0, 37°C Bacillus sp. AHU2001

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.8 0.0219
-
 D-allose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.0289
-
 D-Lyxose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.0585
-
 L-fucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.0687
-
 methyl alpha-D-glucoside pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.0701
-
 L-sorbose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.0784
-
 1,5-anhydro-D-glucitol pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.209
-
 D-mannose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.282
-
 3-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.436
-
 kojibiose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 0.82
-
 2-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 1.57
-
 D-xylose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 12.2
-
 6-deoxy-D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 12.8
-
 D-glucose pH 8.0, 37°C Bacillus sp. AHU2001
2.4.1.8 15.2
-
 D-glucosamine pH 8.0, 37°C Bacillus sp. AHU2001