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Literature summary extracted from

  • Guo, Y.; Zhao, P.; Zhang, W.; Li, X.; Chen, X.; Chen, D.
    Catalytic improvement and structural analysis of atrazine chlorohydrolase by site-saturation mutagenesis (2016), Biosci. Biotechnol. Biochem., 80, 1336-1343 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.8 expression in Escherichia coli BL21 (DE3) Haematococcus lacustris

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.8 D30G/G147D/G162E/A170V/H406P/V466A specific acitivity of the mutant enzyme is 3.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.0fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 D30G/I393P/L395S/N429S/V466A specific acitivity of the mutant enzyme is 3.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.2 fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 D30G/Q71G/M315I/R389C/H399Q/N429S/V466A specific acitivity of the mutant enzyme is 2.9fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S specific acitivity of the mutant enzyme is 2.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 D30G/Q91M/T195A/N429S/M99T/R290H specific acitivity of the mutant enzyme is 1.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 1.4fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 G55S/I393W/L395R/M315I/E433K specific acitivity of the mutant enzyme is 4.0fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.3 fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T specific acitivity of the mutant enzyme is 2.3fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.7fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 Q71R/V58A/R389C/I209M/E317G specific acitivity of the mutant enzyme is 2.4fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.9fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W specific acitivity of the mutant enzyme is 3.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.3fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12A/Q71A/M315I/M341V specific acitivity of the mutant enzyme is 5.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.9fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12A/Q71T/A173V/M315I/H399Q/N429S/V466A specific acitivity of the mutant enzyme is 2.5fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.7fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12A/Q71T/M315I/H399Q/N429S/V466A specific acitivity of the mutant enzyme is 2.4fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A specific acitivity of the mutant enzyme is 2.9fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.0fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12C/A139P/M315I/R389C/I393E/L395R/F439L specific acitivity of the mutant enzyme is 2.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 2.6fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12F/D30G/V58A/T121A/I393S/L395H specific acitivity of the mutant enzyme is fold 2.4higher than activity of wild-type enzyme. The kcat/Km-value is 2.7fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P specific acitivity of the mutant enzyme is 4.2fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.4fold higher than the wild-type value Haematococcus lacustris
3.8.1.8 V12T/I393G/R389C/L395W/N429S/L446S/V466A specific acitivity of the mutant enzyme is 3.4fold higher than activity of wild-type enzyme. The kcat/Km-value is 3.8fold higher than the wild-type value Haematococcus lacustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.8 0.09
-
Atrazine Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.11
-
Atrazine D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.131
-
Atrazine Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.132
-
Atrazine V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.138
-
Atrazine D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.14
-
Atrazine V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.142
-
Atrazine V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.145
-
Atrazine V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.148
-
Atrazine D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.15
-
Atrazine V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 0.15
-
Atrazine wild-type enzyme, pH 7.2, 23°C Haematococcus lacustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.8 atrazine + H2O Haematococcus lacustris
-
hydroxyatrazine + chloride
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.8 Haematococcus lacustris
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.8 3
-
substrate: atrazine, wild-type enzyme, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 3.6
-
substrate: atrazine, D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 6.8
-
substrate: atrazine, V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 6.9
-
substrate: atrazine, Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 7.1
-
substrate: atrazine, V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 7.3
-
substrate: atrazine, V12F/D30G/V58A/T121A/I393S/L395H, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 7.4
-
substrate: atrazine, Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 7.6
-
substrate: atrazine, D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 7.6
-
substrate: atrazine, V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 8.6
-
substrate: atrazine, D30G/Q71G/M315I/R389C/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 8.7
-
substrate: atrazine, V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 9.6
-
substrate: atrazine, D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 10.3
-
substrate: atrazine, V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 10.6
-
substrate: atrazine, D30G/I393P/L395S/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 10.7
-
substrate: atrazine, Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 12.2
-
substrate: atrazine, G55S/I393W/L395R/M315I/E433K, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 12.8
-
substrate: atrazine, V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 15.6
-
substrate: atrazine, V12A/Q71A/M315I/M341V, pH 7.2, 23°C Haematococcus lacustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.8 atrazine + H2O
-
Haematococcus lacustris hydroxyatrazine + chloride
-
?
3.8.1.8 atrazine + H2O
-
Haematococcus lacustris 2-hydroxyatrazine + chloride
-
?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.8 AtzA
-
Haematococcus lacustris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.8 1.66
-
Atrazine wild-type enzyme, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 1.67
-
Atrazine D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 2.87
-
Atrazine Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 3.81
-
Atrazine V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 3.86
-
Atrazine Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 4.06
-
Atrazine D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 4.28
-
Atrazine V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 4.32
-
Atrazine V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 4.73
-
Atrazine V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 4.87
-
Atrazine D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 5.86
-
Atrazine V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C Haematococcus lacustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.8 7.2
-
assay at Haematococcus lacustris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8.1.8 11.1
-
Atrazine wild-type enzyme, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 15.2
-
Atrazine D30G/Q91M/T195A/N429S/M99T/R290H, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 28.8
-
Atrazine V12A/Q71T/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 29
-
Atrazine V12C/A139P/M315I/R389C/I393E/L395R/F439L, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 29.1
-
Atrazine D30G/Q71P/R271C/L288Q/M337T/R389C/H399Q/N429S, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 29.2
-
Atrazine D30G/Q71G/M315I/R389C/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 29.4
-
Atrazine Q71P/D128Y/I194V/T195A/M315I/R389C/H399Q/A470T, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 29.5
-
Atrazine V12A/Q71T/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 30
-
Atrazine V12F/D30G/V58A/T121A/I393S/L395H, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 32
-
Atrazine Q71R/V58A/R389C/I209M/E317G, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 32.9
-
Atrazine D30G/G147D/G162E/A170V/H406P/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 33.2
-
Atrazine V12A/Q71T/T121A/A173V/M315I/H399Q/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 35.4
-
Atrazine D30G/I393P/L395S/N429S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 36.2
-
Atrazine Q7R/V12A/S307P/M337T/I393R/L395R/S438G/R462W, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 36.5
-
Atrazine G55S/I393W/L395R/M315I/E433K, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 37.7
-
Atrazine V12P/D30G/Q71R/H249Y/270V/R389C/I393P/L395P, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 42
-
Atrazine V12T/I393G/R389C/L395W/N429S/L446S/V466A, pH 7.2, 23°C Haematococcus lacustris
3.8.1.8 43.6
-
Atrazine V12A/Q71A/M315I/M341V, pH 7.2, 23°C Haematococcus lacustris