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Literature summary extracted from

  • Xue, Y.P.; Jiao, B.; Hua, D.E.; Cheng, F.; Liu, Z.Q.; Zheng, Y.G.
    Improving catalytic performance of an arylacetonitrilase by semirational engineering (2017), Bioprocess Biosyst. Eng., 40, 1565-1572 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 S190G mutant enzyme exhibits 3fold higher specific activity toward mandelonitrile compared with that of wild-type uncultured organism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 0.76
-
 mandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 1.98
-
 mandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 3.89
-
 2-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 3.98
-
 2-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 5.47
-
 3-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 5.67
-
 3-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 6.08
-
 4-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 6.15
-
 4-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 uncultured organism Q6RWE0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2-chloromandelonitrile + H2O
-
 uncultured organism 2-chloromandelic acid + NH3
-
 ?
3.5.5.1 3-chloromandelonitrile + H2O
-
 uncultured organism 3-chloromandelic acid + NH3
-
 ?
3.5.5.1 4-chloromandelonitrile + H2O
-
 uncultured organism 3-chloromandelic acid + NH3
-
 ?
3.5.5.1 mandelonitrile + H2O
-
 uncultured organism mandelic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 NitA
-
 uncultured organism

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.1 1.3
-
 2-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 1.82
-
 2-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 1.98
-
 3-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 2.2
-
 4-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 3.07
-
 4-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 3.41
-
 3-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 4.6
-
 mandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 13.23
-
 mandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.5.1 0.33
-
 2-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 0.35
-
 3-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 0.36
-
 4-chloromandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 0.47
-
 2-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 0.5
-
 4-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 0.62
-
 3-chloromandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism
3.5.5.1 2.33
-
 mandelonitrile pH 7.5, 40°C, wild-type enzyme uncultured organism
3.5.5.1 17.38
-
 mandelonitrile pH 7.5, 40°C, mutant enzyme S190G uncultured organism