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Literature summary extracted from

  • Benedek, A.; Temesvary-Kis, F.; Khatanbaatar, T.; Leveles, I.; Suranyi, E.V.; Szabo, J.E.; Wunderlich, L.; Vertessy, B.G.
    The role of a key amino acid position in species-specific proteinaceous dUTPase inhibition (2019), Biomolecules, 9, 221 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli (DE3) Rosetta cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 Q93H mutant in complex with a non-hydrolysable substrate analogue, alpha,beta-imido-dUTP, hanging drop vapor diffusion method, using 0.1 M Tris, 18-33.75 % polyethylene glycol 3350, 400 mM NaAc, pH 7.5 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 Q93H the mutant enzyme is significantly inhibited by staphylococcal repressor StlSaPIbov1 protein (Ki 0.00000483 mM) Escherichia coli
3.6.1.23 Q93R the mutant enzyme is significantly inhibited by staphylococcal repressor StlSaPIbov1 protein (Ki 0.0000059 mM) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 additional information the wild type enzyme is not inhibited by staphylococcal repressor StlSaPIbov1 protein Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Escherichia coli
-
 dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Escherichia coli P06968
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Escherichia coli dUMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 DUT
-
 Escherichia coli
3.6.1.23 dUTPase
-
 Escherichia coli