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Literature summary extracted from

  • Tongsook, C.; Niederhauser, J.; Kronegger, E.; Straganz, G.; Macheroux, P.
    Leucine 208 in human histamine N-methyltransferase emerges as a hotspot for protein stability rationalizing the role of the L208P variant in intellectual disability (2017), Biochim. Biophys. Acta, 1863, 188-199 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.8 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.8 L208D mutant is severely compromised in its stability Homo sapiens
2.1.1.8 L208F 21% of wild-type activtiy, Michaelis-Menten parameters for SAM and histamine similar to wild-type Homo sapiens
2.1.1.8 L208H mutant is severely compromised in its stability Homo sapiens
2.1.1.8 L208K substantial loss of enzymatic activity and binding affinity for histamine Homo sapiens
2.1.1.8 L208N mutant is severely compromised in its stability Homo sapiens
2.1.1.8 L208R substantial loss of enzymatic activity and binding affinity for histamine Homo sapiens
2.1.1.8 L208T mutant is severely compromised in its stability Homo sapiens
2.1.1.8 L208V 48% of wild-type activity, Michaelis-Menten parameters for SAM and histamine similar to wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.8 0.0011
-
 histamine mutant L208V, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.0019
-
 histamine wild-type, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.0034
-
 histamine mutant L208F, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.0041
-
 S-adenosyl-L-methionine wild-type, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.0068
-
 S-adenosyl-L-methionine mutant L208V, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.0124
-
 S-adenosyl-L-methionine mutant L208F, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.015
-
 S-adenosyl-L-methionine mutant L208K, pH 8.0, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.8 Homo sapiens P50135
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.8 recombinant protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.8 S-adenosyl-L-methionine + histamine
-
 Homo sapiens S-adenosyl-L-homocysteine + Ntau-methylhistamine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.8 48.4
-
 melting temperature, mutant L208R Homo sapiens
2.1.1.8 53.7
-
 melting temperature, mutant L208K Homo sapiens
2.1.1.8 57.8
-
 melting temperature, mutant L208V Homo sapiens
2.1.1.8 60.4
-
 melting temperature, wild-type, presence of histamine, and melting temeprature of mutant L208F Homo sapiens
2.1.1.8 61.3
-
 melting temperature, wild-type Homo sapiens
2.1.1.8 61.5
-
 melting temperature, wild-type, presence of S-adenosyl-L-methionine Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.8 0.03
-
 S-adenosyl-L-methionine mutant L208K, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.11
-
 S-adenosyl-L-methionine mutant L208F, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.23
-
 S-adenosyl-L-methionine mutant L208V, pH 8.0, 25°C Homo sapiens
2.1.1.8 0.61
-
 S-adenosyl-L-methionine wild-type, pH 8.0, 25°C Homo sapiens