EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.19 | expression in Escherichia coli | Listeria monocytogenes |
2.7.2.7 | expressed in Escherichia coli BL21(DE3) cells | Listeria monocytogenes |
EC Number | General Stability | Organism |
---|---|---|
2.3.1.19 | loss of catalytic activity due to precipitation of purified Ptb is observed at concentrations ranging from 1-6 mg/ml in 50 mM Tris (pH 7.5) and in 25 mM HEPES (pH 7.5) buffers, which is resolved by storage in high NaCl concentrations (1.3-3 M) | Listeria monocytogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.19 | 0.0315 | - |
hexanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.0315 | - |
hexanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.0438 | - |
2-ethylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.0438 | - |
2-ethylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.0481 | - |
2-methylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.0481 | - |
2-methylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.0532 | - |
isovaleryl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.0532 | - |
isovaleryl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.0579 | - |
isobutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.0579 | - |
isobutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.0799 | - |
butanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.0799 | - |
butanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.1608 | - |
pentanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.1608 | - |
pentanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.1906 | - |
propionyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 0.1906 | - |
propionyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.992 | - |
acetyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 0.9921 | - |
acetyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.7.2.7 | 7.6 | - |
Isobutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 13.1 | - |
pentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 16.5 | - |
2-ethylbutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 27.3 | - |
3-methylpentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 36.2 | - |
2-methylbutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 44.2 | - |
2-methylpentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 50.5 | - |
Butanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 57.1 | - |
Isovalerate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 174.1 | - |
propionate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 1856 | - |
hexanoate | at pH 7.5 and 37°C | Listeria monocytogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.7 | Mn2+ | 4 mM used in assay conditions | Listeria monocytogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.19 | Listeria monocytogenes | - |
- |
- |
2.3.1.19 | Listeria monocytogenes 10403S | - |
- |
- |
2.7.2.7 | Listeria monocytogenes | - |
- |
- |
2.7.2.7 | Listeria monocytogenes 10403S | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.7 | Ni-NTA resin column chromatography | Listeria monocytogenes |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.19 | can be stored for two weeks without detectable loss of activity by dilution of eluted protein to below 0.5 mg/ml in HEPES buffer with 1.5 M NaCl | Listeria monocytogenes |
2.3.1.19 | dilution of eluted protein to below 0.5 mg/ml in HEPES buffer with 1.5 M NaCl, storage for two weeks without detectable loss of activity | Listeria monocytogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.19 | 2-ethylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | 2-ethylbutanoyl phosphate + CoA | - |
? | |
2.3.1.19 | 2-ethylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + 2-ethylbutanoyl phosphate | - |
? | |
2.3.1.19 | 2-methylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | 2-methylbutanoyl phosphate + CoA | - |
? | |
2.3.1.19 | 2-methylbutanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + 2-methylbutanoyl phosphate | - |
? | |
2.3.1.19 | acetyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + acetyl phosphate | - |
? | |
2.3.1.19 | acetyl-CoA + phosphate | - |
Listeria monocytogenes 10403S | CoA + acetyl phosphate | - |
? | |
2.3.1.19 | acetyl-CoA + phosphate | - |
Listeria monocytogenes | acetyl Phosphate + CoA | - |
? | |
2.3.1.19 | acetyl-CoA + phosphate | - |
Listeria monocytogenes 10403S | acetyl Phosphate + CoA | - |
? | |
2.3.1.19 | butanoyl-CoA + phosphate | - |
Listeria monocytogenes | butanoyl phosphate + CoA | - |
? | |
2.3.1.19 | butanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + butanoyl phosphate | - |
? | |
2.3.1.19 | hexanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + hexanoyl phosphate | - |
? | |
2.3.1.19 | hexanoyl-CoA + phosphate | - |
Listeria monocytogenes | hexanoyl phosphate + CoA | - |
? | |
2.3.1.19 | isobutanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + isobutanoyl phosphate | - |
? | |
2.3.1.19 | isobutanoyl-CoA + phosphate | - |
Listeria monocytogenes | isobutanoyl phosphate + CoA | - |
? | |
2.3.1.19 | isovaleryl-CoA + phosphate | - |
Listeria monocytogenes | CoA + isovaleryl phosphate | - |
? | |
2.3.1.19 | isovaleryl-CoA + phosphate | - |
Listeria monocytogenes | isovaleryl phosphate + CoA | - |
? | |
2.3.1.19 | additional information | enzyme exhibits a broad substrate specificity, a strong preference for branched-chain substrates, a lack of activity with acetyl CoA and hexanoyl CoA, and strict chain length preference (C3-C5). Catalysis involves ternary complex formation | Listeria monocytogenes | ? | - |
- |
|
2.3.1.19 | additional information | mechanism is consistent with ternary complex formation rather than a ping-pong mechanism. PtB is active with a large number of substrates and prefers substrates with a carbon chain length of C3-C5 and the presence of an alkyl side chain | Listeria monocytogenes | ? | - |
- |
|
2.3.1.19 | additional information | enzyme exhibits a broad substrate specificity, a strong preference for branched-chain substrates, a lack of activity with acetyl CoA and hexanoyl CoA, and strict chain length preference (C3-C5). Catalysis involves ternary complex formation | Listeria monocytogenes 10403S | ? | - |
- |
|
2.3.1.19 | additional information | mechanism is consistent with ternary complex formation rather than a ping-pong mechanism. PtB is active with a large number of substrates and prefers substrates with a carbon chain length of C3-C5 and the presence of an alkyl side chain | Listeria monocytogenes 10403S | ? | - |
- |
|
2.3.1.19 | pentanoyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + pentanoyl phosphate | - |
? | |
2.3.1.19 | pentanoyl-CoA + phosphate | - |
Listeria monocytogenes | pentanoyl phosphate + CoA | - |
? | |
2.3.1.19 | propionyl-CoA + phosphate | - |
Listeria monocytogenes | CoA + propionyl phosphate | - |
? | |
2.3.1.19 | propionyl-CoA + phosphate | - |
Listeria monocytogenes 10403S | CoA + propionyl phosphate | - |
? | |
2.3.1.19 | propionyl-CoA + phosphate | - |
Listeria monocytogenes | propionyl phosphate + CoA | - |
? | |
2.7.2.7 | ATP + 2-ethylbutyrate | - |
Listeria monocytogenes | ADP + 2-ethylbutyryl phosphate | - |
? | |
2.7.2.7 | ATP + 2-methylbutyrate | - |
Listeria monocytogenes | ADP + 2-methylbutyryl phosphate | - |
? | |
2.7.2.7 | ATP + 2-methylpentanoate | - |
Listeria monocytogenes | ADP + 2-methylpentanoyl phosphate | - |
? | |
2.7.2.7 | ATP + 3-methylpentanoate | - |
Listeria monocytogenes | ADP + 3-methylpentanoyl phosphate | - |
? | |
2.7.2.7 | ATP + acetate | - |
Listeria monocytogenes | ADP + acetyl phosphate | - |
? | |
2.7.2.7 | ATP + butanoate | third best substrate | Listeria monocytogenes | ADP + butanoyl phosphate | - |
? | |
2.7.2.7 | ATP + hexanoate | - |
Listeria monocytogenes | ADP + hexanoyl phosphate | - |
? | |
2.7.2.7 | ATP + isobutyrate | second best substrate | Listeria monocytogenes | ADP + isobutyroyl phosphate | - |
? | |
2.7.2.7 | ATP + isovalerate | - |
Listeria monocytogenes | ADP + isovaleroyl phosphate | - |
? | |
2.7.2.7 | ATP + pentanoate | best substrate | Listeria monocytogenes | ADP + pentanoyl phosphate | - |
? | |
2.7.2.7 | ATP + propionate | - |
Listeria monocytogenes | ADP + propionyl phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.19 | ? | x * 34000, SDS-PAGE | Listeria monocytogenes |
2.7.2.7 | ? | x * 42000, SDS-PAGE | Listeria monocytogenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.19 | PTB | - |
Listeria monocytogenes |
2.7.2.7 | Buk | - |
Listeria monocytogenes |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.19 | 47 | - |
hexanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 47 | - |
hexanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 100.9 | - |
2-ethylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 100.9 | - |
2-ethylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 302.4 | - |
acetyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 302.4 | - |
acetyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 518.8 | - |
propionyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 518.8 | - |
propionyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 673.9 | - |
butanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 673.9 | - |
butanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 676.1 | - |
pentanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 676.1 | - |
pentanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 849.1 | - |
2-methylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 849.1 | - |
2-methylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 1043.4 | - |
isovaleryl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 1043.4 | - |
isovaleryl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 1263.4 | - |
isobutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 1263.4 | - |
isobutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.7.2.7 | 0.11 | - |
2-ethylbutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.27 | - |
2-methylbutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.31 | - |
2-methylpentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.35 | - |
3-methylpentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.4 | - |
Isobutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.42 | - |
Isovalerate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.84 | - |
Butanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 1.2 | - |
pentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 2.59 | - |
propionate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 21.85 | - |
hexanoate | at pH 7.5 and 37°C | Listeria monocytogenes |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.19 | 300 | - |
acetyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 300 | - |
acetyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 1700 | - |
hexanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 1700 | - |
hexanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 2300 | - |
2-ethylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 2720 | - |
propionyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 2720 | - |
propionyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 4200 | - |
pentanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 4200 | - |
pentanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 8430 | - |
butanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 8430 | - |
butanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 17700 | - |
2-methylbutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 17700 | - |
2-methylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 19600 | - |
isovaleryl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 19600 | - |
isovaleryl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 21400 | - |
isobutanoyl-CoA | pH 7.5, 25°C | Listeria monocytogenes | |
2.3.1.19 | 21400 | - |
isobutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.3.1.19 | 23000 | - |
2-ethylbutanoyl-CoA | pH 7.5, 23°C | Listeria monocytogenes | |
2.7.2.7 | 0.007 | - |
Isovalerate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.007 | - |
2-methylpentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.007 | - |
2-ethylbutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.011 | - |
hexanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.013 | - |
3-methylpentanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.015 | - |
propionate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.017 | - |
Butanoate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.052 | - |
Isobutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.07 | - |
2-methylbutyrate | at pH 7.5 and 37°C | Listeria monocytogenes | |
2.7.2.7 | 0.092 | - |
pentanoate | at pH 7.5 and 37°C | Listeria monocytogenes |