Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Loring, H.S.; Thompson, P.R.
    Kinetic mechanism of nicotinamide N-methyltransferase (2018), Biochemistry, 57, 5524-5532 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.1 expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.1 1-methylnicotinamide product inhibition Homo sapiens
2.1.1.1 S-adenosyl-L-homocysteine product inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.1 0.003
-
 nicotinamide pH 8.6, 37°C Homo sapiens
2.1.1.1 0.01
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.01
-
 Quinoline pH 8.6, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.1 Homo sapiens P40261
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.1 recombinant protein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.1 additional information NNMT uses a rapid equilibrium ordered mechanism, where NNMT first binds S-adenosyl-L-methionine, which is followed by nicotinamide. Methyl transfer occurs, and methylated nicotinamide and S-adenosylhomocysteine are released consecutively Homo sapiens ?
-
-
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + quinoline
-
 Homo sapiens S-adenosyl-L-homocysteine + N-methylquinoline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.1 0.008
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
2.1.1.1 0.011
-
 Quinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 0.014
-
 nicotinamide pH 8.6, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.1 0.0012
-
 substrate S-adenosyl-L-methionine, pH 8.6, 37°C Homo sapiens S-adenosyl-L-homocysteine
2.1.1.1 0.002
-
 substrate quinoline, pH 8.6, 37°C Homo sapiens S-adenosyl-L-homocysteine
2.1.1.1 0.023
-
 substrate quinoline, pH 8.6, 37°C Homo sapiens 1-methylnicotinamide

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.1 0.75
-
 S-adenosyl-L-methionine pH 8.6, 37°C Homo sapiens
2.1.1.1 1.1
-
 Quinoline pH 8.6, 37°C Homo sapiens
2.1.1.1 4.7
-
 nicotinamide pH 8.6, 37°C Homo sapiens