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Literature summary extracted from

  • Connor, K.L.; Colabroy, K.L.; Gerratana, B.
    A heme peroxidase with a functional role as an L-tyrosine hydroxylase in the biosynthesis of anthramycin (2011), Biochemistry, 50, 8926-8936 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.2.6 expressed in Escherichia coli BL21(DE3) cells Streptomyces refuineus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.2.6 H2O2 the enzyme becomes inactivated by hydrogen peroxide during catalysis Streptomyces refuineus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.6 0.45
-
 L-tyrosine at pH 8.0 and 37°C Streptomyces refuineus
1.11.2.6 1
-
 H2O2 with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Streptomyces refuineus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.2.6 L-tyrosine + H2O2 Streptomyces refuineus
-
 L-dopa + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.6 Streptomyces refuineus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.6 Ni-Sepharose column chromatography or Q-Sepharose column chromatography and S-200HR gel filtration Streptomyces refuineus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.6 3-(4-hydroxylphenyl)propanoic acid + H2O2
-
 Streptomyces refuineus 3-(3,4-hydroxyphenyl)propanoic acid + H2O
-
 ?
1.11.2.6 4-cresol + H2O2
-
 Streptomyces refuineus 4-methylcatechol + H2O
-
 ?
1.11.2.6 DL-tyrosine + H2O2
-
 Streptomyces refuineus L-DOPA + H2O
-
 ?
1.11.2.6 L-tyrosine + H2O2
-
 Streptomyces refuineus L-dopa + H2O
-
 ?
1.11.2.6 additional information no activity with L-phenylalanine Streptomyces refuineus ?
-
-
1.11.2.6 tyramine + H2O2
-
 Streptomyces refuineus dopamine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.2.6 monomer 1 * 33600, SDS-PAGE Streptomyces refuineus
1.11.2.6 monomer 1 * 37900, calculated from amino acid sequence Streptomyces refuineus

Synonyms

EC Number Synonyms Comment Organism
1.11.2.6 Orf13
-
 Streptomyces refuineus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.6 0.576
-
 L-tyrosine at pH 8.0 and 37°C Streptomyces refuineus
1.11.2.6 1.5
-
 H2O2 with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Streptomyces refuineus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.6 heme b required for catalysis Streptomyces refuineus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.6 0.012
-
 L-tyrosine at pH 8.0 and 37°C Streptomyces refuineus
1.11.2.6 0.015
-
 H2O2 with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Streptomyces refuineus