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Literature summary extracted from

  • Quiroga, I.Y.; Pellon-Maison, M.; Suchanek, A.L.; Coleman, R.A.; Gonzalez-Baro, M.R.
    Glycerol-3-phosphate acyltransferases 3 and 4 direct glycerolipid synthesis and affect functionality in activated macrophages (2019), Biochem. J., 476, 85-99 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 gene GPAT3, quantitative real-time PCR expression analysis Mus musculus
2.3.1.15 gene GPAT4, quantitative real-time PCR expression analysis Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 additional information generation of Gpat4-/- mice in a C57BL6/J background Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 NEM GPAT3 is a NEM-sensitive GPAT; GPAT4 is a NEM-sensitive GPAT Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 endoplasmic reticulum
-
 Mus musculus 5783
-
2.3.1.15 membrane
-
 Mus musculus 16020
-
2.3.1.15 microsome
-
 Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Mus musculus
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Mus musculus C57Bl6/J
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Mus musculus Q8C0N2
-
-
2.3.1.15 Mus musculus Q8K2C8
-
-
2.3.1.15 Mus musculus C57Bl6/J Q8C0N2
-
-
2.3.1.15 Mus musculus C57Bl6/J Q8K2C8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 macrophage bone marrow-derived macrophages Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus C57Bl6/J CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus C57Bl6/J CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase 3
-
 Mus musculus
2.3.1.15 glycerol-3-phosphate acyltransferase 4
-
 Mus musculus
2.3.1.15 GPAT3
-
 Mus musculus
2.3.1.15 GPAT4
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 23
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.4
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.15 malfunction the phagocytic capacity of Gpat3-/- and Gpat4-/- bone marrow-derived macrophages is impaired. Additionally, inhibiting fatty acid beta-oxidation reduces phagocytosis only partially, suggesting that lipid accumulation is not necessary for the energy requirements for phagocytosis Mus musculus
2.3.1.15 malfunction the phagocytic capacity of Gpat3-/- and Gpat4-/- bone marrow-derived macrophages is impaired. Additionally, inhibiting fatty acid beta-oxidation reduces phagocytosis only partially, suggesting that lipid accumulation is not necessary for the energy requirements for phagocytosis. Gpat4-/- bone marrow-derived macrophages express and release more pro-inflammatory cytokines and chemokines after macrophage activation Mus musculus
2.3.1.15 metabolism enzyme GPAT is involved in the glycerolipid synthesis, it is the first and rate-limiting enzyme of the pathway. Glycerolipid synthesis and GPAT3/GPAT4 activity are induced during macrophage activation. Macrophage activation stimulates lipid droplet formation and increases triacylglycerol and phospholipid content, after Kdo2-lipid A (KLA) activation Mus musculus
2.3.1.15 physiological function a role for GPAT4 in suppressing inflammatory responses. GPAT4 is required for enhanced glycerolipid synthesis in activated macrophages Mus musculus
2.3.1.15 physiological function GPAT3 is required for enhanced glycerolipid synthesis in activated macrophages Mus musculus