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Literature summary extracted from

  • Paranagama, N.; Bonnett, S.A.; Alvarez, J.; Luthra, A.; Stec, B.; Gustafson, A.; Iwata-Reuyl, D.; Swairjo, M.A.
    Mechanism and catalytic strategy of the prokaryotic-specific GTP cyclohydrolase-IB (2017), Biochem. J., 474, 1017-1039 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.16 overexpressed as an N-terminally His6 tagged protein in Bl21(DE3) cells Neisseria gonorrhoeae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.16 crystals are grown at 20°C by vapor diffusion in sitting drops. High-resolution crystal structures of the enzyme: one in complex with the reaction intermediate analog and competitive inhibitor 8-oxo-GTP, and one with a TRIS molecule bound in the active site and mimicking another reaction intermediate Neisseria gonorrhoeae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.16 C149A mutant enzyme shows no activity Neisseria gonorrhoeae
3.5.4.16 C149A mutant protein purified under the same conditions as wild-type enzyme lacks any S-nitrosylated thiols. S-nitrosylation of the wild-type enzyme occurs posttranslationally during heterologous expression in Escherichia coli and that it is specific to Cys149 Neisseria gonorrhoeae
3.5.4.16 C149S mutant enzyme exhibits 2.7% of the wild-type activity Neisseria gonorrhoeae
3.5.4.16 E152A mutant enzyme exhibits about 20% of the wild-type activity Neisseria gonorrhoeae
3.5.4.16 E243A mutant enzyme exhibits 0.37% of the wild-type activity Neisseria gonorrhoeae
3.5.4.16 H246A mutant enzyme exhibits 0.74% of the wild-type activity Neisseria gonorrhoeae
3.5.4.16 H246D mutant enzyme exhibits 3.0% of the wild-type activity Neisseria gonorrhoeae
3.5.4.16 H246K mutant enzyme shows no activity Neisseria gonorrhoeae
3.5.4.16 H246N mutant enzyme shows no activity Neisseria gonorrhoeae
3.5.4.16 H246Q mutant enzyme exhibits 2.4% of the wild-type activity Neisseria gonorrhoeae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.16 2'-deoxy-GTP
-
Neisseria gonorrhoeae
3.5.4.16 7-deaza-GTP
-
Neisseria gonorrhoeae
3.5.4.16 8-oxo-GTP competitive inhibitor. The inhibitor interacts with a conserved active site Cys149, and this residue is S-nitrosylated. Ki/Km: 0.022 Neisseria gonorrhoeae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.16 0.00915
-
GTP pH and temperature not specified in the publication Neisseria gonorrhoeae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.16 Zn2+ Zn2+-dependent enzyme Neisseria gonorrhoeae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.16 135000
-
gel filtration Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.16 GTP + H2O Neisseria gonorrhoeae
-
formate + 7,8-dihydroneopterin 3'-triphosphate
-
?
3.5.4.16 GTP + H2O Neisseria gonorrhoeae ATCC 700825
-
formate + 7,8-dihydroneopterin 3'-triphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.16 Neisseria gonorrhoeae Q5F9K6
-
-
3.5.4.16 Neisseria gonorrhoeae ATCC 700825 Q5F9K6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.16
-
Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.16 GTP + H2O
-
Neisseria gonorrhoeae formate + 7,8-dihydroneopterin 3'-triphosphate
-
?
3.5.4.16 GTP + H2O
-
Neisseria gonorrhoeae ATCC 700825 formate + 7,8-dihydroneopterin 3'-triphosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.5.4.16 homotetramer
-
Neisseria gonorrhoeae

Synonyms

EC Number Synonyms Comment Organism
3.5.4.16 FelE
-
Neisseria gonorrhoeae
3.5.4.16 GCYH-IB
-
Neisseria gonorrhoeae
3.5.4.16 GTP cyclohydrolase IB
-
Neisseria gonorrhoeae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.16 0.000265
-
GTP pH and temperature not specified in the publication Neisseria gonorrhoeae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.16 0.000149
-
8-oxo-GTP pH and temperature not specified in the publication Neisseria gonorrhoeae
3.5.4.16 0.0457
-
7-deaza-GTP pH and temperature not specified in the publication Neisseria gonorrhoeae
3.5.4.16 9.164
-
2'-deoxy-GTP pH and temperature not specified in the publication Neisseria gonorrhoeae