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Literature summary extracted from

  • Eto, M.; Shindou, H.; Yamamoto, S.; Tamura-Nakano, M.; Shimizu, T.
    Lysophosphatidylethanolamine acyltransferase 2 (LPEAT2) incorporates DHA into phospholipids and has possible functions for fatty acid-induced cell death (2020), Biochem. Biophys. Res. Commun., 526, 246-252 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.23 gene Agpat7, quantitative RT-PCR expression analysis, recombinant overexpression of FLAG-tagged mLPEAT2 in CHO cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.23 H129A site-directed mutagenesis, mutation of a residue in AGPAT motif I (HxxxxD), the mutant shows no LPEAT activity Mus musculus
2.3.1.23 additional information enzyme knockout in Neuro 2A cells via expression of mLPEAT2-siRNA Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.23 additional information
-
 additional information enzyme kinetics Mus musculus
2.3.1.23 0.00314
-
 docosahexanoyl-CoA with 1-octadecenyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication Mus musculus
2.3.1.23 0.0132
-
 docosahexanoyl-CoA with 1-palmitoyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 docosahexaenoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine Mus musculus
-
 CoA + 1-acyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Mus musculus Q6NVG1 enzyme complex
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 adipose tissue low content Mus musculus
-
2.3.1.23 brain predominantly, high expression level of LPEAT2, expression pattern analysis, overview. mLPEAT2 is expressed in all regions, with a comparatively high expression in the cortex Mus musculus
-
2.3.1.23 brain stem
-
 Mus musculus
-
2.3.1.23 cerebellum
-
 Mus musculus
-
2.3.1.23 cerebral cortex anterior and posterior Mus musculus
-
2.3.1.23 colon low content Mus musculus
-
2.3.1.23 corpus striatum
-
 Mus musculus
-
2.3.1.23 heart low content Mus musculus
-
2.3.1.23 hippocampus
-
 Mus musculus
-
2.3.1.23 hypothalamus
-
 Mus musculus
-
2.3.1.23 intestine low content Mus musculus
-
2.3.1.23 kidney low content Mus musculus
-
2.3.1.23 liver low content Mus musculus
-
2.3.1.23 lung
-
 Mus musculus
-
2.3.1.23 muscle low content Mus musculus
-
2.3.1.23 Neuro-2a cell
-
 Mus musculus
-
2.3.1.23 olfactory organ
-
 Mus musculus
-
2.3.1.23 spleen low content Mus musculus
-
2.3.1.23 stomach higher content Mus musculus
-
2.3.1.23 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 docosahexaenoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
-
 Mus musculus CoA + 1-acyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 docosahexaenoyl-CoA + 1-arachidonoyl-sn-glycero-3-phosphoethanolamine
-
 Mus musculus CoA + 1-arachidonoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 docosahexaenoyl-CoA + 1-oleoyl-sn-glycero-3-phosphoethanolamine
-
 Mus musculus CoA + 1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 docosahexaenoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphoethanolamine
-
 Mus musculus CoA + 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 docosahexanoyl-CoA + 1-octadecenyl-sn-glycero-3-phosphoethanolamine reaction of EC 2.3.1.25 Mus musculus CoA + 1-octadecenyl-2-docosahexanoyl-sn-glycero-3-phosphoethanolamine
-
 ?
2.3.1.23 additional information mouse LPEAT2 (mLPEAT2) has LPEAT, LPCAT, and LPGAT activities using 22:6-CoA as a donor. mLPEAT2 shows significant activities for both acyl and alkenyl species Mus musculus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 AGPAT7
-
 Mus musculus
2.3.1.23 LPEAT2
-
 Mus musculus
2.3.1.23 lysophosphatidylethanolamine acyltransferase 2
-
 Mus musculus
2.3.1.23 mLPEAT2
-
 Mus musculus
2.3.1.23 More cf. EC 2.3.1.25 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.23 0.00314
-
 docosahexanoyl-CoA with 1-octadecenyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication Mus musculus
2.3.1.23 0.0132
-
 docosahexanoyl-CoA with 1-palmitoyl-sn-glycero-3-phosphoethanolamine, pH and temperature not specified in the publication Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.23 malfunction Neuro 2A cells overexpressing LPEAT2 underwent cell death with necrotic morphology when differentiated into neuron-like cells, with supplementation with 22:6 (DHA) Mus musculus
2.3.1.23 physiological function lysophosphatidylethanolamine acyltransferase 2 (LPEAT2), one of the enzymes that play a role in the remodeling pathway, has been reported to have lysophosphatidylcholine acyltransferase (LPCAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities with 16:0-CoA, 18:0-CoA, and 18:1-CoA as donors. LPEAT2 incorporates docosahexanoic acid (DHA) into phospholipids and has possible functions for fatty acid-induced cell death. LPEAT2 shows endogenous LPEAT activity with 22:6-CoA, and functions in modulating 22:6/20:4 ratios of phospholipids. LPEAT2 plays a role in inducing cell death DHA-dependently. Cell death induced by DHA is dependent on mLPEAT2, insights on mechanisms of neuronal necrosis Mus musculus