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Literature summary extracted from

  • Yang, S.; Wu, T.; Ding, C.; Zhou, P.; Chen, Z.; Gou, J.
    SAHH and SAMS form a methyl donor complex with CCoAOMT7 for methylation of phenolic compounds (2019), Biochem. Biophys. Res. Commun., 520, 122-127 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 gene At4g26220, recombinant expression of the enzyme in yeast cells, coexpression with enzymes S-adenosyl-L-methionine synthase (SAMS)1, SAMS2, SAMS3, SAMS4, methionine synthase (MS)1, MS2, S-adenosyl-L-homocysteine hydrolase (SAHH)1, and SAHH2 for the yeast two-hybrid interaction analysis, overview. Quantitative real-time reverse transcript-PCR expression analysis Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.104 cell wall
-
 Arabidopsis thaliana 5618
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.104 additional information Arabidopsis thaliana enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo ?
-
-
2.1.1.104 additional information Arabidopsis thaliana Col-0 enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo ?
-
-
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Arabidopsis thaliana Col-0
-
 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + luteolin Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + scoparol
-
 ?
2.1.1.104 S-adenosyl-L-methionine + luteolin Arabidopsis thaliana Col-0
-
 S-adenosyl-L-homocysteine + scoparol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Arabidopsis thaliana Q9C5D7
-
-
2.1.1.104 Arabidopsis thaliana Col-0 Q9C5D7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.104 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 additional information enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo Arabidopsis thaliana ?
-
-
2.1.1.104 additional information CCoAOMT7 in Arabidopsis thaliana shows a strong preference to methylate para-position of flavanones and dihydroflavonols, and also a relatively lower activity on caffeoyl-CoA using S-adenosyl-L-methionine (SAM or AdoMet) as methyl donor to produce feruloyl-CoA. CCoAOMT7 also shows methyltransferase activity on caffeoyl CoA, although it shows the highest activity on luteolin, a kind of flavonoids Arabidopsis thaliana ?
-
-
2.1.1.104 additional information enzyme CCoAOMT7 binds to S-adenosyl-L-homocysteine hydrolase (SAHH) and S-adenosyl-L-methionine synthases (SAMSs) in vivo Arabidopsis thaliana Col-0 ?
-
-
2.1.1.104 additional information CCoAOMT7 in Arabidopsis thaliana shows a strong preference to methylate para-position of flavanones and dihydroflavonols, and also a relatively lower activity on caffeoyl-CoA using S-adenosyl-L-methionine (SAM or AdoMet) as methyl donor to produce feruloyl-CoA. CCoAOMT7 also shows methyltransferase activity on caffeoyl CoA, although it shows the highest activity on luteolin, a kind of flavonoids Arabidopsis thaliana Col-0 ?
-
-
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Arabidopsis thaliana Col-0 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + luteolin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + scoparol
-
 ?
2.1.1.104 S-adenosyl-L-methionine + luteolin
-
 Arabidopsis thaliana Col-0 S-adenosyl-L-homocysteine + scoparol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 caffeoyl CoA methyltransferase
-
 Arabidopsis thaliana
2.1.1.104 caffeoyl CoA methyltransferase 7
-
 Arabidopsis thaliana
2.1.1.104 CCoAOMT
-
 Arabidopsis thaliana
2.1.1.104 CCoAOMT7
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.104 S-adenosyl-L-methionine
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.1.1.104 malfunction interruptions of endogenous SAHH1 by artificial miRNA or SAMSs by T-DNA insertion significantly reduce ferulate contents in the stem cell wall Arabidopsis thaliana
2.1.1.104 physiological function ferulate is methylated from caffeoyl-CoA using S-adenosyl-L-methionine (SAM) as methyl donor catalyzed by caffeoyl CoA methyltransferase (CCoAOMT). Arabidopsis thaliana CCoAOMT7 contributes to ferulate content in the stem cell wall. CCoAOMT7 further binds to S-adenosyl-L-homocysteine hydrolase (SAHH), a critical step in SAM synthesis to release feedback suppression on CCoAOMT. CCoAOMT7 also binds S-adenosyl-L-methionine synthases (SAMSs) in vivo, which is mediated by SAHH1. Enzyme CCoAOMT7 is required for cell wall-bound ferulic esters Arabidopsis thaliana