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Literature summary extracted from

  • Lee, W.; Park, J.; Balasubramanian, P.; Kim, Y.
    Elucidation of the crystal structure of FabD from the multidrug-resistant bacterium Acinetobacter baumannii (2018), Biochem. Biophys. Res. Commun., 505, 208-214 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expressed in Escherichia coli BL21(DE3) cells Acinetobacter baumannii
2.3.1.39 subcloned into NdeI and XhoI sites of the pET28a expression vector, and transformed into Escherichia coli BL21 (DE3) Acinetobacter baumannii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.39 sitting drop vapor diffusion method Acinetobacter baumannii
2.3.1.39 sitting drop vapor diffusion method, using 1.4 M ammonium tartrate dibasic and 0.1 M Tris (pH 8.5) Acinetobacter baumannii

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.39 K195A/K200A the mutation results in a dramatic decrease in acyl-carrier protein binding Acinetobacter baumannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 Trifluoperazine
-
 Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] Acinetobacter baumannii
-
 CoA + a malonyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Acinetobacter baumannii V5VFX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39
-
 Acinetobacter baumannii
2.3.1.39 HiTrap chelating Sepharose column chromatography Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein]
-
 Acinetobacter baumannii CoA + a malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 FabD
-
 Acinetobacter baumannii
2.3.1.39 malonyl CoA-ACP transacylase
-
 Acinetobacter baumannii