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Literature summary extracted from

  • Toyotake, Y.; Cho, H.N.; Kawamoto, J.; Kurihara, T.
    A novel 1-acyl-sn-glycerol-3-phosphate O-acyltransferase homolog for the synthesis of membrane phospholipids with a branched-chain fatty acyl group in Shewanella livingstonensis Ac10 (2018), Biochem. Biophys. Res. Commun., 500, 704-709 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 gene plsC4, DNA and amino acid sequence determination and analysis, recombinant LI3-regulated expression of C-terminally His6-tagged PlsC4 in Escherichia coli strain S17-1/lambdapir Shewanella livingstonensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.51 additional information generation of a plsC4-disrupted mutant, phenotype, overview Shewanella livingstonensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.51 membrane
-
 Shewanella livingstonensis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Shewanella livingstonensis
-
 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Shewanella livingstonensis Ac10
-
 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Shewanella livingstonensis A0A3G9EMC4
-
-
2.3.1.51 Shewanella livingstonensis Ac10 A0A3G9EMC4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Shewanella livingstonensis CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Shewanella livingstonensis Ac10 CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.51 1-acyl-sn-glycerol-3-phosphate O-acyltransferase
-
 Shewanella livingstonensis
2.3.1.51 PlsC
-
 Shewanella livingstonensis
2.3.1.51 PlsC4
-
 Shewanella livingstonensis

General Information

EC Number General Information Comment Organism
2.3.1.51 malfunction in a plsC4-disrupted mutant, phospholipids (PLs) containing 13:0 found in the parental strain are almost completely absent. The loss of these PLs is suppressed by introduction of a plsC4-expression plasmid. PLs containing 15:0 are also drastically decreased by plsC4 disruption Shewanella livingstonensis
2.3.1.51 physiological function the 1-acyl-sn-glycerol-3-phosphate O-acyltransferase homologue is responsible for the synthesis of membrane phospholipids with a branched-chain fatty acyl group in Shewanella livingstonensis Ac10. 1-Acyl-sn-glycerol-3-phosphate O-acyltransferase (PlsC) plays an essential role in the formation of phosphatidic acid, a precursor of various membrane phospholipids (PLs), in bacteria by catalyzing the introduction of an acyl group into the sn-2 position of lysophosphatidic acid. Isozyme PlsC4 is a distinct type of PlsC homologue that is responsible for the synthesis of PLs containing a branched-chain fatty acyl group at the sn-2 position and plays a clearly different role from that of PlsC1 in vivo. Gas chromatography-mass spectrometry analysis of fatty acyl methyl esters derived from PLs of the PlsC4 parental strain shows that the 13:0 and 15:0 groups are an 11-methyllauroyl group and a 13-methylmyristoyl group, respectively. Phospholipase A2 treatment reveals that these fatty acyl groups are linked to the sn-2 position of PLs Shewanella livingstonensis