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Literature summary extracted from

  • Moncada, R.M.; Blackshear, K.J.; Garrett, T.A.
    The Arabidopsis thaliana lysophospholipid acyltransferase At1g78690p acylates lysocardiolipins (2017), Biochem. Biophys. Res. Commun., 493, 340-345 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.23 gene At1g78690, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3)pLysS Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylethanolamine Arabidopsis thaliana
-
 CoA + 1,2-diacyl-lysophosphatidylethanolamine
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylglycerol Arabidopsis thaliana
-
 CoA + 1,2-diacyl-lysophosphatidylglycerol
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylinositol Arabidopsis thaliana
-
 CoA + 1,2-diacyl-lysophosphatidylinositol
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylserine Arabidopsis thaliana
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 CoA + 1,2-diacyl-lysophosphatidylserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Arabidopsis thaliana Q9ZV87
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylethanolamine
-
 Arabidopsis thaliana CoA + 1,2-diacyl-lysophosphatidylethanolamine
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylglycerol
-
 Arabidopsis thaliana CoA + 1,2-diacyl-lysophosphatidylglycerol
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylinositol
-
 Arabidopsis thaliana CoA + 1,2-diacyl-lysophosphatidylinositol
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylserine
-
 Arabidopsis thaliana CoA + 1,2-diacyl-lysophosphatidylserine
-
 ?
2.3.1.23 additional information Arabidopsis thaliana lysophospholipid acyltransferase utilizes both mono- and dilysocardiolipin as an acyl acceptor. The enzyme robustly acylates 1-acyl-lysophosphatidylethanolamine (PE), 1-acyl-lysophosphatidylglycerol (PG), 1-acyl lysophosphatidylserine (PS), and 1-acyl-phosphatidylinositol (PI) using acyl-CoA as the acyl donor to produce PE, PG, PS, and PI, respectively. Di-linoleoylphosphatidylcholine is used as the acyl donor and mono-lysocardiolipin is used as the acyl acceptor in a reaction. No transfer of the linoleoyl chains is detected in an At1g78690-dependent manner suggesting that, despite the strong homology, the cardiolipin remodeling enzyme tafazzin and the lysophospholipid acyltransferase catalyze unique reactions. No 18:2 acyl chains are detected in other phospholipid pools in an At1g78690-dependent manner. Enzyme At1g78690 does not function as a transacylase using phosphatidyylcholine as an acyl donor. Mass spectrometric identification of in vitro products Arabidopsis thaliana ?
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-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 At1g78690
-
 Arabidopsis thaliana
2.3.1.23 lysophospholipid acyltransferase
-
 Arabidopsis thaliana
2.3.1.23 N-acylphosphatidylethanolamine synthase UniProt Arabidopsis thaliana
2.3.1.23 NAPE synthase UniProt Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.23 7.4
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.3.1.23 evolution enzyme At1g78690 shares high homology (about 40%) with the cardiolipin remodeling enzyme tafazzin, but the cardiolipin remodeling enzyme tafazzin and the lysophospholipid acyltransferase catalyze unique reactions Arabidopsis thaliana
2.3.1.23 malfunction despite di-acylate phosphatidylglycerol being a substrate, overexpression of At1g78690 in Escherichia coli leads to the accumulation of acyl-PG. Cardiolipin also accumulates in cells overexpressing At1g78690, cardiolipin is found in the inner mitochondrial membrane in eukaryotes and is critical for mitochondrial function. It serves as a necessary proton trap for oxidative phosphorylation and is a trigger for apoptosis Arabidopsis thaliana