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Literature summary extracted from

  • Tumhom, S.; Krusong, K.; Pongsawasdi, P.
    Y418 in 410s loop is required for high transglucosylation activity and large-ring cyclodextrin production of amylomaltase from Corynebacterium glutamicum (2017), Biochem. Biophys. Res. Commun., 488, 516-521 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.25 Y418A the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
2.4.1.25 Y418D the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
2.4.1.25 Y418F the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 29-33 from pea starch Corynebacterium glutamicum
2.4.1.25 Y418R the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
2.4.1.25 Y418S the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum
2.4.1.25 Y418W the mutant shows a significant decrease in starch transglucosylation, disproportionation and cyclization activities compared to the wild type enzyme. The mutant produces large ring-cyclodextrins 36-40 from pea starch Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 15.7
-
 maltotriose mutant enzyme Y418A, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 16.3
-
 maltotriose mutant enzyme Y418D, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 17.2
-
 maltotriose wild type enzyme, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 18
-
 maltotriose mutant enzyme Y418S, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 18.2
-
 maltotriose mutant enzyme Y418F, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 19.5
-
 maltotriose mutant enzyme Y418R, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 20.4
-
 maltotriose mutant enzyme Y418W, at pH 6.0 and 30°C Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Corynebacterium glutamicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltoheptaose + glycosyl acceptor worst substrate Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltohexaose + glycosyl acceptor
-
 Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + glycosyl acceptor
-
 Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotetraose + glycosyl acceptor second best substrate Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotriose + glycosyl acceptor best substrate Corynebacterium glutamicum maltose + D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 pea starch + glycosyl acceptor
-
 Corynebacterium glutamicum large ring-cyclodextrins 29-33
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 ? x * 84000, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 amylomaltase
-
 Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 258
-
 maltotriose mutant enzyme Y418R, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 403
-
 maltotriose mutant enzyme Y418W, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 1833
-
 maltotriose mutant enzyme Y418S, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 1867
-
 maltotriose mutant enzyme Y418D, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 1967
-
 maltotriose mutant enzyme Y418A, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 2667
-
 maltotriose mutant enzyme Y418F, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 2800
-
 maltotriose wild type enzyme, at pH 6.0 and 30°C Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.25 13.3
-
 maltotriose mutant enzyme Y418R, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 19.7
-
 maltotriose mutant enzyme Y418W, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 101.8
-
 maltotriose mutant enzyme Y418S, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 115
-
 maltotriose mutant enzyme Y418D, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 125.3
-
 maltotriose mutant enzyme Y418A, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 146.7
-
 maltotriose mutant enzyme Y418F, at pH 6.0 and 30°C Corynebacterium glutamicum
2.4.1.25 163.3
-
 maltotriose wild type enzyme, at pH 6.0 and 30°C Corynebacterium glutamicum