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Literature summary extracted from

  • Sun, H.; Wang, H.; Gao, W.; Chen, L.; Wu, K.; Wei, D.
    Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile (2015), Biochem. Biophys. Res. Commun., 468, 820-825 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.5 expression in Escherichia coli BL21 (DE3) Pseudomonas psychrotolerans

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.5 A136Y mutant enzyme shows reversed selectivity and preferentially produces (R)-mandelic acid with an enantiomeric excess values of 66.7% Pseudomonas psychrotolerans
3.5.5.5 A136Y/I168Y mutant enzyme produces (R)-mandelic acid with an enantiomeric excess value of 89.7%, an R-enantioselectivity higher than that obtained with the single mutations A136Y and I168Y Pseudomonas psychrotolerans
3.5.5.5 A136Y/I168Y/M113G mutant enzyme with R-selectivity, 90.9% enantiomeric excess Pseudomonas psychrotolerans
3.5.5.5 I168Y mutant enzyme shows reversed selectivity and preferentially produces (R)-mandelic acid with an enantiomeric excess values of 74.3% Pseudomonas psychrotolerans
3.5.5.5 M113C mutation enhances the S-enantioselectivity of the enzyme toward mandelonitrile Pseudomonas psychrotolerans
3.5.5.5 M113G/A136Y/I168Y R-selectivity toward mandelonitrile with 90.1% enantiomeric excess Pseudomonas psychrotolerans
3.5.5.5 M113L/R128H S-selectivity toward mandelonitrile with 91.1% enantiomeric excess Pseudomonas psychrotolerans
3.5.5.5 M113Q mutation enhances the S-enantioselectivity of the enzyme toward mandelonitrile Pseudomonas psychrotolerans
3.5.5.5 R128E mutant displays no activity toward mandelonitrile Pseudomonas psychrotolerans
3.5.5.5 R128H mutation enhances the S-enantioselectivity of the enzyme toward mandelonitrile Pseudomonas psychrotolerans
3.5.5.5 R128K mutant demonstrates low enantioselectivity (44.9% enantiomeric excess) as compared with the wild-type enzyme (52.7% enantiomeric excess) Pseudomonas psychrotolerans

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Pseudomonas psychrotolerans
-
-
-
3.5.5.5 Pseudomonas psychrotolerans L19
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 (R,S)-mandelonitrile + 2 H2O the enzyme hydrolyzes racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess value of 52.7% Pseudomonas psychrotolerans (S)-mandelic acid + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 2 H2O the enzyme hydrolyzes racemic mandelonitrile to (S)-mandelic acid with an enantiomeric excess value of 52.7% Pseudomonas psychrotolerans L19 (S)-mandelic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.5 nitrilase PpL19
-
 Pseudomonas psychrotolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.5 30
-
 assay at Pseudomonas psychrotolerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.5 7
-
 assay at Pseudomonas psychrotolerans