Literature summary extracted from
Lisheng, X.; Guizhen, G.; Xingtao, Z.; Mengting, W.
Enzymatic synthesis of gamma-glutamylmethylamide from L-glutamylhydrazine and methylamine catalysed by immobilized recombinant gamma-glutamyltranspeptidase (2019), Biocatal. Biotransform., 37, 86-91 .
No PubMed abstract available
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.3.2.2 |
additional information |
immobilization of recombinant enzyme on 3% sodium alginates inpresence of 2% CaCl2, immobilized recombinant gamma-glutamyltranspeptidase cells exhibit favourable operational stability for enzymatic synthesis of gamma-glutamylmethylamide from L-glutamylhydrazine and methylamine, usage of L-glutamylhydrazine as an economical substrate, immobilized gamma-glutamyltranspeptidase cells are used for 10 reaction cycles, and the average conversion ratio from L-glutamylhydrazine to gamma-glutamylmethylamide reaches 93.2%. No inhibition by 200 mM L-glutamylhydrazine |
Escherichia coli |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.2.2 |
additional information |
no inhibition of recombinant enzyme by 200 mM L-glutamylhydrazine |
Escherichia coli |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.2.2 |
Escherichia coli |
P18956 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.2.2 |
L-glutamylhydrazine + methylamine |
recombinant enzyme, method optimmization and evaluation, overview |
Escherichia coli |
gamma-L-glutamylmethylamide + hydrazine |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.2.2 |
gamma-glutamyltranspeptidase |
- |
Escherichia coli |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.3.2.2 |
37 |
- |
- |
Escherichia coli |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
2.3.2.2 |
20 |
50 |
over 40% activity within this range, profile overview |
Escherichia coli |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.3.2.2 |
10 |
- |
- |
Escherichia coli |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.3.2.2 |
5.5 |
12 |
activity range, over 60% of maximal activity at pH 7.5-10.5, profile overview |
Escherichia coli |