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Literature summary extracted from

  • Lisheng, X.; Guizhen, G.; Xingtao, Z.; Mengting, W.
    Enzymatic synthesis of gamma-glutamylmethylamide from L-glutamylhydrazine and methylamine catalysed by immobilized recombinant gamma-glutamyltranspeptidase (2019), Biocatal. Biotransform., 37, 86-91 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.2 additional information immobilization of recombinant enzyme on 3% sodium alginates inpresence of 2% CaCl2, immobilized recombinant gamma-glutamyltranspeptidase cells exhibit favourable operational stability for enzymatic synthesis of gamma-glutamylmethylamide from L-glutamylhydrazine and methylamine, usage of L-glutamylhydrazine as an economical substrate, immobilized gamma-glutamyltranspeptidase cells are used for 10 reaction cycles, and the average conversion ratio from L-glutamylhydrazine to gamma-glutamylmethylamide reaches 93.2%. No inhibition by 200 mM L-glutamylhydrazine Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 additional information no inhibition of recombinant enzyme by 200 mM L-glutamylhydrazine Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Escherichia coli P18956
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 L-glutamylhydrazine + methylamine recombinant enzyme, method optimmization and evaluation, overview Escherichia coli gamma-L-glutamylmethylamide + hydrazine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 gamma-glutamyltranspeptidase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.2 20 50 over 40% activity within this range, profile overview Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 10
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 5.5 12 activity range, over 60% of maximal activity at pH 7.5-10.5, profile overview Escherichia coli