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Literature summary extracted from

  • Chi, M.; Lo, H.; Lin, M.; Chen, Y.; Lin, L.; Wang, T.
    Application of Bacillus licheniformis gamma-glutamyltranspeptidase to the biocatalytic synthesis of gamma-glutamyl-phenylalanine (2017), Biocatal. Agricult. Biotechnol., 10, 278-284 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.3.2.2 synthesis using recombinant Bacillus licheniformis gamma-glutamyltranspeptidase (BlGGT) in a straightforward procedure for the biocatalytic synthesis of gamma-glutamyl-phenylalanine (gamma-Glu-Phe), qualitative analysis of reaction products, mass spectrometry/nuclear magnetic resonance analyses Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 gene ggt, recombinant expression of His-tagged enzyme GGT in Escherichia coli strain M15 Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid Bacillus licheniformis
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 a peptide + a 5-L-glutamyl amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus licheniformis A0A415J2H1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 recombinant His-tagged GGT from Escherichia coli strain M15 by nickel affinity chromatography Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus licheniformis a peptide + a 5-L-glutamyl amino acid
-
 ?
2.3.2.2 L-gamma-glutamyl-4-nitroanilide + L-phenylalanine the synthetic substrate L-gamma-Glu-4-nitroanilide is used as a gamma-glutamyl donor, biocatalytic synthesis of gamma-Glu-Phe, reaction via formation of a gamma-glutamyl-enzyme intermediate. The intermediate can then react with water to release glutamate in a hydrolysis reaction or with amino acids or peptides to produce other gamma-glutamyl compounds in a transpeptidation reaction. Product analysis by thin layer chromatography Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 BlGGT
-
 Bacillus licheniformis
2.3.2.2 gamma-glutamyltranspeptidase
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 50
-
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.2 59.5 70 the melting temperature of enzyme BlGGT is around 59.5°C, the irreversible denaturation of this enzyme occurs at 70°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 9
-
 optimal pH for gamma-Glu-Phe synthesis Bacillus licheniformis