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Literature summary extracted from

  • Mazmouz, R.; Essadik, I.; Hamdane, D.; Mejean, A.; Ploux, O.
    Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis Binding studies, site-directed mutagenesis and stereoselectivity (2018), Arch. Biochem. Biophys., 647, 1-9 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.70 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Oscillatoria sp. PCC 7926

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.70 D159A inactive Oscillatoria sp. PCC 7926
1.14.11.70 H157A inactive Oscillatoria sp. PCC 7926
1.14.11.70 H247A inactive Oscillatoria sp. PCC 7926
1.14.11.70 R257A inactive Oscillatoria sp. PCC 7926

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.70 7-deoxycylindrospermopsin the enzyme is strongly inhibited by 7-deoxycylindrospermopsin at concentration higher than 0.001 mM Oscillatoria sp. PCC 7926

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.70 0.0032
-
 2-oxoglutarate pH 7.4 and 28°C Oscillatoria sp. PCC 7926
1.14.11.70 0.019
-
 7-deoxycylindrospermopsin pH 7.4 and 28°C Oscillatoria sp. PCC 7926

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.70 Fe2+ dependent on. Km value is 0.0021 mM. 0.05 mM used in assay conditions Oscillatoria sp. PCC 7926

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 cylindrospermopsin + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.70 Oscillatoria sp. PCC 7926
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.70 His-Trap nickel column chromatography and Superdex S200 gel filtration Oscillatoria sp. PCC 7926

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
1.14.11.70 7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 cylindrospermopsin + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.70 cyrI
-
 Oscillatoria sp. PCC 7926

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.70 0.03
-
 2-oxoglutarate pH 7.4 and 28°C Oscillatoria sp. PCC 7926

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.70 38.3
-
 7-deoxycylindrospermopsin pH 7.4 and 28°C Oscillatoria sp. PCC 7926