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Literature summary extracted from

  • Jia, Y.; Wang, J.; Ren, C.; Nahurira, R.; Khokhar, I.; Wang, J.; Fan, S.; Yan, Y.
    Identification and characterization of a meta-cleavage product hydrolase involved in biphenyl degradation from Arthrobacter sp. YC-RL1 (2019), Appl. Microbiol. Biotechnol., 103, 6825-6836 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.7.1.8 environmental protection potential enzyme resource for the biodegradation of biphenyl, bioremediation of the environmental pollution caused by biphenyl/polychlorinated biphenyls Arthrobacter sp. YC-RL1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.8 heterologously expressed in Escherichia coli BL21 (DE3) Arthrobacter sp. YC-RL1

Protein Variants

EC Number Protein Variants Comment Organism
3.7.1.8 D247A mutant enzyme retains 29.93% enzyme activity Arthrobacter sp. YC-RL1
3.7.1.8 H275A mutant enzyme retains 10.25% enzyme activity Arthrobacter sp. YC-RL1
3.7.1.8 S115A mutant enzyme retains 28.92% enzyme activity Arthrobacter sp. YC-RL1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.8 CHAPS
-
Arthrobacter sp. YC-RL1
3.7.1.8 DEPC
-
Arthrobacter sp. YC-RL1
3.7.1.8 DTT
-
Arthrobacter sp. YC-RL1
3.7.1.8 additional information insensitive to EDTA Arthrobacter sp. YC-RL1
3.7.1.8 NBS
-
Arthrobacter sp. YC-RL1
3.7.1.8 PMSF
-
Arthrobacter sp. YC-RL1
3.7.1.8 SDS
-
Arthrobacter sp. YC-RL1
3.7.1.8 Trition X-100
-
Arthrobacter sp. YC-RL1
3.7.1.8 Tween 20
-
Arthrobacter sp. YC-RL1
3.7.1.8 Tween 80
-
Arthrobacter sp. YC-RL1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.8 0.14
-
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate pH 7.0, 30°C Arthrobacter sp. YC-RL1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.8 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O Arthrobacter sp. YC-RL1 the enzyme is involved in the biodegradation of biphenyl 2-oxopent-4-enoate + salicylate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Arthrobacter sp. YC-RL1 A0A0H0ZQK0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.8
-
Arthrobacter sp. YC-RL1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O
-
Arthrobacter sp. YC-RL1 2-oxopent-4-enoate + salicylate
-
?
3.7.1.8 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O the enzyme is involved in the biodegradation of biphenyl Arthrobacter sp. YC-RL1 2-oxopent-4-enoate + salicylate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.8 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase
-
Arthrobacter sp. YC-RL1
3.7.1.8 BphD
-
Arthrobacter sp. YC-RL1
3.7.1.8 HOPDA hydrolase
-
Arthrobacter sp. YC-RL1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.8 45 50
-
Arthrobacter sp. YC-RL1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.7.1.8 15 55 15°C: about 45% of maximal activity, 55°C: about 70% of maximal activity Arthrobacter sp. YC-RL1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.8 40
-
1 h, the enzyme retains more than 60% residual activity Arthrobacter sp. YC-RL1
3.7.1.8 60
-
1 h, the enzyme retains less than 60% residual activity Arthrobacter sp. YC-RL1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.8 11.61
-
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate pH 7.0, 30°C Arthrobacter sp. YC-RL1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.8 7
-
-
Arthrobacter sp. YC-RL1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.7.1.8 5 10 pH 5.0: about 65% of maximal activity, pH 9.0: about 85% of maximal activity, pH 10.0: about 35 % of maximal activity Arthrobacter sp. YC-RL1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.7.1.8 5 9 more than 60% of the activity is retained after incubation at pH 5.0-9.0 at 30°C for 10 min Arthrobacter sp. YC-RL1

General Information

EC Number General Information Comment Organism
3.7.1.8 metabolism the enzyme is involved in the biodegradation of biphenyl Arthrobacter sp. YC-RL1