EC Number | Application | Comment | Organism |
---|---|---|---|
3.7.1.8 | environmental protection | potential enzyme resource for the biodegradation of biphenyl, bioremediation of the environmental pollution caused by biphenyl/polychlorinated biphenyls | Arthrobacter sp. YC-RL1 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.7.1.8 | heterologously expressed in Escherichia coli BL21 (DE3) | Arthrobacter sp. YC-RL1 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.7.1.8 | D247A | mutant enzyme retains 29.93% enzyme activity | Arthrobacter sp. YC-RL1 |
3.7.1.8 | H275A | mutant enzyme retains 10.25% enzyme activity | Arthrobacter sp. YC-RL1 |
3.7.1.8 | S115A | mutant enzyme retains 28.92% enzyme activity | Arthrobacter sp. YC-RL1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.7.1.8 | CHAPS | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | DEPC | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | DTT | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | additional information | insensitive to EDTA | Arthrobacter sp. YC-RL1 | |
3.7.1.8 | NBS | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | PMSF | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | SDS | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | Trition X-100 | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | Tween 20 | - |
Arthrobacter sp. YC-RL1 | |
3.7.1.8 | Tween 80 | - |
Arthrobacter sp. YC-RL1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.7.1.8 | 0.14 | - |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate | pH 7.0, 30°C | Arthrobacter sp. YC-RL1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.7.1.8 | 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O | Arthrobacter sp. YC-RL1 | the enzyme is involved in the biodegradation of biphenyl | 2-oxopent-4-enoate + salicylate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.7.1.8 | Arthrobacter sp. YC-RL1 | A0A0H0ZQK0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.7.1.8 | - |
Arthrobacter sp. YC-RL1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.7.1.8 | 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O | - |
Arthrobacter sp. YC-RL1 | 2-oxopent-4-enoate + salicylate | - |
? | |
3.7.1.8 | 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate + H2O | the enzyme is involved in the biodegradation of biphenyl | Arthrobacter sp. YC-RL1 | 2-oxopent-4-enoate + salicylate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.7.1.8 | 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoate hydrolase | - |
Arthrobacter sp. YC-RL1 |
3.7.1.8 | BphD | - |
Arthrobacter sp. YC-RL1 |
3.7.1.8 | HOPDA hydrolase | - |
Arthrobacter sp. YC-RL1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.7.1.8 | 45 | 50 | - |
Arthrobacter sp. YC-RL1 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.7.1.8 | 15 | 55 | 15°C: about 45% of maximal activity, 55°C: about 70% of maximal activity | Arthrobacter sp. YC-RL1 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.7.1.8 | 40 | - |
1 h, the enzyme retains more than 60% residual activity | Arthrobacter sp. YC-RL1 |
3.7.1.8 | 60 | - |
1 h, the enzyme retains less than 60% residual activity | Arthrobacter sp. YC-RL1 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.7.1.8 | 11.61 | - |
2-hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate | pH 7.0, 30°C | Arthrobacter sp. YC-RL1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.7.1.8 | 7 | - |
- |
Arthrobacter sp. YC-RL1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.7.1.8 | 5 | 10 | pH 5.0: about 65% of maximal activity, pH 9.0: about 85% of maximal activity, pH 10.0: about 35 % of maximal activity | Arthrobacter sp. YC-RL1 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.7.1.8 | 5 | 9 | more than 60% of the activity is retained after incubation at pH 5.0-9.0 at 30°C for 10 min | Arthrobacter sp. YC-RL1 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.7.1.8 | metabolism | the enzyme is involved in the biodegradation of biphenyl | Arthrobacter sp. YC-RL1 |