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Literature summary extracted from
- Metagenome-derived haloalkane dehalogenases with novel catalytic properties (2017), Appl. Microbiol. Biotechnol., 101, 6385-6397 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | expression in Escherichia coli BL21 Star (DE3) cells | uncultured bacterium |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 37400 | - |
gel filtration, enzyme form eHLD-C | uncultured bacterium |
| 3.8.1.5 | 37900 | - |
gel filtration, enzyme form eHLD-B | uncultured bacterium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | uncultured bacterium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | - |
uncultured bacterium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 4.86 | - |
substrate: 1,3-dibromopropane, enzyme form eHLD-B, pH and temperature not specified in the publication | uncultured bacterium |
| 3.8.1.5 | 12.2 | - |
substrate: 1,2-dibromoethane, enzyme form eHLD-C, pH and temperature not specified in the publication | uncultured bacterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | (1-bromomethyl)cyclohexane + H2O | 2.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | ? | - |
? |  |
| 3.8.1.5 | (1-bromomethyl)cyclohexane + H2O | less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | ? | - |
? | |
| 3.8.1.5 | 1,2,3-tribromopropane + H2O | about 50% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2,3-dibromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromo-3-chloropropane + H2O | about 40% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-bromo-3-chloro-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromo-3-chloropropane + H2O | about 5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 2-bromo-3-chloro-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | about 25% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 2-bromo-1-ethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | the highest specific activity of enzyme form eHLD-C is determined for 1,2-dibromoethane and 1-bromo-2-chloroethane | uncultured bacterium | 2-bromo-1-ethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromopropane + H2O | about 65% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-bromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-chloro-1-ethanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dichloropropane + H2O | less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-chloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,3-dibromopropane + H2O | about 40% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 3-bromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,3-dibromopropane + H2O | the highest specific activity of enzyme form eHLD-B is determined for 1,3-dibromopropane and 1-bromo-3-chloropropane | uncultured bacterium | 3-bromo-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1,3-dichloropropane + H2O | about 10% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 3-chloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,3-diiodopropane + H2O | 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 3-iodo-1-propanol + iodide | - |
? | |
| 3.8.1.5 | 1,3-diiodopropane + H2O | about 35% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 3-iodo-1-propanol + iodide | - |
? | |
| 3.8.1.5 | 1,5-dichloropentane + H2O | 3% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 5-chloro-1-pentanol + chloride | - |
? | |
| 3.8.1.5 | 1,5-dichloropentane + H2O | about 45% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 5-chloro-1-pentanol + chloride | - |
? | |
| 3.8.1.5 | 1-bromo-2-chloroethane + H2O | about 10% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 2-chloro-1-ethanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromo-2-chloroethane + H2O | about 85% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-chloro-1-ethanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromo-3-chloropropane + H2O | about 75% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 3-chloro-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromo-3-chloropropane + H2O | about 80% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 3-chloro-1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromobutane + H2O | about 35% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-butanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromobutane + H2O | about 60% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 1-butanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromohexane + H2O | 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-hexanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromohexane + H2O | about 20% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 1-hexanol + bromide | - |
? | |
| 3.8.1.5 | 1-chlorobutane + H2O | less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-butanol + chloride | - |
? | |
| 3.8.1.5 | 1-chlorohexane + H2O | about 20% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 1-hexanol + chloride | - |
? | |
| 3.8.1.5 | 1-chlorohexane + H2O | less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-hexanol + chloride | - |
? | |
| 3.8.1.5 | 1-iodobutane + H2O | 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-butanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodobutane + H2O | about 25% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 1-butanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodohexane + H2O | 2.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 1-hexanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodohexane + H2O | less than 1% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-hexanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodopropane + H2O | 5% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 1-propanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodopropane + H2O | about 15% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 1-propanol + iodide | - |
? | |
| 3.8.1.5 | 2,3-dichloropropene + H2O | about 15% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | ? | - |
? | |
| 3.8.1.5 | 2,3-dichloropropene + H2O | about 5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | ? | - |
? | |
| 3.8.1.5 | 2-bromo-1-chloropropane + H2O | about 50% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 2-bromo-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 2-bromo-1-chloropropane + H2O | about 60% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-bromo-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 2-iodobutane + H2O | 3% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 2-butanol + iodide | - |
? | |
| 3.8.1.5 | 2-iodobutane + H2O | 7.5% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 2-butanol + iodide | - |
? | |
| 3.8.1.5 | 3-chloro-2-methylpropene + H2O | about 15% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | ? | - |
? | |
| 3.8.1.5 | 3-chloro-2-methylpropene + H2O | about 15% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | ? | - |
? | |
| 3.8.1.5 | 4-bromobutyronitrile + H2O | about 25% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | 4-hydroxybutyronitrile + bromide | - |
? | |
| 3.8.1.5 | 4-bromobutyronitrile + H2O | about 75% of the activity compared to 1,3-dibromopropane, enzyme form eHLD-B | uncultured bacterium | 4-hydroxybutyronitrile + bromide | - |
? | |
| 3.8.1.5 | bis(2-chloroethyl)ether + H2O | 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | ? | - |
? | |
| 3.8.1.5 | bromocyclohexane + H2O | 4% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | cyclohexanol + bromide | - |
? | |
| 3.8.1.5 | chlorocyclopentane + H2O | about 10% of the activity compared to 1,2-dibromoethane, enzyme form eHLD-C | uncultured bacterium | cyclopentanol + chloride | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | eHLD-B | - |
uncultured bacterium |
| 3.8.1.5 | eHLD-C | - |
uncultured bacterium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 25 | - |
at pH 8.5, enzyme form eHLD-B | uncultured bacterium |
| 3.8.1.5 | 55 | - |
at pH 6.5, enzyme form eHLD-C | uncultured bacterium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 34 | 35.1 | melting temperature, enzyme form eHLD-B | uncultured bacterium |
| 3.8.1.5 | 55.5 | 58.7 | melting temperature, enzyme form eHLD-C | uncultured bacterium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 5.5 | 8.9 | broad optimum with a plateau region of maximum activity | uncultured bacterium |
| 3.8.1.5 | 8.5 | - |
enzyme form eHLD-B | uncultured bacterium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 4.7 | 10 | pH 4.7: about % of maximal activity, pH 10.0: about % of maximal activity, enzyme form eHLD-C | uncultured bacterium |
| 3.8.1.5 | 6 | 9.7 | pH 6.0: about % of maximal activity, pH 9.7: about % of maximal activity, enzyme form eHLD-B | uncultured bacterium |
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