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Literature summary extracted from

  • Vesela, A.B.; Rucka, L.; Kaplan, O.; Pelantova, H.; Ne?vera, J.; Patek, M.; Martinkova, L.
    Bringing nitrilase sequences from databases to life the search for novel substrate specificities with a focus on dinitriles (2016), Appl. Microbiol. Biotechnol., 100, 2193-2202 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Aspergillus luchuensis G7XTA8
-
-
3.5.5.1 Aspergillus luchuensis IFO 4308 G7XTA8
-
-
3.5.5.5 Aspergillus luchuensis G7X8S6
-
-
3.5.5.5 Aspergillus luchuensis IFO 4308 G7X8S6
-
-
3.5.5.5 Auricularia delicata A0A0P1DJE3
-
-
3.5.5.5 Auricularia delicata TFB-10046 SS5 A0A0P1DJE3
-
-
3.5.5.5 Macrophomina phaseolina K2QXC4
-
-
3.5.5.5 Macrophomina phaseolina MS6 K2QXC4
-
-
3.5.5.5 Trichoderma virens Gv29-8 G9N4E3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
 Aspergillus luchuensis
3.5.5.5
-
 Auricularia delicata
3.5.5.5
-
 Aspergillus luchuensis
3.5.5.5
-
 Macrophomina phaseolina
3.5.5.5
-
 Trichoderma virens Gv29-8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 1,2-phenylenediacetonitrile + H2O
-
 Aspergillus luchuensis 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.1 1,3-phenylenediacetonitrile + H2O
-
 Aspergillus luchuensis 1,3-phenylenediacetic acid + NH3
-
 ?
3.5.5.1 1,4-phenylenediacetonitrile + H2O
-
 Aspergillus luchuensis 1,4-phenylenediacetic acid + NH3
-
 ?
3.5.5.1 2-cyanophenyl acetonitrile + H2O
-
 Aspergillus luchuensis ?
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O
-
 Aspergillus luchuensis pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O
-
 Aspergillus luchuensis IFO 4308 pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + H2O
-
 Aspergillus luchuensis pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.1 4-cyanophenyl acetonitrile + H2O
-
 Aspergillus luchuensis ?
-
 ?
3.5.5.1 4-cyanopyridine + H2O
-
 Aspergillus luchuensis pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Aspergillus luchuensis benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O
-
 Aspergillus luchuensis IFO 4308 benzoic acid + NH3
-
 ?
3.5.5.1 fumaronitrile + H2O
-
 Aspergillus luchuensis fumaric acid + NH3
-
 ?
3.5.5.1 fumaronitrile + H2O
-
 Aspergillus luchuensis IFO 4308 fumaric acid + NH3
-
 ?
3.5.5.1 additional information no activity with: benzonitrile, (R,S)-mandelonitrile and Aspergillus luchuensis ?
-
-
3.5.5.1 additional information no activity with: benzonitrile, (R,S)-mandelonitrile and Aspergillus luchuensis IFO 4308 ?
-
-
3.5.5.1 phenylacetonitrile + 2 H2O
-
 Aspergillus luchuensis phenylacetate + NH3
-
 ?
3.5.5.1 phenylacetonitrile + 2 H2O
-
 Aspergillus luchuensis IFO 4308 phenylacetate + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 3 H2O
-
 Auricularia delicata mandelic acid + mandelamide + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 3 H2O
-
 Aspergillus luchuensis mandelic acid + mandelamide + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 3 H2O
-
 Macrophomina phaseolina mandelic acid + mandelamide + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 3 H2O
-
 Macrophomina phaseolina MS6 mandelic acid + mandelamide + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 3 H2O
-
 Auricularia delicata TFB-10046 SS5 mandelic acid + mandelamide + NH3
-
 ?
3.5.5.5 (R,S)-mandelonitrile + 3 H2O
-
 Aspergillus luchuensis IFO 4308 mandelic acid + mandelamide + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O
-
 Macrophomina phaseolina 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O
-
 Trichoderma virens Gv29-8 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O low activity Auricularia delicata 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O low activity Aspergillus luchuensis 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O
-
 Macrophomina phaseolina MS6 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,2-phenylenediacetonitrile + H2O low activity Aspergillus luchuensis IFO 4308 1,2-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,3-phenylenediacetonitrile + H2O
-
 Auricularia delicata 1,3-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,3-phenylenediacetonitrile + H2O
-
 Aspergillus luchuensis 1,3-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,3-phenylenediacetonitrile + H2O
-
 Trichoderma virens Gv29-8 1,3-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,3-phenylenediacetonitrile + H2O high activity Macrophomina phaseolina 1,3-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,4-phenylenediacetonitrile + H2O
-
 Auricularia delicata 1,4-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,4-phenylenediacetonitrile + H2O
-
 Aspergillus luchuensis 1,4-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,4-phenylenediacetonitrile + H2O
-
 Macrophomina phaseolina 1,4-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 1,4-phenylenediacetonitrile + H2O
-
 Trichoderma virens Gv29-8 1,4-phenylenediacetic acid + NH3
-
 ?
3.5.5.5 2-cyanophenyl acetonitrile + H2O
-
 Auricularia delicata ?
-
 ?
3.5.5.5 2-cyanophenyl acetonitrile + H2O
-
 Aspergillus luchuensis ?
-
 ?
3.5.5.5 2-cyanophenyl acetonitrile + H2O
-
 Macrophomina phaseolina ?
-
 ?
3.5.5.5 2-cyanopyridine + 2 H2O
-
 Trichoderma virens Gv29-8 pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.5 3-cyanopyridine + H2O
-
 Trichoderma virens Gv29-8 pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.5 4-cyanophenyl acetonitrile + H2O
-
 Auricularia delicata ?
-
 ?
3.5.5.5 4-cyanophenyl acetonitrile + H2O
-
 Aspergillus luchuensis ?
-
 ?
3.5.5.5 4-cyanophenyl acetonitrile + H2O high activity Macrophomina phaseolina ?
-
 ?
3.5.5.5 4-cyanopyridine + H2O
-
 Trichoderma virens Gv29-8 pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.5 4-cyanopyridine + H2O low activity Auricularia delicata pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.5 4-cyanopyridine + H2O low activity Auricularia delicata TFB-10046 SS5 pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.5 benzonitrile + 2 H2O
-
 Trichoderma virens Gv29-8 benzoic acid + NH3
-
 ?
3.5.5.5 cyanophenyl acetonitrile + H2O
-
 Trichoderma virens Gv29-8 ?
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Auricularia delicata fumaric acid + NH3
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Aspergillus luchuensis fumaric acid + NH3
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Macrophomina phaseolina fumaric acid + NH3
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Trichoderma virens Gv29-8 fumaric acid + NH3
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Macrophomina phaseolina MS6 fumaric acid + NH3
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Auricularia delicata TFB-10046 SS5 fumaric acid + NH3
-
 ?
3.5.5.5 fumaronitrile + H2O
-
 Aspergillus luchuensis IFO 4308 fumaric acid + NH3
-
 ?
3.5.5.5 additional information very low activity with: (R,S)-Mandelonitrile Trichoderma virens Gv29-8 ?
-
-
3.5.5.5 additional information very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine Auricularia delicata ?
-
-
3.5.5.5 additional information very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine Aspergillus luchuensis ?
-
-
3.5.5.5 additional information very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine Macrophomina phaseolina ?
-
-
3.5.5.5 additional information very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine Macrophomina phaseolina MS6 ?
-
-
3.5.5.5 additional information very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine Auricularia delicata TFB-10046 SS5 ?
-
-
3.5.5.5 additional information very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine Aspergillus luchuensis IFO 4308 ?
-
-
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Auricularia delicata phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Aspergillus luchuensis phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Macrophomina phaseolina phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Trichoderma virens Gv29-8 phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Macrophomina phaseolina MS6 phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Auricularia delicata TFB-10046 SS5 phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O
-
 Aspergillus luchuensis IFO 4308 phenylacetate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 NitAk1
-
 Aspergillus luchuensis
3.5.5.5 NitAd
-
 Auricularia delicata
3.5.5.5 NitAk2
-
 Aspergillus luchuensis
3.5.5.5 NitMp
-
 Macrophomina phaseolina
3.5.5.5 NitTv
-
 Trichoderma virens Gv29-8

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 30
-
 assay at Aspergillus luchuensis
3.5.5.5 30
-
 assay at Auricularia delicata
3.5.5.5 30
-
 assay at Aspergillus luchuensis
3.5.5.5 30
-
 assay at Macrophomina phaseolina
3.5.5.5 30
-
 assay at Trichoderma virens Gv29-8

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 8
-
 assay at Aspergillus luchuensis
3.5.5.5 8
-
 assay at Auricularia delicata
3.5.5.5 8
-
 assay at Aspergillus luchuensis
3.5.5.5 8
-
 assay at Macrophomina phaseolina
3.5.5.5 8
-
 assay at Trichoderma virens Gv29-8