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Literature summary extracted from

  • Shettigar, M.; Balotra, S.; Kasprzak, A.; Pearce, S.L.; Lacey, M.J.; Taylor, M.C.; Liu, J.W.; Cahill, D.; Oakeshott, J.G.; Pandey, G.
    Oxidative catabolism of (+)-pinoresinol is initiated by an unusual flavocytochrome encoded by translationally coupled genes within a cluster of (+)-pinoresinol-coinduced genes in Pseudomonas sp. strain SG-MS2 (2020), Appl. Environ. Microbiol., 86, e00375 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.9.2 expression in Escherichia coli Pseudomonas sp. SG-MS2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.9.2 0.00117
-
 (+)-pinoresinol pH 7.2, 28°C Pseudomonas sp. SG-MS2

Organism

EC Number Organism UniProt Comment Textmining
1.17.9.2 Pseudomonas sp. SG-MS2
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.9.2 28.2
-
 pH 7.2, 28°C Pseudomonas sp. SG-MS2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.9.2 (+)-pinoresinol + 2 oxidized azurin + H2O
-
 Pseudomonas sp. SG-MS2 (+)-6-hydroxypinoresinol + 2 reduced azurin + 2 H+
-
 ?
1.17.9.2 (+)-pinoresinol + 2,6-dichlorophenolindophenol + phenazine methosulfate + H2O
-
 Pseudomonas sp. SG-MS2 (+)-6-hydroxypinoresinol + reduced 2,6-dichlorophenolindophenol + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.9.2 BLX42_10690 gene name, flavoprotein component Pseudomonas sp. SG-MS2
1.17.9.2 BLX42_10695 gene name, cytochrome component Pseudomonas sp. SG-MS2
1.17.9.2 pinAB
-
 Pseudomonas sp. SG-MS2
1.17.9.2 pinoresinol alpha-hydroxylase
-
 Pseudomonas sp. SG-MS2

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.9.2 flavin the flavoprotein component has a covalently attached flavin that it is attached to H411 through an alpha-N3-histidyl-FAD linkage Pseudomonas sp. SG-MS2

Expression

EC Number Organism Comment Expression
1.17.9.2 Pseudomonas sp. SG-MS2 the enzyme is overexpressed upon exposure to (+)-pinoresinol, along with 45 other proteins up

General Information

EC Number General Information Comment Organism
1.17.9.2 physiological function the pinoresinol-oxidizing enzyme in Pseudomonas sp. SG-MS2 is a flavocytochrome complex produced by the translationally coupled BLX42_10690 and BLX42_10695 genes Pseudomonas sp. SG-MS2