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Literature summary extracted from

  • Han, R.; Ni, J.; Zhou, J.; Dong, J.; Xu, G.; Ni, Y.
    Engineering of cyclodextrin glycosyltransferase reveals pH-regulated mechanism of enhanced long-chain glycosylated sophoricoside specificity (2020), Appl. Environ. Microbiol., 86, e00004-20 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells Paenibacillus macerans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 A156V the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans
2.4.1.19 A156V/A166Y the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans
2.4.1.19 A156V/L174P the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans
2.4.1.19 A156V/L174P/A166Y the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans
2.4.1.19 A166Y the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans
2.4.1.19 A166Y/L174P the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans
2.4.1.19 L174P the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans P04830
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 Ni-NTA agarose column chromatography Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 genistein + maltodextrin less than 20% conversion ratio Paenibacillus macerans alpha-cyclodextrins
-
 ?
2.4.1.19 sophoricoside + maltodextrin more than 40% conversion ratio Paenibacillus macerans Glc-sophoricoside + Glc2-sophoricoside + Glc3-sophoricoside + Glc4-sophoricoside + Glc5-sophoricoside + Glc6-sophoricoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 75000, SDS-PAGE Paenibacillus macerans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Paenibacillus macerans
2.4.1.19 cyclodextrin glucosyltransferase
-
 Paenibacillus macerans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.19 0.063
-
 sophoricoside wild type enzyme, at pH 6.0 and 50°C Paenibacillus macerans
2.4.1.19 0.063
-
 sophoricoside mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C Paenibacillus macerans
2.4.1.19 0.079
-
 maltodextrin wild type enzyme, at pH 6.0 and 50°C Paenibacillus macerans
2.4.1.19 0.079
-
 maltodextrin mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C Paenibacillus macerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5
-
-
 Paenibacillus macerans