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Literature summary extracted from

  • Buryska, T.; Babkova, P.; Vavra, O.; Damborsky, J.; Prokop, Z.
    A haloalkane dehalogenase from a marine microbial consortium possessing exceptionally broad substrate specificity (2018), Appl. Environ. Microbiol., 84, e01684-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 expressed in Escherichia coli BL21(DE3) Lyngbya majuscula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 0.38
-
 1-Chlorobutane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.43
-
 1-Iodobutane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.86
-
 1-Iodopropane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.9
-
 4-bromobutyronitrile 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 2.3
-
 1,2-Dibromoethane 37°C, pH 8.6 Lyngbya majuscula

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Lyngbya majuscula Q6DND9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 metallo-affinity chromatography Lyngbya majuscula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,2,3-tribromopropane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2,3-dibromo-1-propanol + bromide
-
 ?
3.8.1.5 1,2-dibromo-3-chloropropane about 35% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-bromo-3-chloro-1-propanol + bromide
-
 ?
3.8.1.5 1,2-dibromoethane + H2O about 45% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-bromoethanol + bromide
-
 ?
3.8.1.5 1,2-dibromopropane + H2O about 15% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-dibromobutane + H2O about 20% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 3-bromo-1-propanol + bromide
-
 ?
3.8.1.5 1,3-diiodopropane + H2O about 25% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 3-iodo-1-propanol + iodide
-
 ?
3.8.1.5 1-bromo-2-chloroethane about 50% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 2-chloro-1-ethanol + bromide
-
 ?
3.8.1.5 1-bromo-3-chloropropane + H2O about 25% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 3-chloro-1-propanol + bromide
-
 ?
3.8.1.5 1-bromobutane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-butanol + bromide
-
 ?
3.8.1.5 1-chlorobutane + H2O less than 5% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-butanol + chloride
-
 ?
3.8.1.5 1-iodobutane + H2O about 5% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-butanol + iodide
-
 ?
3.8.1.5 1-iodopropane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula 1-propanol + iodide
-
 ?
3.8.1.5 2,3-dichloroprop-1-ene + H2O about 15% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula ?
-
 ?
3.8.1.5 3-chloro-2-methylprop-1-ene + H2O about 5% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula ?
-
 ?
3.8.1.5 4-bromobutyronitrile + H2O best substrate Lyngbya majuscula 4-hydroxybutyronitrile + bromide
-
 ?
3.8.1.5 bromocyclohexane + H2O about 20% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula cyclohexanol + bromide
-
 ?
3.8.1.5 chlorocyclopentane + H2O about 10% of the activity compared to 4-bromobutyronitrile Lyngbya majuscula cyclopentanol + chloride
-
 ?
3.8.1.5 additional information the enzyme prefers brominated substrates over chlorinated and iodinated substrates. Moderate to high enantioselectivity with a preference for the (R) enantiomer is observed for brominated esters with the highest E value of 106 achieved with ethyl-2-bromopropionate. Negligible enantioselectivity is observed with either of the tested brominated n-alkanes Lyngbya majuscula ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DmmA
-
 Lyngbya majuscula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.5 40
-
-
 Lyngbya majuscula

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.5 20 45 20°C: about 30% of maximal activity, 45°C: about 40% of maximal activity Lyngbya majuscula

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.5 45
-
 half-life: 3.0 h Lyngbya majuscula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.5 0.17
-
 1-Chlorobutane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.21
-
 1-Iodobutane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.43
-
 1-Iodopropane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 1.2
-
 4-bromobutyronitrile 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 1.8
-
 1,2-Dibromoethane 37°C, pH 8.6 Lyngbya majuscula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 8.7
-
-
 Lyngbya majuscula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.5 7.5 9.2 at least 90% of maximal activity within a pH range of 7.5 to 9.2 and more than 20% at pH values of 6.2 to 11.5 Lyngbya majuscula

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8.1.5 0.45
-
 1-Chlorobutane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.49
-
 1-Iodobutane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.5
-
 1-Iodopropane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 0.8
-
 1,2-Dibromoethane 37°C, pH 8.6 Lyngbya majuscula
3.8.1.5 1.3
-
 4-bromobutyronitrile 37°C, pH 8.6 Lyngbya majuscula