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Literature summary extracted from

  • Roettig, A.; Strittmatter, C.S.; Schauer, J.; Hiessl, S.; Poehlein, A.; Daniel, R.; Steinbuechel, A.
    Role of wax ester synthase/acyl coenzyme A diacylglycerol acyltransferase in oleaginous Streptomyces sp. strain G25 (2016), Appl. Environ. Microbiol., 82, 5969-5981 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene atfG25, DNA and amino acid sequence determination and analysis, geneomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3) Streptomyces sp. G25
2.3.1.75 expressed in Escherichia coli C41(DE3) cells Streptomyces sp. G25
2.3.1.75 gene atfG25, DNA and amino acid sequence determination and analysis, genomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3) Streptomyces sp. G25

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information generation of an atfG25OMEGAApr insertion mutant of Streptomyces sp. G25 Streptomyces sp. G25
2.3.1.75 additional information generation of an atfG25OMEGAApr insertion mutant of Streptomyces sp. G25 Streptomyces sp. G25

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Streptomyces sp. G25
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.75 acyl-CoA + a long-chain alcohol Streptomyces sp. G25
-
 CoA + a long-chain ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Streptomyces sp. G25
-
-
-
2.3.1.75 Streptomyces sp. G25
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by ultracentrifugation and nickel affinity chromatography Streptomyces sp. G25
2.3.1.75 HisTrap column chromatography and Superdex S200 gel filtration Streptomyces sp. G25
2.3.1.75 recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by ultracentrifugation and nickel affinity chromatography Streptomyces sp. G25

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 additional information Streptomyces strain G25 can accumulate extraordinarily large amounts of triacylglycerol (TAG) and consists of 64% fatty acids (dry weight) when cultivated with glucose and 50% fatty acids (dry weight) when cultivated with cellobiose Streptomyces sp. G25
-
2.3.1.75 additional information Streptomyces strain G25 can accumulate extraordinarily large amounts of triacylglycerol (TAG) and consists of 64% fatty acids (dry weight) when cultivated with glucose and 50% fatty acids (dry weight) when cultivated with cellobiose Streptomyces sp. G25
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Streptomyces sp. G25 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 lauryl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Streptomyces sp. G25 CoA + 1,2-dipalmitoyl-3-lauryl-glycerol
-
 ?
2.3.1.20 additional information the purified enzyme AtfG25 shows acyltransferase activity with C12- or C16-acyl-CoA, C12 to C18 alcohols (ethanol, butanol, hexanol, octanol, decanol, dodecanol, tetradecanol, hexadecanol, palmitoleyl alcohol, and octadecanol, cf. EC 2.3.1.75), and dipalmitoyl glycerol. Substrate specificity, overview. The DGAT activity of AtfG25 corresponds to about 70% of its wax synthase activity Streptomyces sp. G25 ?
-
-
2.3.1.20 palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Streptomyces sp. G25 CoA + 1,2,3-tripalmitoylglycerol
-
 ?
2.3.1.75 acyl-CoA + a long-chain alcohol
-
 Streptomyces sp. G25 CoA + a long-chain ester
-
 ?
2.3.1.75 lauroyl-CoA + hexadecanol high activity Streptomyces sp. G25 hexadecanyl laurate + CoA
-
 ?
2.3.1.75 lauryl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Streptomyces sp. G25 ?
-
 ?
2.3.1.75 additional information the purified enzyme AtfG25 shows acyltransferase activity with C12- or C16-acyl-CoA (cf. EC 2.3.1.20), C2 to C18 alcohols (ethanol, butanol, hexanol, octanol, decanol, dodecanol, tetradecanol, hexadecanol, palmitoleyl alcohol, and octadecanol), and dipalmitoyl glycerol. Substrate specificity, overview. The DGAT activity of AtfG25 corresponds to about 70% of itsWS activity Streptomyces sp. G25 ?
-
-
2.3.1.75 palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol high activity Streptomyces sp. G25 ?
-
 ?
2.3.1.75 palmitoyl-CoA + 1-hexadecanol highest activity Streptomyces sp. G25 palmitic acid hexadecyl ester + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + dodecanol
-
 Streptomyces sp. G25 dodecyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + dodecanol
-
 Streptomyces sp. G25 CoA + dodecyl palmitate
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecanol
-
 Streptomyces sp. G25 CoA + hexadecyl palmitate
-
 ?
2.3.1.75 palmitoyl-CoA + octadecanol lowest activity Streptomyces sp. G25 octadecyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + octadecanol
-
 Streptomyces sp. G25 CoA + octadecyl palmitate
-
 ?
2.3.1.75 palmitoyl-CoA + palmitoleyl alcohol
-
 Streptomyces sp. G25 ?
-
 ?
2.3.1.75 palmitoyl-CoA + tetradecanol high activity Streptomyces sp. G25 tetradecyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + tetradecanol
-
 Streptomyces sp. G25 CoA + tetradecyl palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.75 ? x * 48740, calculated from amino acid sequence Streptomyces sp. G25

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 AtfG25
-
 Streptomyces sp. G25
2.3.1.20 More see also EC 2.3.1.75 Streptomyces sp. G25
2.3.1.20 wax ester synthase/acyl CoA:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
2.3.1.20 wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
2.3.1.20 WS/DGAT
-
 Streptomyces sp. G25
2.3.1.75 ATF
-
 Streptomyces sp. G25
2.3.1.75 AtfG25
-
 Streptomyces sp. G25
2.3.1.75 More see also EC 2.3.1.20 Streptomyces sp. G25
2.3.1.75 wax ester synthase/acyl CoA:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
2.3.1.75 wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Streptomyces sp. G25
2.3.1.75 WS/DGAT
-
 Streptomyces sp. G25

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 37
-
 assay at Streptomyces sp. G25
2.3.1.75 37
-
 assay at Streptomyces sp. G25

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.4
-
 assay at Streptomyces sp. G25
2.3.1.75 7.4
-
 assay at Streptomyces sp. G25

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.75 Streptomyces sp. G25 calculated from amino acid sequence
-
 9.53

General Information

EC Number General Information Comment Organism
2.3.1.20 malfunction the respective gene, atfG25, is inactivated in Streptomyces sp. strain G25, cells of the insertion mutant still exhibit DGAT activity and are able to store TAG, albeit in lower quantities and at lower rates than the wild-type strain Streptomyces sp. G25
2.3.1.20 metabolism the final step in triacylglycerol (TAG) biosynthesis is catalyzed by diacylglycerol acyltransferase (DGAT) Streptomyces sp. G25
2.3.1.20 physiological function enzyme AtfG25 has an important, but not exclusive, role in triacylglycerol (TAG) biosynthesis in the Streptomyces sp. G25 isolate, suggesting the presence of alternative metabolic pathways for lipid accumulation Streptomyces sp. G25
2.3.1.75 evolution distribution of putative WS/DGAT in Streptomyces genomes, overview Streptomyces sp. G25
2.3.1.75 malfunction the respective gene, atfG25, is inactivated in Streptomyces sp. strain G25, cells of the insertion mutant still exhibit DGAT activity and are able to store TAG, albeit in lower quantities and at lower rates than the wild-type strain Streptomyces sp. G25
2.3.1.75 physiological function enzyme AtfG25 has an important, but not exclusive, role in triacylglycerol (TAG) biosynthesis in the Streptomyces sp. G25 isolate, suggesting the presence of alternative metabolic pathways for lipid accumulation Streptomyces sp. G25