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Literature summary extracted from

  • Ishiyama, D.; Vujaklija, D.; Davies, J.
    Novel pathway of salicylate degradation by Streptomyces sp. strain WA46 (2004), Appl. Environ. Microbiol., 70, 1297-1306 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.65 expression in Escherichia coli Streptomyces sp. WA46

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.65 (2-hydroxybenzoyl)adenylate + CoA Streptomyces sp. WA46
-
 AMP + 2-hydroxybenzoyl-CoA
-
 ?
6.2.1.65 ATP + salicylate Streptomyces sp. WA46
-
 diphosphate + (2-hydroxybenzoyl)adenylate
-
 ?
6.2.1.65 ATP + salicylate + CoA Streptomyces sp. WA46
-
 AMP + diphosphate + 2-hydroxybenzoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.65 Streptomyces sp. WA46 Q7X279
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.65 (2-hydroxybenzoyl)adenylate + CoA
-
 Streptomyces sp. WA46 AMP + 2-hydroxybenzoyl-CoA
-
 ?
6.2.1.65 ATP + salicylate
-
 Streptomyces sp. WA46 diphosphate + (2-hydroxybenzoyl)adenylate
-
 ?
6.2.1.65 ATP + salicylate + CoA
-
 Streptomyces sp. WA46 AMP + diphosphate + 2-hydroxybenzoyl-CoA
-
 ?
6.2.1.65 additional information no substrates: benzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, gentisate, 2-aminobenzoate, salicylamide, salicylaldoxime, and 2-hydroxyphenyl acetate Streptomyces sp. WA46 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
6.2.1.65 salicylyl-CoA synthase/salicylate adenylyltransferase
-
 Streptomyces sp. WA46
6.2.1.65 sdgA
-
 Streptomyces sp. WA46

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.65 CoA
-
 Streptomyces sp. WA46

General Information

EC Number General Information Comment Organism
6.2.1.65 physiological function disruption of the SdgA gene blocks salicylate degradation. Cell extracts from Escherichia coli transformants expressing each of the SdgABCDEFGHR genes show that SdgA, SdgC, ATP, CoA, and NADH are necessary to convert salicylate to gentisate. SdgA requires CoA and ATP as cofactors Streptomyces sp. WA46