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Literature summary extracted from

  • Novikov, A.; Riabchenko, L.; Leonova, T.; Larikova, G.; Lavrov, K.; Glinskii, S.; Yanenko, A.
    Bacterial strain Alcaligenes denitrificans C-32 containing two nitrilases with different substrate specificities (2017), Appl. Biochem. Microbiol., 53, 786-791 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.7 expression in Escherichia coli Achromobacter denitrificans

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.7 Achromobacter denitrificans A0A286S9Z8
-
-
3.5.5.7 Achromobacter denitrificans A0A291PNG0
-
-
3.5.5.7 Achromobacter denitrificans C-32 A0A286S9Z8
-
-
3.5.5.7 Achromobacter denitrificans C-32 A0A291PNG0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.7 acrylonitrile + 2 H2O
-
Achromobacter denitrificans acrylic acid + NH3
-
?
3.5.5.7 acrylonitrile + 2 H2O acrylonitrile is the best substrates for nitC1-encoded nitrilase Achromobacter denitrificans acrylic acid + NH3
-
?
3.5.5.7 acrylonitrile + 2 H2O acrylonitrile is the best substrates for nitC1-encoded nitrilase Achromobacter denitrificans C-32 acrylic acid + NH3
-
?
3.5.5.7 acrylonitrile + 2 H2O
-
Achromobacter denitrificans C-32 acrylic acid + NH3
-
?
3.5.5.7 adiponitrile + 4 H2O 42% of the activity as compared to acrylonitrile Achromobacter denitrificans adipic acid + 2 NH3
-
?
3.5.5.7 adiponitrile + 4 H2O activity is comparable to activity with acrylonitrile Achromobacter denitrificans adipic acid + 2 NH3
-
?
3.5.5.7 adiponitrile + 4 H2O 42% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 adipic acid + 2 NH3
-
?
3.5.5.7 adiponitrile + 4 H2O activity is comparable to activity with acrylonitrile Achromobacter denitrificans C-32 adipic acid + 2 NH3
-
?
3.5.5.7 benzonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans benzoic acid + NH3
-
?
3.5.5.7 benzonitrile + 2 H2O 8% of the activity as compared to acrylonitrile Achromobacter denitrificans benzoic acid + NH3
-
?
3.5.5.7 benzonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 benzoic acid + NH3
-
?
3.5.5.7 benzonitrile + 2 H2O 8% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 benzoic acid + NH3
-
?
3.5.5.7 butyronitrile + 2 H2O 75% of the activity as compared to acrylonitrile Achromobacter denitrificans butyric acid + NH3
-
?
3.5.5.7 butyronitrile + 2 H2O 83% of the activity as compared to acrylonitrile Achromobacter denitrificans butyric acid + NH3
-
?
3.5.5.7 butyronitrile + 2 H2O 75% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 butyric acid + NH3
-
?
3.5.5.7 butyronitrile + 2 H2O 83% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 butyric acid + NH3
-
?
3.5.5.7 phenylacetonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans phenylacetic acid + NH3
-
?
3.5.5.7 phenylacetonitrile + 2 H2O best substrate for nitC2-encoded nitrilase. Activity is 16.7fold higher as compared to activity with acrylonitrile Achromobacter denitrificans phenylacetic acid + NH3
-
?
3.5.5.7 phenylacetonitrile + 2 H2O 5% of the activity as compared to acrylonitrile Achromobacter denitrificans C-32 phenylacetic acid + NH3
-
?
3.5.5.7 phenylacetonitrile + 2 H2O best substrate for nitC2-encoded nitrilase. Activity is 16.7fold higher as compared to activity with acrylonitrile Achromobacter denitrificans C-32 phenylacetic acid + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.7 nitC1
-
Achromobacter denitrificans
3.5.5.7 nitC2
-
Achromobacter denitrificans