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Literature summary extracted from

  • Ban, X.; Li, C.; Zhang, Y.; Gu, Z.; Cheng, L.; Hong, Y.; Li, Z.
    Importance of C-terminal extension in thermophilic 1,4-alpha-glucan branching enzyme from Geobacillus thermoglucosidans STB02 (2020), Appl. Biochem. Biotechnol., 190, 1010-1022 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 expressed in Escherichia coli BL21(DE3) cells Parageobacillus thermoglucosidasius

General Stability

EC Number General Stability Organism
2.4.1.18 truncation of the C-terminal extension results in greater stability and solubility than the wild type, as well as a lower sensitivity to the presence of added metal ions Parageobacillus thermoglucosidasius

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.18 Al3+ the wild type enzyme shows bout 75% residual activity at 10 mM. The C-terminally truncated enzyme shows about 95% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Ca2+ the wild type enzyme shows about 90% residual activity at 10 mM. The C-terminally truncated enzyme shows about 97% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Co2+ the wild type enzyme shows complete inhibition at 3 mM. The C-terminally truncated enzyme shows about 28% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Cr2+ the wild type enzyme shows about 75% residual activity at 10 mM. The C-terminally truncated enzyme shows about 94% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Cu2+ the wild type enzyme shows about 10% residual activity at 10 mM. The C-terminally truncated enzyme shows about 51% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Fe2+ the wild type enzyme shows about 85% residual activity at 10 mM. The C-terminally truncated enzyme shows about 97% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Fe3+ the wild type enzyme shows about 78% residual activity at 10 mM. The C-terminally truncated enzyme shows about 95% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Mn2+ the wild type enzyme shows about 35% residual activity at 10 mM. The C-terminally truncated enzyme shows about 69% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Ni2+ the wild type enzyme shows about 2% residual activity at 10 mM. The C-terminally truncated enzyme shows about 25% residual activity at 10 mM Parageobacillus thermoglucosidasius
2.4.1.18 Zn2+ the wild type enzyme shows about 18% residual activity at 10 mM. The C-terminally truncated enzyme shows about 58% residual activity at 10 mM Parageobacillus thermoglucosidasius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.18 Ba2+ the wild type enzyme shows 100% activity at 1 mM. The C-terminally truncated enzyme shows about 102% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.18 Ca2+ the C-terminally truncated enzyme shows about 105% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.18 K+ the wild type enzyme shows about 128% activity at 1 mM. The C-terminally truncated enzyme shows about 103% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.18 Mg2+ the wild type and C-terminally truncated enzyme show 100% activity at 1 mM Parageobacillus thermoglucosidasius
2.4.1.18 Na+ the wild type enzyme shows about 118% activity at 1 mM. The C-terminally truncated enzyme shows about 106% activity at 1 mM Parageobacillus thermoglucosidasius

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Parageobacillus thermoglucosidasius A0A068F9H3
-
-
2.4.1.18 Parageobacillus thermoglucosidasius STB02 A0A068F9H3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 HiTrap ANX column chromatography and HiTrap phenyl column chromatography Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 amylopectin
-
 Parageobacillus thermoglucosidasius ?
-
 ?
2.4.1.18 amylopectin
-
 Parageobacillus thermoglucosidasius STB02 ?
-
 ?
2.4.1.18 amylose
-
 Parageobacillus thermoglucosidasius ?
-
 ?
2.4.1.18 amylose
-
 Parageobacillus thermoglucosidasius STB02 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 GBE
-
 Parageobacillus thermoglucosidasius