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Literature summary extracted from

  • Feng, Y.; Zhang, Y.; Ding, W.; Wu, P.; Cao, X.; Xue, S.
    Expanding of phospholipid diacylglycerol acyltransferase (PDAT) from Saccharomyces cerevisiae as multifunctional biocatalyst with broad acyl donor/acceptor selectivity (2019), Appl. Biochem. Biotechnol., 188, 824-835 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.158 expressed in Pichia pastoris Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.158 65000
-
 deglycosylated enzyme, SDS-PAGE Saccharomyces cerevisiae
2.3.1.158 95000
-
 glycosylated enzyme, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.158 Saccharomyces cerevisiae P40345
-
-
2.3.1.158 Saccharomyces cerevisiae ATCC 204508 P40345
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.158 glycoprotein four glycosylation sites, the degree of glycosylation site N469 is the largest Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.158
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol the enzyme is capable of using broad acyl donors such as PA, PS, PG, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and acyl-CoA. The enzyme is more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, the enzyme prefers 1,2 to 1,3-diacylglycerol, while 12:0/12:0 1,3-diacylglycerol is identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 1,3-diacylglycerol Saccharomyces cerevisiae lysophospholipid + triacylglycerol
-
 ?
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol the enzyme is capable of using broad acyl donors such as PA, PS, PG, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and acyl-CoA. The enzyme is more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, the enzyme prefers 1,2 to 1,3-diacylglycerol, while 12:0/12:0 1,3-diacylglycerol is identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 1,3-diacylglycerol Saccharomyces cerevisiae ATCC 204508 lysophospholipid + triacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.158 PDAT
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.158 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.158 7.4
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.3.1.158 metabolism the enzyme is responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, catalyzing membrane lipid transformation Saccharomyces cerevisiae