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Literature summary extracted from
- Synthesis of Lactosucrose Using a Recombinant Levansucrase from Brenneria goodwinii (2018), Appl. Biochem. Biotechnol., 186, 292-305 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.10 | expressed in Escherichia coli BL21(DE3) cells | Brenneria goodwinii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.10 | Brenneria goodwinii | A0A0G4JRN2 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.10 | additional information | the enzyme also hydrolyzes sucrose to D-glucose and D-fructose | Brenneria goodwinii | ? | - |
- |
 |
| 2.4.1.10 | sucrose + lactose | the production condition is optimized as pH at 6.0, temperature at 35°C, 5 units/ml enzyme, 180 g/l sucrose, and 180 g/l lactose | Brenneria goodwinii | lactosucrose + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.10 | ? | x * 49000, SDS-PAGE | Brenneria goodwinii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.10 | 35 | - |
- |
Brenneria goodwinii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.10 | 6 | - |
- |
Brenneria goodwinii |
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Release 2023.1 (January 2023)
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