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Literature summary extracted from

  • Mueller, C.; Ortmann, T.; Abi, A.; Hartig, D.; Scholl, S.; Joerdening, H.J.
    Immobilization and characterization of E. gracilis extract with enriched laminaribiose phosphorylase activity for bienzymatic production of laminaribiose (2017), Appl. Biochem. Biotechnol., 182, 197-215 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.31 immobilization on Sepabeads EC-EP/S results in 65% enzyme activity. The immobilized enzyme loses 48% of its activity when used 7 times. Laminaribiose phosphorylase that is encapsulated in chitosan or bentonite retains 75% activity Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose Euglena gracilis
-
 laminaribiose + phosphate
-
 ?
2.4.1.31 laminaribiose + alpha-D-glucose 1-phosphate Euglena gracilis
-
 laminaritriose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 Euglena gracilis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
2.4.1.31 20°C, 0.05 M sodium phosphate buffer (pH 6.3), 89 days, 84% loss of activity Euglena gracilis
2.4.1.31 4°C, 0.05 M sodium phosphate buffer (pH 6.3), 89 days, 48% loss of activity Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose
-
 Euglena gracilis laminaribiose + phosphate
-
 ?
2.4.1.31 laminaribiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaritriose + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.31 37
-
 the half-life of the native enzyme at 37°C is 8.7 h. The half-life of the immobilized enzyme is 8.9 h Euglena gracilis