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Literature summary extracted from

  • Andi, B.; Soares, A.S.; Shi, W.; Fuchs, M.R.; McSweeney, S.; Liu, Q.
    Structure of the dihydrolipoamide succinyltransferase catalytic domain from Escherichia coli in a novel crystal form a tale of a common protein crystallization contaminant (2019), Acta Crystallogr. Sect. F, 75, 616-624 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.61 expressed in Escherichia coli BL21 (DE3) pLysS cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.61 catalytic domain, hanging drop vapor diffusion method, using 1 M sodium acetate, 50 mM cadmium sulfate, 50 mM HEPES pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.61 succinyl-CoA + enzyme N6-(dihydrolipoyl)lysine Escherichia coli
-
 CoA + enzyme N6-(S-succinyldihydrolipoyl)lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.61 Escherichia coli P0AFG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.61 Ni-NTA resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.61 succinyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
 Escherichia coli CoA + enzyme N6-(S-succinyldihydrolipoyl)lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.61 ? x * 44000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.61 dihydrolipoamide succinyltransferase
-
 Escherichia coli
2.3.1.61 DSCD catalytic enzyme domain Escherichia coli