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Literature summary extracted from

  • Stellmacher, L.; Sandalova, T.; Schneider, S.; Schneider, G.; Sprenger, G.A.; Samland, A.K.
    Novel mode of inhibition by D-tagatose 6-phosphate through a Heyns rearrangement in the active site of transaldolase B variants (2016), Acta Crystallogr. Sect. D, 72, 467-476 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.2 expressed in Escherichia coli BL21 (DE3) Star pLysS cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.2 mutant enzyme F178Y with bound D-tagatose 6-phosphate , hanging drop vapor diffusion method, using 0.15 M Li2SO4, 18% (w/v) PEG 3350. Mutant enzyme E96Q with bound D-fructose 6-phosphate, hanging drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 18% (w/v) PEG3350 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.2 E96Q the mutant enzyme with reduced transaldolase activity is irreversibly inhibited by D-tagatose 6-phosphate Escherichia coli
2.2.1.2 E96Q/F178Y the mutant enzyme is irreversibly inhibited by D-tagatose 6-phosphate Escherichia coli
2.2.1.2 F178Y the mutant enzyme is inhibited by D-tagatose 6-phosphate Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.2 additional information the wild type enzyme is not inhibited by D-tagatose 6-phosphate Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate Escherichia coli
-
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
2.2.1.2 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate Escherichia coli
-
D-erythrose 4-phosphate + D-fructose 6-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Escherichia coli P0A870
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate
-
Escherichia coli sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
r
2.2.1.2 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
Escherichia coli D-erythrose 4-phosphate + D-fructose 6-phosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.2.1.2 TALB
-
Escherichia coli
2.2.1.2 transaldolase B
-
Escherichia coli