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Literature summary extracted from

  • Xu, Z.; Stogios, P.J.; Quaile, A.T.; Forsberg, K.J.; Patel, S.; Skarina, T.; Houliston, S.; Arrowsmith, C.; Dantas, G.; Savchenko, A.
    Structural and functional survey of environmental aminoglycoside acetyltransferases reveals functionality of resistance enzymes (2017), ACS Infect. Dis., 3, 653-665 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.60 expressed in Escherichia coli BL21(DE3)-Gold cells uncultured bacterium
2.3.1.60 expressed in Escherichia coli BL21(DE3)-Gold cells Pseudomonas aeruginosa
2.3.1.82 expressed in Escherichia coli BL21(DE3)-Gold cells uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.60 apoenzyme, sitting drop vapor diffusion method, using 40% (w/v) PEG 300 and phosphate-citrate (pH 5.2) Pseudomonas aeruginosa
2.3.1.60 in complex with CoA, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M magnesium formate uncultured bacterium
2.3.1.82 in complex with sisomicin uncultured bacterium
2.3.1.82 meta-AAC0020 enzyme mutant Y138A in complex with sisomicin, sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 0.2 M calcium acetate. The apoenzyme is crystallized in a solution containing 4 M sodium formate uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.82 Y138A catalytically inactive uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.021
-
 acetyl-CoA K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C uncultured bacterium
2.3.1.82 0.022
-
 gentamycin K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C uncultured bacterium
2.3.1.82 0.028
-
 tobramycin K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C uncultured bacterium
2.3.1.82 0.038
-
 sisomicin K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C uncultured bacterium
2.3.1.82 0.346
-
 kanamycin K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.60 Pseudomonas aeruginosa Q51407
-
-
2.3.1.60 uncultured bacterium
-
-
-
2.3.1.60 uncultured bacterium A0A059WV44
-
-
2.3.1.82 uncultured bacterium A0A059WZ16
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.60 Ni-NTA column chromatography and Superdex S200 gel filtration uncultured bacterium
2.3.1.60 Ni-NTA column chromatography and Superdex S200 gel filtration Pseudomonas aeruginosa
2.3.1.82 Ni-NTA column chromatography and Superdex S200 gel filtration uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.60 acetyl-CoA + gentamicin
-
 uncultured bacterium CoA + N3-acetylgentamicin
-
 ?
2.3.1.60 acetyl-CoA + gentamicin
-
 Pseudomonas aeruginosa CoA + N3-acetylgentamicin
-
 ?
2.3.1.60 acetyl-CoA + kanamycin
-
 uncultured bacterium CoA + N3''-acetylkanamycin
-
 ?
2.3.1.60 acetyl-CoA + kanamycin
-
 Pseudomonas aeruginosa CoA + N3''-acetylkanamycin
-
 ?
2.3.1.60 acetyl-CoA + sisomicin
-
 uncultured bacterium CoA + N3-acetylsisomicin
-
 ?
2.3.1.60 acetyl-CoA + sisomicin
-
 Pseudomonas aeruginosa CoA + N3-acetylsisomicin
-
 ?
2.3.1.60 acetyl-CoA + tobramycin
-
 uncultured bacterium CoA + N3-acetyltobramycin
-
 ?
2.3.1.60 acetyl-CoA + tobramycin
-
 Pseudomonas aeruginosa CoA + N3-acetyltobramycin
-
 ?
2.3.1.82 acetyl-CoA + gentamycin
-
 uncultured bacterium CoA + ?
-
 ?
2.3.1.82 acetyl-CoA + kanamycin
-
 uncultured bacterium CoA + 6'-N-acetylkanamycin
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 uncultured bacterium CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + kanamycin C
-
 uncultured bacterium CoA + N6'-acetylkanamycin C
-
 ?
2.3.1.82 acetyl-CoA + sisomicin
-
 uncultured bacterium CoA + N6'-acetylsisomicin
-
 ?
2.3.1.82 acetyl-CoA + sisomicin
-
 uncultured bacterium CoA + 6'-N-acetylsisomicin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 uncultured bacterium CoA + N6'-acetyltobramycin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 uncultured bacterium CoA + 6'-N-acetyltobramycin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.60 AAC
-
 uncultured bacterium
2.3.1.60 AAC
-
 Pseudomonas aeruginosa
2.3.1.60 AAC(3)-Ib
-
 Pseudomonas aeruginosa
2.3.1.60 aminoglycoside acetyltransferase
-
 uncultured bacterium
2.3.1.60 aminoglycoside acetyltransferase
-
 Pseudomonas aeruginosa
2.3.1.60 aminoglycoside N-acetyltransferase
-
 uncultured bacterium
2.3.1.60 aminoglycoside N-acetyltransferase
-
 Pseudomonas aeruginosa
2.3.1.60 meta-AAC0005
-
 uncultured bacterium
2.3.1.60 meta-AAC0007
-
 uncultured bacterium
2.3.1.60 meta-AAC0012
-
 uncultured bacterium
2.3.1.82 AAC
-
 uncultured bacterium
2.3.1.82 AAC(6')
-
 uncultured bacterium
2.3.1.82 AAC3-I
-
 uncultured bacterium
2.3.1.82 aminoglycoside acetyltransferase
-
 uncultured bacterium
2.3.1.82 aminoglycoside N-acetyltransferase
-
 uncultured bacterium
2.3.1.82 meta-AAC0020
-
 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 0.207
-
 kanamycin pH 7.5, 25°C uncultured bacterium
2.3.1.82 1.17
-
 gentamycin pH 7.5, 25°C uncultured bacterium
2.3.1.82 1.2
-
 tobramycin pH 7.5, 25°C uncultured bacterium
2.3.1.82 1.51
-
 acetyl-CoA pH 7.5, 25°C uncultured bacterium
2.3.1.82 2.42
-
 sisomicin pH 7.5, 25°C uncultured bacterium

General Information

EC Number General Information Comment Organism
2.3.1.60 physiological function the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin uncultured bacterium
2.3.1.60 physiological function the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin Pseudomonas aeruginosa
2.3.1.82 physiological function the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.82 0.6
-
 kanamycin K 0.5 value, Hill coefficient 0.62, pH 7.5, 25°C uncultured bacterium
2.3.1.82 43
-
 tobramycin K 0.5 value, Hill coefficient 2.3, pH 7.5, 25°C uncultured bacterium
2.3.1.82 52
-
 gentamycin K 0.5 value, Hill coefficient 3.14, pH 7.5, 25°C uncultured bacterium
2.3.1.82 64
-
 sisomicin K 0.5 value, Hill coefficient 4.39, pH 7.5, 25°C uncultured bacterium
2.3.1.82 69
-
 acetyl-CoA K 0.5 value, Hill coefficient 1.77, pH 7.5, 25°C uncultured bacterium