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Literature summary extracted from

  • Cho, S.H.; Kim, S.Y.; Tomita, T.; Shiraishi, T.; Park, J.S.; Sato, S.; Kudo, F.; Eguchi, T.; Funa, N.; Nishiyama, M.; Kuzuyama, T.
    Fosfomycin biosynthesis via transient cytidylylation of 2-hydroxyethylphosphonate by the bifunctional Fom1 enzyme (2017), ACS Chem. Biol., 12, 2209-2215 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.104 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and B834 (DE3) cells Streptomyces wedmorensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.104 native enzyme in complex with cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate], hanging drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.5) and 4.5 M NaCl Streptomyces wedmorensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.104 Co2+ required for activity. 5 mM used in assay conditions Streptomyces wedmorensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.104 2-hydroxyethylphosphonate + CTP Streptomyces wedmorensis
-
 cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate] + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.104 Streptomyces wedmorensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.104 Ni2+-affinity column chromatography Streptomyces wedmorensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.104 2-hydroxyethylphosphonate + CTP
-
 Streptomyces wedmorensis cytidine 5'-[[hydroxy(2-hydroxyethyl)phosphonoyl]phosphate] + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.104 Fom1 Fom1 contains a cytidylyltransferase domain at its N-terminus Streptomyces wedmorensis
2.7.7.104 Fom1CyTase
-
 Streptomyces wedmorensis