EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.292 | gene dnrK, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces peucetius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.292 | 0.106 | - |
2-chloro-4-nitrophenol | pH 8.0, 37°C | Streptomyces peucetius | |
2.1.1.292 | 0.138 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Streptomyces peucetius | |
2.1.1.292 | 0.335 | - |
S-adenosyl-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium | pH 8.0, 37°C | Streptomyces peucetius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.292 | S-adenosyl-L-methionine + carminomycin | Streptomyces peucetius | - |
S-adenosyl-L-homocysteine + daunorubicin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.292 | Streptomyces peucetius | Q06528 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.292 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Streptomyces peucetius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.292 | additional information | the enzyme has been reported to methylate various modified anthracyclines and flavonoids as surrogate substrates. 2-Chloro-4-nitrophenol is a substrate of DnrK. High-throughput DnrK colorimetric assay with the surrogate acceptor 2-chloro-4-nitrophenol and the different cosubstrates. No activity with derivative S-(7-deazaadenosyl)-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium | Streptomyces peucetius | ? | - |
- |
|
2.1.1.292 | S-adenosyl-L-methionine + 2-chloro-4-nitrophenol | - |
Streptomyces peucetius | S-adenosyl-L-homocysteine + methyl-2-chloro-4-nitrophenol | - |
? | |
2.1.1.292 | S-adenosyl-L-methionine + carminomycin | - |
Streptomyces peucetius | S-adenosyl-L-homocysteine + daunorubicin | - |
? | |
2.1.1.292 | S-adenosyl-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium + 2-chloro-4-nitrophenol | S-adenosyl-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium is a derivative of AdoMet (S-adenosyl-L-methionine) | Streptomyces peucetius | S-adenosyl-(3S)-3-amino-3-(1H-tetrazol-5-yl)propane-1-thiol + methyl-2-chloro-4-nitrophenol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.292 | carminomycin 4-OMT | - |
Streptomyces peucetius |
2.1.1.292 | class I methyltransferase | - |
Streptomyces peucetius |
2.1.1.292 | DnrK | - |
Streptomyces peucetius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 37 | - |
assay at | Streptomyces peucetius |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.292 | 0.00028 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Streptomyces peucetius | |
2.1.1.292 | 0.00032 | - |
2-chloro-4-nitrophenol | pH 8.0, 37°C | Streptomyces peucetius | |
2.1.1.292 | 0.00052 | - |
S-adenosyl-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium | pH 8.0, 37°C | Streptomyces peucetius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.292 | 8 | - |
assay at | Streptomyces peucetius |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.292 | additional information | design, synthesis, and evaluation of stable, functional AdoMet isosteres that are resistant to the primary contributors to AdoMet degradation (depurination, intramolecular cyclization, and sulfonium epimerization). The AdoMet surrogates to serve as competent enzyme cosubstrates and to bind a prototypical class I model methyltransferase (DnrK) in a manner nearly identical to AdoMet. Half-lives of AdoMet derivatives, overview. Analysis of DnrK ligand-bound structures: DnrK-S-adenosyl-(3S)-3-amino-3-(1H-tetrazol-5-yl)propane-1-thiol and DnrK-S-adenosyl-(3S)-3-amino-3-(1H-tetrazol-5-yl)propane-1-thiol-carminomycin | Streptomyces peucetius | |
2.1.1.292 | S-adenosyl-L-methionine | S-adenosyl-L-methionine (AdoMet) is an essential enzyme cosubstrate | Streptomyces peucetius |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.292 | physiological function | the prototypical class I MT DnrK is a model and is the carminomycin 4-O-ethyltransferase that catalyzes a culminating step in the biosynthesis of the anticancer agent daunorubicin in Streptomyces peucetius | Streptomyces peucetius |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.292 | 0.0015 | - |
S-adenosyl-[(3S)-3-amino-3-(1H-tetrazol-5-yl)propyl](methyl)sulfanium | pH 8.0, 37°C | Streptomyces peucetius | |
2.1.1.292 | 0.0021 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Streptomyces peucetius | |
2.1.1.292 | 0.003 | - |
2-chloro-4-nitrophenol | pH 8.0, 37°C | Streptomyces peucetius |