EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.19 | expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. N-227 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.19 | D355R | the mutant shows 164% of wild type activity, and the mutation does not have significant negative effect on stability when compared to the wild type | Bacillus sp. N-227 |
2.4.1.19 | R254F | the mutant shows 196% of wild type activity, and the mutation does not have significant negative effect on stability when compared to the wild type | Bacillus sp. N-227 |
2.4.1.19 | Y127F | the mutant shows 210% of wild type activity, and the mutation does not have significant negative effect on stability when compared to the wild type | Bacillus sp. N-227 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.19 | 15.3 | - |
soluble corn starch | mutant enzyme Y127F, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 17.4 | - |
soluble corn starch | mutant enzyme D355R, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 17.8 | - |
soluble corn starch | mutant enzyme R254F, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 19.2 | - |
soluble corn starch | wild type enzyme, at pH 6.0 and 60°C | Bacillus sp. N-227 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.19 | Bacillus sp. N-227 | Q197W1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.19 | His-tag affinity column chromatography | Bacillus sp. N-227 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.19 | soluble corn starch + glycosyl acceptor | - |
Bacillus sp. N-227 | beta-cyclodextrin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.19 | ? | x * 70000, SDS-PAGE | Bacillus sp. N-227 |
2.4.1.19 | ? | x * 66400, calculated from amino acid sequence | Bacillus sp. N-227 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.19 | beta-CGTase | - |
Bacillus sp. N-227 |
2.4.1.19 | beta-cyclodextrin glucosyltransferase | - |
Bacillus sp. N-227 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.19 | 60 | - |
- |
Bacillus sp. N-227 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.19 | 30 | 60 | at temperatures from 30 to 60°C, the enzyme retains at least 90% of the original activity for at least 1 h. The residual activity at 70°C is about 40%, and the activity decreases to 25% at 80°C | Bacillus sp. N-227 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.19 | 70 | - |
soluble corn starch | wild type enzyme, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 71.37 | - |
soluble corn starch | mutant enzyme D355R, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 72 | - |
soluble corn starch | mutant enzyme R254F, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 73.4 | - |
soluble corn starch | mutant enzyme Y127F, at pH 6.0 and 60°C | Bacillus sp. N-227 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.19 | 6 | - |
- |
Bacillus sp. N-227 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.19 | 5 | 7 | the enzyme is stable for at least 60 min across a wide pH range of 5.0-7.0. The enzyme becomes unstable at higher pH as indicated by the residual activity dropping from 85% at pH 8.0 to less than 30% at pH 10.0 | Bacillus sp. N-227 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.19 | 3.65 | - |
soluble corn starch | wild type enzyme, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 4.04 | - |
soluble corn starch | mutant enzyme R254F, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 4.1 | - |
soluble corn starch | mutant enzyme D355R, at pH 6.0 and 60°C | Bacillus sp. N-227 | |
2.4.1.19 | 4.8 | - |
soluble corn starch | mutant enzyme Y127F, at pH 6.0 and 60°C | Bacillus sp. N-227 |