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Literature summary extracted from

  • Munkhjargal, T.; Ishizaki, T.; Guswanto, A.; Takemae, H.; Yokoyama, N.; Igarashi, I.
    Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target (2016), Vet. Parasitol., 221, 14-23 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.18 medicine recombinant MetAP1 is able to provide considerable protection to mice against a Babesia microti challenge infection based on a reduction in peak parasitemia levels and earlier clearance of the parasite as compared with control mice Babesia bovis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 amastatin 1 mM, 37.6% of initial activity Babesia bovis
3.4.11.18 bestatin 1 mM, 35.9% of initial activity Babesia bovis
3.4.11.18 E-64 1 mM, 89.1% of initial activity Babesia bovis
3.4.11.18 EDTA 1 mM, 37.8% of initial activity Babesia bovis
3.4.11.18 PMSF 1 mM, 82.8% of initial activity Babesia bovis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 0.0143
-
L-methionine 4-methylcoumaryl-7-amide pH 7.5, temperature not specified in the publication Babesia bovis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.18 cytoplasm cytoplasm of merozoite Babesia bovis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.18 Ca2+ 0.1 mM, 120% of initial activity Babesia bovis
3.4.11.18 Mg2+ 0.1 mM, 146% of initial activity Babesia bovis
3.4.11.18 Mn2+ 0.1 mM, 344% of initial activity Babesia bovis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Babesia bovis S6BKQ9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 L-methionine 4-methylcoumaryl-7-amide + H2O
-
Babesia bovis L-methionine + 7-amino-4-methylcoumarin
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.18 ? x * 40867, calculated from sequence, x * 66800, recombinant GST fusion protein Babesia bovis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.18 MetAP1
-
Babesia bovis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.18 7.5
-
-
Babesia bovis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.18 7.5 8.5
-
Babesia bovis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.18 Babesia bovis
-
-
7

General Information

EC Number General Information Comment Organism
3.4.11.18 physiological function recombinant enzyme is immunogenic, eliciting a high antibody titer in mice. Recombinant MetAP1 stimulates the production of IFN-gamma and IL-12 but not IL-4 in the host Babesia bovis