Literature summary extracted from
Munkhjargal, T.; Ishizaki, T.; Guswanto, A.; Takemae, H.; Yokoyama, N.; Igarashi, I.
Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target (2016), Vet. Parasitol., 221, 14-23 .
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.11.18 |
medicine |
recombinant MetAP1 is able to provide considerable protection to mice against a Babesia microti challenge infection based on a reduction in peak parasitemia levels and earlier clearance of the parasite as compared with control mice |
Babesia bovis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.11.18 |
amastatin |
1 mM, 37.6% of initial activity |
Babesia bovis |
|
3.4.11.18 |
bestatin |
1 mM, 35.9% of initial activity |
Babesia bovis |
|
3.4.11.18 |
E-64 |
1 mM, 89.1% of initial activity |
Babesia bovis |
|
3.4.11.18 |
EDTA |
1 mM, 37.8% of initial activity |
Babesia bovis |
|
3.4.11.18 |
PMSF |
1 mM, 82.8% of initial activity |
Babesia bovis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.4.11.18 |
0.0143 |
- |
L-methionine 4-methylcoumaryl-7-amide |
pH 7.5, temperature not specified in the publication |
Babesia bovis |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.4.11.18 |
cytoplasm |
cytoplasm of merozoite |
Babesia bovis |
5737 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.11.18 |
Ca2+ |
0.1 mM, 120% of initial activity |
Babesia bovis |
|
3.4.11.18 |
Mg2+ |
0.1 mM, 146% of initial activity |
Babesia bovis |
|
3.4.11.18 |
Mn2+ |
0.1 mM, 344% of initial activity |
Babesia bovis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.11.18 |
Babesia bovis |
S6BKQ9 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.11.18 |
L-methionine 4-methylcoumaryl-7-amide + H2O |
- |
Babesia bovis |
L-methionine + 7-amino-4-methylcoumarin |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.11.18 |
? |
x * 40867, calculated from sequence, x * 66800, recombinant GST fusion protein |
Babesia bovis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.11.18 |
MetAP1 |
- |
Babesia bovis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.11.18 |
7.5 |
- |
- |
Babesia bovis |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.4.11.18 |
7.5 |
8.5 |
- |
Babesia bovis |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
3.4.11.18 |
Babesia bovis |
- |
- |
7 |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.4.11.18 |
physiological function |
recombinant enzyme is immunogenic, eliciting a high antibody titer in mice. Recombinant MetAP1 stimulates the production of IFN-gamma and IL-12 but not IL-4 in the host |
Babesia bovis |