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Literature summary extracted from

  • Schaeffer, M.L.; Carson, J.D.; Kallender, H.; Lonsdale, J.T.
    Development of a scintillation proximity assay for the Mycobacterium tuberculosis KasA and KasB enzymes involved in mycolic acid biosynthesis (2004), Tuberculosis, 84, 353-360 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.293 DMSO about 130% activity at 2.5% (v/v) DMSO Mycobacterium tuberculosis
2.3.1.294 DMSO about 115% activity at 2.5% (v/v) DMSO Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.293 thiolactomycin
-
 Mycobacterium tuberculosis
2.3.1.294 thiolactomycin
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.293 0.003
-
 myristoyl-[acyl-carrier protein] at pH 6.8 and 37°C Mycobacterium tuberculosis
2.3.1.293 0.005
-
 biotinylated malonyl-[acyl-carrier protein] at pH 6.8 and 37°C Mycobacterium tuberculosis
2.3.1.294 0.0015
-
 myristoyl-[acyl-carrier protein] at pH 7.0 and 37°C Mycobacterium tuberculosis
2.3.1.294 0.005
-
 biotinylated malonyl-[acyl-carrier protein] at pH 7.0 and 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.293 an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Mycobacterium tuberculosis
-
 an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Mycobacterium tuberculosis
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.293 Mycobacterium tuberculosis
-
-
-
2.3.1.294 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.293
-
 Mycobacterium tuberculosis
2.3.1.294
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.293 an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.293 myristoyl-[acyl-carrier protein] + biotinylated malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 myristoyl-[acyl-carrier protein] + biotinylated malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.293 beta-ketoacyl-acyl carrier protein synthase
-
 Mycobacterium tuberculosis
2.3.1.293 KasA
-
 Mycobacterium tuberculosis
2.3.1.294 beta-ketoacyl-acyl carrier protein synthase
-
 Mycobacterium tuberculosis
2.3.1.294 KasB
-
 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.293 0.0047
-
 at pH 6.8 and 37°C Mycobacterium tuberculosis thiolactomycin
2.3.1.294 0.0156
-
 at pH 7.0 and 37°C Mycobacterium tuberculosis thiolactomycin