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Literature summary extracted from

  • Tommasi, S.; Elliot, D.J.; Da Boit, M.; Gray, S.R.; Lewis, B.C.; Mangoni, A.A.
    Homoarginine and inhibition of human arginase activity kinetic characterization and biological relevance (2018), Sci. Rep., 8, 3697 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.1 expression in HEK293T cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.1 agmatine poor arginase 1 inhibitor Homo sapiens
3.5.3.1 asymmetric dimethylarginine poor arginase 1 inhibitor Homo sapiens
3.5.3.1 homoarginine at 2 mM arginine, 18% and 94% inhibition of arginase 2 is observed by homoarginine at the concentration of 1 and 10 mM. At 0.1 mM arginine, the conversion of arginine to ornithine by arginase 2 is inhibited by 47% and 88% in the presence of 1 mM and 10 mM homoarginine. No significant inhibition of arginase 2 is observed at physiological homoarginine concentrations; at Km concentration (3 mM) of the substrate arginine, 1 mM homoarginine inhibits arginase 1 activity by 14%, whereas a concentration of 10 mM results in 50% inhibition of the enzyme. At 0.1 mM arginine, arginase 1 inhibition by 1 mM and 10 mM homoarginine is 30% and 76%, respectively. No significant inhibitory effects of arginase 1 activity are observed with physiological concentrations of homoarginine, 0.001-0.01 mM Homo sapiens
3.5.3.1 lysine at 2 mM arginine, 42% and 67% inhibition of arginase 2 is observed by lasine at 1 mM and 10 mM. At 0.1 mM arginine, the conversion of arginine to ornithine by arginase 2 is inhibited by 44% and 88% in the presence of 1 mM and 10 mM lysine. No significant inhibition of arginase 2 is observed at physiological lysine concentrations; at concentrations of 1 and 10 mM, 39% and 78% inhibition of arginase 1 activity at Km concentration (3 mM) of the substrate arginine, and 44% and 81% inhibition at physiological arginine concentrations. No significant inhibitory effects of arginase 1 activity are observed with physiological concentrations of lysine, 0.1-1.0 mM Homo sapiens
3.5.3.1 Mn2+ the rate of arginase 1-mediated ornithine formation from L-arginine is 20% higher in absence of Mn2+ supplementation; the rate of arginase 1-mediated ornithine formation from L-arginine is 20% higher in absence of Mn2+ supplementation Homo sapiens
3.5.3.1 additional information arginase inhibition is unlikely to play a significant role in the cardio-protective effects of homoarginine; arginase inhibition is unlikely to play a significant role in the cardio-protective effects of homoarginine Homo sapiens
3.5.3.1 NG-monomethyl-L-arginine poor arginase 1 inhibitor Homo sapiens
3.5.3.1 proline poor arginase 1 inhibitor Homo sapiens
3.5.3.1 symmetric dimethylarginine poor arginase 1 inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 1.9
-
 L-arginine arginase 2, pH and temperature not specified in the publication Homo sapiens
3.5.3.1 3.3
-
 L-arginine arginase 1, pH and temperature not specified in the publication Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.1 40000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.1 L-arginine + H2O Homo sapiens
-
 L-ornithine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Homo sapiens P05089
-
-
3.5.3.1 Homo sapiens P78540
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 L-arginine + H2O
-
 Homo sapiens L-ornithine + urea
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.1 ARG1
-
 Homo sapiens
3.5.3.1 ARG2
-
 Homo sapiens
3.5.3.1 arginase-1
-
 Homo sapiens
3.5.3.1 arginase-2
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.1 0.5
-
 lysine arginase 2, pH and temperature not specified in the publication Homo sapiens
3.5.3.1 1.73
-
 homoarginine arginase 2, pH and temperature not specified in the publication Homo sapiens
3.5.3.1 1.79
-
 lysine arginase 1, pH and temperature not specified in the publication Homo sapiens
3.5.3.1 6.1
-
 homoarginine arginase 1, pH and temperature not specified in the publication Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.1 0.88
-
 arginase 2, pH and temperature not specified in the publication Homo sapiens lysine
3.5.3.1 2.52
-
 arginase 2, pH and temperature not specified in the publication Homo sapiens homoarginine
3.5.3.1 3.64
-
 arginase 1, pH and temperature not specified in the publication Homo sapiens lysine
3.5.3.1 8.14
-
 arginase 1, pH and temperature not specified in the publication Homo sapiens homoarginine