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Literature summary extracted from
- Purification, characterization, cytotoxicity and anticancer activities of L-asparaginase, anti-colon cancer protein, from the newly isolated alkaliphilic Streptomyces fradiae NEAE-82 (2016), Sci. Rep., 6, 32926 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | medicine | L-asparaginase may significantly alter the interactions between microvascular endothelial cells, colon cancer cells, and components, resulting in colon cancer cell injury | Streptomyces fradiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 0.01 | - |
L-asparagine | pH 8.6, 37°C | Streptomyces fradiae |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Streptomyces fradiae | - |
- |
- |
| 3.5.1.1 | Streptomyces fradiae NEAE-82 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | - |
Streptomyces fradiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 30.6 | - |
pH 8.6, 37°C | Streptomyces fradiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | - |
Streptomyces fradiae | L-aspartate + NH3 | - |
? | |
| 3.5.1.1 | L-asparagine + H2O | - |
Streptomyces fradiae NEAE-82 | L-aspartate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | ? | x * 53000, SDS-PAGE | Streptomyces fradiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 40 | - |
- |
Streptomyces fradiae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 50 | - |
half-life 185 min | Streptomyces fradiae |
| 3.5.1.1 | 60 | - |
half-life 179 min | Streptomyces fradiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 8.5 | - |
- |
Streptomyces fradiae |
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Release 2023.1 (January 2023)
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