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Literature summary extracted from

  • Rioseras, B.; Yaguee, P.; Lopez-Garcia, M.T.; Gonzalez-Quinonez, N.; Binda, E.; Marinelli, F.; Manteca, A.
    Characterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance, and germination in Streptomyces coelicolor (2016), Sci. Rep., 6, 21659 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.16.4 expression in Escherichia coli Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
3.4.16.4 L684P about 50% of wild-type activity Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.4 penicillin
-
 Streptomyces coelicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.16.4 membrane protein harbors two protein domains separated by a putative hydrophobic transmembrane region Streptomyces coelicolor 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.4 Streptomyces coelicolor Q9KZY7
-
-
3.4.16.4 Streptomyces coelicolor ATCC BAA-471 Q9KZY7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.4 D-Ala-D-Ala + H2O
-
 Streptomyces coelicolor 2 D-Ala
-
 ?
3.4.16.4 D-Ala-D-Ala + H2O
-
 Streptomyces coelicolor ATCC BAA-471 2 D-Ala
-
 ?
3.4.16.4 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O substrate mimics the terminal portions of the peptidoglycan pentapeptide precursors Streptomyces coelicolor Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
3.4.16.4 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O substrate mimics the terminal portions of the peptidoglycan pentapeptide precursors Streptomyces coelicolor ATCC BAA-471 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.4 DD-CPase
-
 Streptomyces coelicolor
3.4.16.4 SCO4439
-
 Streptomyces coelicolor

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.16.4 1
-
 pH 7.2, 37°C Streptomyces coelicolor penicillin

General Information

EC Number General Information Comment Organism
3.4.16.4 physiological function in a gene disruption mutant, the spores are affected in their resistance to heat and acid and show a dramatic increase in swelling during germination. The mycelium of the mutant is more sensitive to glycopeptide antibiotics vancomycin and teicoplanin. Both the DD-CPase domain and the hydrophobic transmembrane region are essential for complementing the wild type phenotypes in the mutant. In a model for the biological mechanism, DD-CPase releases D-Ala from peptidoglycan precursors, thereby reducing the substrate pool for peptidoglycan crosslinking (transpeptidation) Streptomyces coelicolor