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Literature summary extracted from

  • Matovina, M.; Agic, D.; Abramic, M.; Matic, S.; Karacic, Z.; Tomic, S.
    New findings about human dipeptidyl peptidase III based on mutations found in cancer (2017), RSC Adv., 7, 36326-36334 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
3.4.14.4 G313A mutation detected in human cancer, strong decrease in activity. Mutation significantly increases the enzyme flexibility, particularly that of the binding site including the H450ELLGH455 motif, and influences the substrate interactions with the catalytic His568 Homo sapiens
3.4.14.4 G313W mutation detected in human cancer, almost abolishes activity Homo sapiens
3.4.14.4 R510K mutation detected in human cancer, substitution mildly decreases enzyme activity for Arg-Arg-2-naphtylamide substrate Homo sapiens
3.4.14.4 R510Q mutation detected in human cancer, strong decrease in activity Homo sapiens
3.4.14.4 R510W mutation detected in human cancer, almost abolishes activity. Mutation significantly increases the enzyme flexibility, particularly that of the binding site including the H450ELLGH455 motif, and influences the substrate interactions with the catalytic His568 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.4 0.0021
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510Q Homo sapiens
3.4.14.4 0.0041
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510K Homo sapiens
3.4.14.4 0.0045
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510W Homo sapiens
3.4.14.4 0.0055
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, wild-type Homo sapiens
3.4.14.4 0.1146
-
 Arg-Arg-2-naphthylamide pH 7.4, 37°C, mutant G313A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.4 Homo sapiens Q9NY33
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.4 0.026
-
 substrate Ala-Arg-2-naphthylamide, pH 8.6, 37°C Homo sapiens
3.4.14.4 0.027
-
 substrate Phe-Arg-2-naphthylamide, pH 8.6, 37°C Homo sapiens
3.4.14.4 47.9
-
 substrate Arg-Arg-2-naphthylamide, pH 8.6, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.4 Ala-Arg-2-naphthylamide + H2O poor substrate Homo sapiens Ala-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Arg-Arg-2-naphthylamide + H2O preferred substrate Homo sapiens Arg-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Phe-Arg-2-naphthylamide + H2O poor substrate Homo sapiens Phe-Arg + 2-naphthylamine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.4 0.012
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510W Homo sapiens
3.4.14.4 0.224
-
 Arg-Arg-2-naphthylamide pH 7.4, 37°C, mutant G313A Homo sapiens
3.4.14.4 1.35
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510Q Homo sapiens
3.4.14.4 27.53
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510K Homo sapiens
3.4.14.4 35.4
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, wild-type Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.14.4 2
-
 Arg-Arg-2-naphthylamide pH 7.4, 37°C, mutant G313A Homo sapiens
3.4.14.4 3
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510W Homo sapiens
3.4.14.4 864
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510Q Homo sapiens
3.4.14.4 6475
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, wild-type Homo sapiens
3.4.14.4 7066
-
 Arg-Arg-2-naphthylamide pH 8.6, 37°C, mutant R510K Homo sapiens